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Characterization of a Par j 1/Par j 2 mutant hybrid with reduced allergenicity for immunotherapy of Parietaria allergy.
Clin Exp Allergy. 2012 Mar; 42(3):471-80.CE

Abstract

BACKGROUND

Parietaria pollen is one of the major cause of pollinosis in the southern Europe. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease restoring a normal immunity against allergens.

METHODS

We designed a recombinant molecule (PjEDloop1) comprised of genetic-engineered variants of the major allergens of the Parietaria pollen (Par j 2/Par j 1). Purity and chemical-physical properties of the derivative were analysed by RP-HPLC chromatography and Photon Correlation Spectroscopy. Immunological activity was evaluated by means of Western blotting, ELISA inhibition and PBMC proliferation assay in 10 Parietaria allergic patients. Basophil activation was studied in six subjects. The immunogenicity of the hybrid was studied looking at the immune responses induced in a mouse model of sensitization.

RESULTS

The PjEDloop1 hybrid was produced as a purified recombinant protein with high stability in solution. Western blot, ELISA inhibition and basophil activation test showed that the PjEDloop1 displays a remarkable reduced IgE binding and anaphylactic activity. CD3 reactivity was conserved in all patients. Mice immunization with the rPjEDloop1 induced antibodies and T cell responses comparable to that obtained by the wild type allergens. Such antibodies shared the specificities to rPar j 1 and rPar j 2 with human IgE antibodies.

CONCLUSION

Our results demonstrated that a mutant hybrid expressing genetically engineered forms of the major P. judaica allergens displayed reduced allergenicity and retained T cell reactivity for the induction of protective antibodies in vaccination approaches for the treatment of Parietaria pollinosis.

Authors+Show Affiliations

Istituto di Biomedicina ed Immunologia Molecolare, Palermo, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22356145

Citation

Bonura, A, et al. "Characterization of a Par J 1/Par J 2 Mutant Hybrid With Reduced Allergenicity for Immunotherapy of Parietaria Allergy." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 42, no. 3, 2012, pp. 471-80.
Bonura A, Passantino R, Costa MA, et al. Characterization of a Par j 1/Par j 2 mutant hybrid with reduced allergenicity for immunotherapy of Parietaria allergy. Clin Exp Allergy. 2012;42(3):471-80.
Bonura, A., Passantino, R., Costa, M. A., Montana, G., Melis, M., Bondì, M. L., Butteroni, C., Barletta, B., Corinti, S., Di Felice, G., & Colombo, P. (2012). Characterization of a Par j 1/Par j 2 mutant hybrid with reduced allergenicity for immunotherapy of Parietaria allergy. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 42(3), 471-80. https://doi.org/10.1111/j.1365-2222.2011.03938.x
Bonura A, et al. Characterization of a Par J 1/Par J 2 Mutant Hybrid With Reduced Allergenicity for Immunotherapy of Parietaria Allergy. Clin Exp Allergy. 2012;42(3):471-80. PubMed PMID: 22356145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a Par j 1/Par j 2 mutant hybrid with reduced allergenicity for immunotherapy of Parietaria allergy. AU - Bonura,A, AU - Passantino,R, AU - Costa,M A, AU - Montana,G, AU - Melis,M, AU - Bondì,M Luisa, AU - Butteroni,C, AU - Barletta,B, AU - Corinti,S, AU - Di Felice,G, AU - Colombo,P, PY - 2012/2/24/entrez PY - 2012/2/24/pubmed PY - 2012/7/12/medline SP - 471 EP - 80 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin Exp Allergy VL - 42 IS - 3 N2 - BACKGROUND: Parietaria pollen is one of the major cause of pollinosis in the southern Europe. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease restoring a normal immunity against allergens. METHODS: We designed a recombinant molecule (PjEDloop1) comprised of genetic-engineered variants of the major allergens of the Parietaria pollen (Par j 2/Par j 1). Purity and chemical-physical properties of the derivative were analysed by RP-HPLC chromatography and Photon Correlation Spectroscopy. Immunological activity was evaluated by means of Western blotting, ELISA inhibition and PBMC proliferation assay in 10 Parietaria allergic patients. Basophil activation was studied in six subjects. The immunogenicity of the hybrid was studied looking at the immune responses induced in a mouse model of sensitization. RESULTS: The PjEDloop1 hybrid was produced as a purified recombinant protein with high stability in solution. Western blot, ELISA inhibition and basophil activation test showed that the PjEDloop1 displays a remarkable reduced IgE binding and anaphylactic activity. CD3 reactivity was conserved in all patients. Mice immunization with the rPjEDloop1 induced antibodies and T cell responses comparable to that obtained by the wild type allergens. Such antibodies shared the specificities to rPar j 1 and rPar j 2 with human IgE antibodies. CONCLUSION: Our results demonstrated that a mutant hybrid expressing genetically engineered forms of the major P. judaica allergens displayed reduced allergenicity and retained T cell reactivity for the induction of protective antibodies in vaccination approaches for the treatment of Parietaria pollinosis. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/22356145/Characterization_of_a_Par_j_1/Par_j_2_mutant_hybrid_with_reduced_allergenicity_for_immunotherapy_of_Parietaria_allergy_ L2 - https://doi.org/10.1111/j.1365-2222.2011.03938.x DB - PRIME DP - Unbound Medicine ER -