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A process for high-efficiency isoflavone deglycosylation using Bacillus subtilis natto NTU-18.
Appl Microbiol Biotechnol. 2012 Jun; 94(5):1181-8.AM

Abstract

In order to produce isoflavone aglycosides effectively, a process of isoflavone hydrolysis by Bacillus subtilis natto NTU-18 (BCRC 80390) was established. This process integrates the three stages for the production of isoflavone aglycosides in one single fermenter, including the growth of B. subtilis natto, production of β-glucosidase, deglycosylation of fed isoflavone glycosides. After 8 h of batch culture of B. subtilis natto NTU-18 in 2 L of soy medium, a total of 3 L of soy isoflavone glucoside solution containing 3.0 mg/mL of daidzin and 1.0 mg/mL of genistin was fed continuously over 34 h. The percentage deglycosylation of daidzin and genistin was 97.7% and 94.6%, respectively. The concentration of daidzein and genistein in the broth reached 1,066.8 μg/mL (4.2 mM) and 351 μg/mL (1.3 mM), respectively, and no residual daidzin or genistin was detected. The productivity of the bioconversion of daidzein and genistein over the 42 h of culture was 25.6 mg/L/h and 8.5 mg/L/h, respectively. This showed that this is an efficient bioconversion process for selective estrogen receptor modulator production.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kuo LC
Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan, Republic of China.
Wu RY
No affiliation info available
Lee KT
No affiliation info available

MeSH

Bacillus subtilisBiotransformationCulture MediaFermentationIsoflavonesTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22350317

Citation

Kuo, Lun-Cheng, et al. "A Process for High-efficiency Isoflavone Deglycosylation Using Bacillus Subtilis Natto NTU-18." Applied Microbiology and Biotechnology, vol. 94, no. 5, 2012, pp. 1181-8.
Kuo LC, Wu RY, Lee KT. A process for high-efficiency isoflavone deglycosylation using Bacillus subtilis natto NTU-18. Appl Microbiol Biotechnol. 2012;94(5):1181-8.
Kuo, L. C., Wu, R. Y., & Lee, K. T. (2012). A process for high-efficiency isoflavone deglycosylation using Bacillus subtilis natto NTU-18. Applied Microbiology and Biotechnology, 94(5), 1181-8. https://doi.org/10.1007/s00253-012-3884-8
Kuo LC, Wu RY, Lee KT. A Process for High-efficiency Isoflavone Deglycosylation Using Bacillus Subtilis Natto NTU-18. Appl Microbiol Biotechnol. 2012;94(5):1181-8. PubMed PMID: 22350317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A process for high-efficiency isoflavone deglycosylation using Bacillus subtilis natto NTU-18. AU - Kuo,Lun-Cheng, AU - Wu,Ren-Yu, AU - Lee,Kung-Ta, Y1 - 2012/02/21/ PY - 2011/11/22/received PY - 2011/12/31/accepted PY - 2011/12/29/revised PY - 2012/2/22/entrez PY - 2012/2/22/pubmed PY - 2012/9/6/medline SP - 1181 EP - 8 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 94 IS - 5 N2 - In order to produce isoflavone aglycosides effectively, a process of isoflavone hydrolysis by Bacillus subtilis natto NTU-18 (BCRC 80390) was established. This process integrates the three stages for the production of isoflavone aglycosides in one single fermenter, including the growth of B. subtilis natto, production of β-glucosidase, deglycosylation of fed isoflavone glycosides. After 8 h of batch culture of B. subtilis natto NTU-18 in 2 L of soy medium, a total of 3 L of soy isoflavone glucoside solution containing 3.0 mg/mL of daidzin and 1.0 mg/mL of genistin was fed continuously over 34 h. The percentage deglycosylation of daidzin and genistin was 97.7% and 94.6%, respectively. The concentration of daidzein and genistein in the broth reached 1,066.8 μg/mL (4.2 mM) and 351 μg/mL (1.3 mM), respectively, and no residual daidzin or genistin was detected. The productivity of the bioconversion of daidzein and genistein over the 42 h of culture was 25.6 mg/L/h and 8.5 mg/L/h, respectively. This showed that this is an efficient bioconversion process for selective estrogen receptor modulator production. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/22350317/A_process_for_high_efficiency_isoflavone_deglycosylation_using_Bacillus_subtilis_natto_NTU_18_ L2 - https://dx.doi.org/10.1007/s00253-012-3884-8 DB - PRIME DP - Unbound Medicine ER -