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Identification and validation of a major quantitative trait locus for slow-rusting resistance to stripe rust in wheat.
J Integr Plant Biol. 2012 May; 54(5):330-44.JI

Abstract

Stripe (yellow) rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks (Pst), is one of the most important wheat (Triticum aestivum L.) diseases and causes significant yield losses. A recombinant inbred (RI) population derived from a cross between Yanzhan 1 and Xichang 76-9 cultivars was evaluated for resistance to wheat stripe rust strain CYR32 at both the seedling and adult plant stages. Four resistance quantitative trait loci (QTLs) were detected in this population, in which the major one, designated as Yrq1, was mapped on chromosome 2DS. The strategy of using the Brachypodium distachyon genome, wheat expressed sequence tags and a draft DNA sequences (scaffolds) of the D-genome (Aegilops tauschii Coss.) for the development of simple sequence repeat (SSR) markers was successfully used to identify 147 SSRs in hexaploid wheat. Of the 19 polymorphic SSRs in the RI population, 17 SSRs were mapped in the homeologous group 2 chromosomes near Yrq1 region and eight SSRs were genetically mapped in the 2.7 cM region of Yrq1, providing abundant DNA markers for fine-mapping of Yrq1 and marker-assisted selection in wheat breeding program. The effectiveness of Yrq1 was validated in an independent population, indicating that this resistance QTL can be successfully transferred into a susceptible cultivar for improvement of stripe rust resistance.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Cao X
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Zhou J
No affiliation info available
Gong X
No affiliation info available
Zhao G
No affiliation info available
Jia J
No affiliation info available
Qi X
No affiliation info available

MeSH

BasidiomycotaBrachypodiumChromosome MappingCrosses, GeneticDisease ResistanceGenetic LinkageGenetic MarkersGenome, PlantInbreedingMicrosatellite RepeatsOryzaPhenotypePlant DiseasesPolymorphism, GeneticQuantitative Trait LociRecombination, GeneticReproducibility of ResultsTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22349012

Citation

Cao, Xiaohua, et al. "Identification and Validation of a Major Quantitative Trait Locus for Slow-rusting Resistance to Stripe Rust in Wheat." Journal of Integrative Plant Biology, vol. 54, no. 5, 2012, pp. 330-44.
Cao X, Zhou J, Gong X, et al. Identification and validation of a major quantitative trait locus for slow-rusting resistance to stripe rust in wheat. J Integr Plant Biol. 2012;54(5):330-44.
Cao, X., Zhou, J., Gong, X., Zhao, G., Jia, J., & Qi, X. (2012). Identification and validation of a major quantitative trait locus for slow-rusting resistance to stripe rust in wheat. Journal of Integrative Plant Biology, 54(5), 330-44. https://doi.org/10.1111/j.1744-7909.2012.01111.x
Cao X, et al. Identification and Validation of a Major Quantitative Trait Locus for Slow-rusting Resistance to Stripe Rust in Wheat. J Integr Plant Biol. 2012;54(5):330-44. PubMed PMID: 22349012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and validation of a major quantitative trait locus for slow-rusting resistance to stripe rust in wheat. AU - Cao,Xiaohua, AU - Zhou,Jianghong, AU - Gong,Xiaoping, AU - Zhao,Guangyao, AU - Jia,Jizeng, AU - Qi,Xiaoquan, PY - 2012/2/22/entrez PY - 2012/2/22/pubmed PY - 2012/11/8/medline SP - 330 EP - 44 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 54 IS - 5 N2 - Stripe (yellow) rust, caused by Puccinia striiformis Westend. f. sp. tritici Eriks (Pst), is one of the most important wheat (Triticum aestivum L.) diseases and causes significant yield losses. A recombinant inbred (RI) population derived from a cross between Yanzhan 1 and Xichang 76-9 cultivars was evaluated for resistance to wheat stripe rust strain CYR32 at both the seedling and adult plant stages. Four resistance quantitative trait loci (QTLs) were detected in this population, in which the major one, designated as Yrq1, was mapped on chromosome 2DS. The strategy of using the Brachypodium distachyon genome, wheat expressed sequence tags and a draft DNA sequences (scaffolds) of the D-genome (Aegilops tauschii Coss.) for the development of simple sequence repeat (SSR) markers was successfully used to identify 147 SSRs in hexaploid wheat. Of the 19 polymorphic SSRs in the RI population, 17 SSRs were mapped in the homeologous group 2 chromosomes near Yrq1 region and eight SSRs were genetically mapped in the 2.7 cM region of Yrq1, providing abundant DNA markers for fine-mapping of Yrq1 and marker-assisted selection in wheat breeding program. The effectiveness of Yrq1 was validated in an independent population, indicating that this resistance QTL can be successfully transferred into a susceptible cultivar for improvement of stripe rust resistance. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/22349012/Identification_and_validation_of_a_major_quantitative_trait_locus_for_slow_rusting_resistance_to_stripe_rust_in_wheat_ L2 - https://doi.org/10.1111/j.1744-7909.2012.01111.x DB - PRIME DP - Unbound Medicine ER -