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[Proapoptotic effect of angiotensin II on renal tubular epithelial cells and protective effect of Cordyceps sinensis].
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012 Jan; 37(1):67-72.ZN

Abstract

OBJECTIVE

To investigate the mechanism of the protective effect of Cordyceps sinensis (C. sinensis) on the apoptosis of cultured NRK-52E induced by angiotension II (AngII).

METHODS

NRK-52E cells were incubated with C. sinensis (0, 5, 10, 20, and 40 mg/L) and 10(-8) mol/ L AngII for 24, 48, 72 h. The optimal concentration of C. sinensis was selected. Either NRK-52E cells were incubated with different doses of AngII (0, 10(-12), 10(-10), 10(-8), and 10(-6) mol/L) for 24 h, or with 10(-8) mol/L AngII for 24, 48, and 72 h, to observe the effect of AngII on the apoptosis of NRK- 52E cells. The optimal concentration and time of AngII were selected. In another experiment cells were divided into 5 groups: a control, AngII (10(-8) mol/L), AngII (10(-8) mol/L)+ C. sinensis (40 mg/ L), Ang II (10(-8) mol/L)+ fosinopril (10(-5) mmol/L), and Ang II (10(-8) mol/L)+ fosinopril (10(-5) mol/ L)+C. sinensis (40 mg/L). MTT assay was used to test the changes in the proliferation of NRK-52E cultured with different concentration of C. sinensis for 24, 48, 72 h. The Annecxin V-FITC and PI stainings were applied to detect the apoptosis rate induced by AngII by flow cytometer (FCM) and to determine the eddects of C. sinensis. The activity of caspase-3 was assayed by spectrophotometry.

RESULTS

Certain concentrations of C. sinensis (10-40 mg/L) promoted the proliferation of NRK- 52E cells inhibited by AngII(P<0.05). AngII induced the apoptosis of NRK-52E in a dose and timedependent manner, accompanied with increased activity of caspase-3 (P<0.05). C. sinensis partially suppressed the apoptosis of NRK-52E induced by AngII, and declined the activity of caspase-3 (P<0.05). No significant difference was shown as between the fosinopril group and the fosinopril+C. sinensis group (P>0.05).

CONCLUSION

C. sinensis can suppress the apoptosis of NRK-52E by AngII, and the protective effect of C. sinensis may be inhibiting the activation of caspase-3 during the AngII-induced apoptosis of NRK-52E.

Authors+Show Affiliations

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

22349383

Citation

Tu, Shan, et al. "[Proapoptotic Effect of Angiotensin II On Renal Tubular Epithelial Cells and Protective Effect of Cordyceps Sinensis]." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 37, no. 1, 2012, pp. 67-72.
Tu S, Zhou Q, Tang R, et al. [Proapoptotic effect of angiotensin II on renal tubular epithelial cells and protective effect of Cordyceps sinensis]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012;37(1):67-72.
Tu, S., Zhou, Q., Tang, R., Tang, T., Hu, S., & Ao, X. (2012). [Proapoptotic effect of angiotensin II on renal tubular epithelial cells and protective effect of Cordyceps sinensis]. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 37(1), 67-72. https://doi.org/10.3969/j.issn.1672-7347.2012.01.012
Tu S, et al. [Proapoptotic Effect of Angiotensin II On Renal Tubular Epithelial Cells and Protective Effect of Cordyceps Sinensis]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2012;37(1):67-72. PubMed PMID: 22349383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Proapoptotic effect of angiotensin II on renal tubular epithelial cells and protective effect of Cordyceps sinensis]. AU - Tu,Shan, AU - Zhou,Qiaoling, AU - Tang,Rong, AU - Tang,Tianfeng, AU - Hu,Sai, AU - Ao,Xiang, PY - 2012/2/22/entrez PY - 2012/2/22/pubmed PY - 2013/6/7/medline SP - 67 EP - 72 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 37 IS - 1 N2 - OBJECTIVE: To investigate the mechanism of the protective effect of Cordyceps sinensis (C. sinensis) on the apoptosis of cultured NRK-52E induced by angiotension II (AngII). METHODS: NRK-52E cells were incubated with C. sinensis (0, 5, 10, 20, and 40 mg/L) and 10(-8) mol/ L AngII for 24, 48, 72 h. The optimal concentration of C. sinensis was selected. Either NRK-52E cells were incubated with different doses of AngII (0, 10(-12), 10(-10), 10(-8), and 10(-6) mol/L) for 24 h, or with 10(-8) mol/L AngII for 24, 48, and 72 h, to observe the effect of AngII on the apoptosis of NRK- 52E cells. The optimal concentration and time of AngII were selected. In another experiment cells were divided into 5 groups: a control, AngII (10(-8) mol/L), AngII (10(-8) mol/L)+ C. sinensis (40 mg/ L), Ang II (10(-8) mol/L)+ fosinopril (10(-5) mmol/L), and Ang II (10(-8) mol/L)+ fosinopril (10(-5) mol/ L)+C. sinensis (40 mg/L). MTT assay was used to test the changes in the proliferation of NRK-52E cultured with different concentration of C. sinensis for 24, 48, 72 h. The Annecxin V-FITC and PI stainings were applied to detect the apoptosis rate induced by AngII by flow cytometer (FCM) and to determine the eddects of C. sinensis. The activity of caspase-3 was assayed by spectrophotometry. RESULTS: Certain concentrations of C. sinensis (10-40 mg/L) promoted the proliferation of NRK- 52E cells inhibited by AngII(P<0.05). AngII induced the apoptosis of NRK-52E in a dose and timedependent manner, accompanied with increased activity of caspase-3 (P<0.05). C. sinensis partially suppressed the apoptosis of NRK-52E induced by AngII, and declined the activity of caspase-3 (P<0.05). No significant difference was shown as between the fosinopril group and the fosinopril+C. sinensis group (P>0.05). CONCLUSION: C. sinensis can suppress the apoptosis of NRK-52E by AngII, and the protective effect of C. sinensis may be inhibiting the activation of caspase-3 during the AngII-induced apoptosis of NRK-52E. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/22349383/[Proapoptotic_effect_of_angiotensin_II_on_renal_tubular_epithelial_cells_and_protective_effect_of_Cordyceps_sinensis]_ L2 - http://xbyxb.csu.edu.cn/xbwk/fileup/PDF/20120167.pdf DB - PRIME DP - Unbound Medicine ER -