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Proteomic identification of a novel toxin protein (Txp40) from Xenorhabdus nematophila and its insecticidal activity against larvae of Plutella xylostella.
J Agric Food Chem. 2012 Apr 25; 60(16):4053-9.JA

Abstract

For the identification of a novel insecticidal protein, a two-dimensional liquid chromatography (PF-2D) system was used in a quantitative proteomic analysis of Xenorhabdus nematophila CBNU strain isolated from entomophagous nematode Steinernema carpocapsae . Protein patterns obtained from minimum and maximum insecticidal activities during cultivation were contrasted, and a novel toxin protein (Txp40) was identified by MALDI-TOF/MS. The DNA sequence of the cloned toxin gene (1089 bp) has an open reading frame encoding 363 amino acids with a predicted molecular mass of 41162 Da. The txp40 identified in this study is most closely related to the known txp40 cloned from X. nematophila EB (ADQ92844) with 94.4% identical sequence residues. Following the expression of the newly identified toxin gene in Escherichia coli , the insecticidal activity of the recombinant toxin protein was determined against Plutella xylostella larvae; a 56.7% mortality rate was observed within 24 h.

Authors+Show Affiliations

Department of Microbiology, Chungbuk National University, Cheongju, Chungbuk, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22352834

Citation

Park, Jae-Min, et al. "Proteomic Identification of a Novel Toxin Protein (Txp40) From Xenorhabdus Nematophila and Its Insecticidal Activity Against Larvae of Plutella Xylostella." Journal of Agricultural and Food Chemistry, vol. 60, no. 16, 2012, pp. 4053-9.
Park JM, Kim M, Min J, et al. Proteomic identification of a novel toxin protein (Txp40) from Xenorhabdus nematophila and its insecticidal activity against larvae of Plutella xylostella. J Agric Food Chem. 2012;60(16):4053-9.
Park, J. M., Kim, M., Min, J., Lee, S. M., Shin, K. S., Oh, S. D., Oh, S. J., & Kim, Y. H. (2012). Proteomic identification of a novel toxin protein (Txp40) from Xenorhabdus nematophila and its insecticidal activity against larvae of Plutella xylostella. Journal of Agricultural and Food Chemistry, 60(16), 4053-9. https://doi.org/10.1021/jf204351f
Park JM, et al. Proteomic Identification of a Novel Toxin Protein (Txp40) From Xenorhabdus Nematophila and Its Insecticidal Activity Against Larvae of Plutella Xylostella. J Agric Food Chem. 2012 Apr 25;60(16):4053-9. PubMed PMID: 22352834.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic identification of a novel toxin protein (Txp40) from Xenorhabdus nematophila and its insecticidal activity against larvae of Plutella xylostella. AU - Park,Jae-Min, AU - Kim,Mina, AU - Min,Jiho, AU - Lee,Si-Myung, AU - Shin,Kong-Sik, AU - Oh,Sung-Dug, AU - Oh,Suk-Jung, AU - Kim,Yang-Hoon, Y1 - 2012/04/11/ PY - 2012/2/23/entrez PY - 2012/2/23/pubmed PY - 2012/8/11/medline SP - 4053 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 16 N2 - For the identification of a novel insecticidal protein, a two-dimensional liquid chromatography (PF-2D) system was used in a quantitative proteomic analysis of Xenorhabdus nematophila CBNU strain isolated from entomophagous nematode Steinernema carpocapsae . Protein patterns obtained from minimum and maximum insecticidal activities during cultivation were contrasted, and a novel toxin protein (Txp40) was identified by MALDI-TOF/MS. The DNA sequence of the cloned toxin gene (1089 bp) has an open reading frame encoding 363 amino acids with a predicted molecular mass of 41162 Da. The txp40 identified in this study is most closely related to the known txp40 cloned from X. nematophila EB (ADQ92844) with 94.4% identical sequence residues. Following the expression of the newly identified toxin gene in Escherichia coli , the insecticidal activity of the recombinant toxin protein was determined against Plutella xylostella larvae; a 56.7% mortality rate was observed within 24 h. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22352834/Proteomic_identification_of_a_novel_toxin_protein__Txp40__from_Xenorhabdus_nematophila_and_its_insecticidal_activity_against_larvae_of_Plutella_xylostella_ L2 - https://doi.org/10.1021/jf204351f DB - PRIME DP - Unbound Medicine ER -