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Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2.
PLoS One. 2012; 7(2):e30456.Plos

Abstract

Urinary excretion accounts for two-thirds of total elimination of uric acid and the remainder is excreted in feces. However, the mechanism of extra-renal elimination is poorly understood. In the present study, we aimed to clarify the mechanism and the extent of elimination of uric acid through liver and intestine using oxonate-treated rats and Caco-2 cells as a model of human intestinal epithelium. In oxonate-treated rats, significant amounts of externally administered and endogenous uric acid were recovered in the intestinal lumen, while biliary excretion was minimal. Accordingly, direct intestinal secretion was thought to be a substantial contributor to extra-renal elimination of uric acid. Since human efflux transporter BCRP/ABCG2 accepts uric acid as a substrate and genetic polymorphism causing a decrease of BCRP activity is known to be associated with hyperuricemia and gout, the contribution of rBcrp to intestinal secretion was examined. rBcrp was confirmed to transport uric acid in a membrane vesicle study, and intestinal regional differences of expression of rBcrp mRNA were well correlated with uric acid secretory activity into the intestinal lumen. Bcrp1 knockout mice exhibited significantly decreased intestinal secretion and an increased plasma concentration of uric acid. Furthermore, a Bcrp inhibitor, elacridar, caused a decrease of intestinal secretion of uric acid. In Caco-2 cells, uric acid showed a polarized flux from the basolateral to apical side, and this flux was almost abolished in the presence of elacridar. These results demonstrate that BCRP contributes at least in part to the intestinal excretion of uric acid as extra-renal elimination pathway in humans and rats.

Authors+Show Affiliations

Department of Membrane Transport and Biopharmaceutics, Faculty of Pharmacy, Institute of Medical, Pharmaceutical, and Health Sciences, Kanazawa University, Kanazawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22348008

Citation

Hosomi, Atsushi, et al. "Extra-renal Elimination of Uric Acid Via Intestinal Efflux Transporter BCRP/ABCG2." PloS One, vol. 7, no. 2, 2012, pp. e30456.
Hosomi A, Nakanishi T, Fujita T, et al. Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2. PLoS One. 2012;7(2):e30456.
Hosomi, A., Nakanishi, T., Fujita, T., & Tamai, I. (2012). Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2. PloS One, 7(2), e30456. https://doi.org/10.1371/journal.pone.0030456
Hosomi A, et al. Extra-renal Elimination of Uric Acid Via Intestinal Efflux Transporter BCRP/ABCG2. PLoS One. 2012;7(2):e30456. PubMed PMID: 22348008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extra-renal elimination of uric acid via intestinal efflux transporter BCRP/ABCG2. AU - Hosomi,Atsushi, AU - Nakanishi,Takeo, AU - Fujita,Takuya, AU - Tamai,Ikumi, Y1 - 2012/02/10/ PY - 2011/09/28/received PY - 2011/12/20/accepted PY - 2012/2/21/entrez PY - 2012/2/22/pubmed PY - 2012/8/1/medline SP - e30456 EP - e30456 JF - PloS one JO - PLoS One VL - 7 IS - 2 N2 - Urinary excretion accounts for two-thirds of total elimination of uric acid and the remainder is excreted in feces. However, the mechanism of extra-renal elimination is poorly understood. In the present study, we aimed to clarify the mechanism and the extent of elimination of uric acid through liver and intestine using oxonate-treated rats and Caco-2 cells as a model of human intestinal epithelium. In oxonate-treated rats, significant amounts of externally administered and endogenous uric acid were recovered in the intestinal lumen, while biliary excretion was minimal. Accordingly, direct intestinal secretion was thought to be a substantial contributor to extra-renal elimination of uric acid. Since human efflux transporter BCRP/ABCG2 accepts uric acid as a substrate and genetic polymorphism causing a decrease of BCRP activity is known to be associated with hyperuricemia and gout, the contribution of rBcrp to intestinal secretion was examined. rBcrp was confirmed to transport uric acid in a membrane vesicle study, and intestinal regional differences of expression of rBcrp mRNA were well correlated with uric acid secretory activity into the intestinal lumen. Bcrp1 knockout mice exhibited significantly decreased intestinal secretion and an increased plasma concentration of uric acid. Furthermore, a Bcrp inhibitor, elacridar, caused a decrease of intestinal secretion of uric acid. In Caco-2 cells, uric acid showed a polarized flux from the basolateral to apical side, and this flux was almost abolished in the presence of elacridar. These results demonstrate that BCRP contributes at least in part to the intestinal excretion of uric acid as extra-renal elimination pathway in humans and rats. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22348008/Extra_renal_elimination_of_uric_acid_via_intestinal_efflux_transporter_BCRP/ABCG2_ L2 - https://dx.plos.org/10.1371/journal.pone.0030456 DB - PRIME DP - Unbound Medicine ER -