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Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea.
Antimicrob Agents Chemother. 2012 May; 56(5):2705-12.AA

Abstract

A total of 47 extended-spectrum-cephalosporin-resistant Escherichia coli strains isolated from stray dogs in 2006 and 2007 in the Republic of Korea were investigated using molecular methods. Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase phenotypes were identified in 12 and 23 E. coli isolates, respectively. All 12 ESBL-producing isolates carried bla(CTX-M) genes. The most common CTX-M types were CTX-M-14 (n = 5) and CTX-M-24 (n = 3). Isolates producing CTX-M-3, CTX-M-55, CTX-M-27, and CTX-M-65 were also identified. Twenty-one of 23 AmpC β-lactamase-producing isolates were found to carry bla(CMY-2) genes. TEM-1 was associated with CTX-M and CMY-2 β-lactamases in 4 and 15 isolates, respectively. In addition to bla(TEM-1), two isolates carried bla(DHA-1), and one of them cocarried bla(CMY-2). Both CTX-M and CMY-2 genes were located on large (40 to 170 kb) conjugative plasmids that contained the insertion sequence ISEcp1 upstream of the bla genes. Only in the case of CTX-M genes was there an IS903 sequence downstream of the gene. The spread of ESBLs and AmpC β-lactamases occurred via both horizontal gene transfer, accounting for much of the CTX-M gene dissemination, and clonal spread, accounting for CMY-2 gene dissemination. The horizontal dissemination of bla(CTX-M) and bla(CMY-2) genes was mediated by IncF and IncI1-Iγ plasmids, respectively. The clonal spread of bla(CMY-2) was driven mainly by E. coli strains of virulent phylogroup D lineage ST648. To our knowledge, this is the first report of bla(DHA-1) in E. coli strains isolated from companion animals. This study also represents the first report of CMY-2 β-lactamase-producing E. coli isolates from dogs in the Republic of Korea.

Authors+Show Affiliations

Bacterial Disease Division, Animal, Plant, and Fisheries Quarantine and Inspection Agency, Anyang, Gyeonggi-do, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22354297

Citation

Tamang, Migma Dorji, et al. "Molecular Characterization of Extended-spectrum-β-lactamase-producing and Plasmid-mediated AmpC Β-lactamase-producing Escherichia Coli Isolated From Stray Dogs in South Korea." Antimicrobial Agents and Chemotherapy, vol. 56, no. 5, 2012, pp. 2705-12.
Tamang MD, Nam HM, Jang GC, et al. Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea. Antimicrob Agents Chemother. 2012;56(5):2705-12.
Tamang, M. D., Nam, H. M., Jang, G. C., Kim, S. R., Chae, M. H., Jung, S. C., Byun, J. W., Park, Y. H., & Lim, S. K. (2012). Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea. Antimicrobial Agents and Chemotherapy, 56(5), 2705-12. https://doi.org/10.1128/AAC.05598-11
Tamang MD, et al. Molecular Characterization of Extended-spectrum-β-lactamase-producing and Plasmid-mediated AmpC Β-lactamase-producing Escherichia Coli Isolated From Stray Dogs in South Korea. Antimicrob Agents Chemother. 2012;56(5):2705-12. PubMed PMID: 22354297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of extended-spectrum-β-lactamase-producing and plasmid-mediated AmpC β-lactamase-producing Escherichia coli isolated from stray dogs in South Korea. AU - Tamang,Migma Dorji, AU - Nam,Hyang-Mi, AU - Jang,Geum-Chan, AU - Kim,Su-Ran, AU - Chae,Myung Hwa, AU - Jung,Suk-Chan, AU - Byun,Jae-Won, AU - Park,Yong Ho, AU - Lim,Suk-Kyung, Y1 - 2012/02/21/ PY - 2012/2/23/entrez PY - 2012/2/23/pubmed PY - 2012/8/18/medline SP - 2705 EP - 12 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 56 IS - 5 N2 - A total of 47 extended-spectrum-cephalosporin-resistant Escherichia coli strains isolated from stray dogs in 2006 and 2007 in the Republic of Korea were investigated using molecular methods. Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase phenotypes were identified in 12 and 23 E. coli isolates, respectively. All 12 ESBL-producing isolates carried bla(CTX-M) genes. The most common CTX-M types were CTX-M-14 (n = 5) and CTX-M-24 (n = 3). Isolates producing CTX-M-3, CTX-M-55, CTX-M-27, and CTX-M-65 were also identified. Twenty-one of 23 AmpC β-lactamase-producing isolates were found to carry bla(CMY-2) genes. TEM-1 was associated with CTX-M and CMY-2 β-lactamases in 4 and 15 isolates, respectively. In addition to bla(TEM-1), two isolates carried bla(DHA-1), and one of them cocarried bla(CMY-2). Both CTX-M and CMY-2 genes were located on large (40 to 170 kb) conjugative plasmids that contained the insertion sequence ISEcp1 upstream of the bla genes. Only in the case of CTX-M genes was there an IS903 sequence downstream of the gene. The spread of ESBLs and AmpC β-lactamases occurred via both horizontal gene transfer, accounting for much of the CTX-M gene dissemination, and clonal spread, accounting for CMY-2 gene dissemination. The horizontal dissemination of bla(CTX-M) and bla(CMY-2) genes was mediated by IncF and IncI1-Iγ plasmids, respectively. The clonal spread of bla(CMY-2) was driven mainly by E. coli strains of virulent phylogroup D lineage ST648. To our knowledge, this is the first report of bla(DHA-1) in E. coli strains isolated from companion animals. This study also represents the first report of CMY-2 β-lactamase-producing E. coli isolates from dogs in the Republic of Korea. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/22354297/Molecular_characterization_of_extended_spectrum_β_lactamase_producing_and_plasmid_mediated_AmpC_β_lactamase_producing_Escherichia_coli_isolated_from_stray_dogs_in_South_Korea_ L2 - https://journals.asm.org/doi/10.1128/AAC.05598-11?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -