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Analysis of plant hormones by microemulsion electrokinetic capillary chromatography coupled with on-line large volume sample stacking.
Analyst. 2012 Apr 07; 137(7):1723-9.A

Abstract

A novel method of microemulsion electrokinetic capillary chromatography (MEEKC) coupled with on-line large volume sample stacking was developed for the analysis of six plant hormones including indole-3-acetic acid, indole-3-butyric acid, indole-3-propionic acid, 1-naphthaleneacetic acid, abscisic acid and salicylic acid. Baseline separation of six plant hormones was achieved within 10 min by using the microemulsion background electrolyte containing a 97.2% (w/w) 10 mM borate buffer at pH 9.2, 1.0% (w/w) ethyl acetate as oil droplets, 0.6% (w/w) sodium dodecyl sulphate as surfactant and 1.2% (w/w) 1-butanol as cosurfactant. In addition, an on-line concentration method based on a large volume sample stacking technique and multiple wavelength detection was adopted for improving the detection sensitivity in order to determine trace level hormones in a real sample. The optimal method provided about 50-100 fold increase in detection sensitivity compared with a single MEEKC method, and the detection limits (S/N = 3) were between 0.005 and 0.02 μg mL(-1). The proposed method was simple, rapid and sensitive and could be applied to the determination of six plant hormones in spiked water samples, tobacco leaves and 1-naphthylacetic acid in leaf fertilizer. The recoveries ranged from 76.0% to 119.1%, and good reproducibilities were obtained with relative standard deviations (RSDs) less than 6.6%.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Chen Z
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, Fujian, China.
Lin Z
No affiliation info available
Zhang L
No affiliation info available
Cai Y
No affiliation info available
Zhang L
No affiliation info available

MeSH

Chemistry Techniques, AnalyticalChromatography, Micellar Electrokinetic CapillaryMolecular StructurePlant Growth Regulators

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22363931

Citation

Chen, Zongbao, et al. "Analysis of Plant Hormones By Microemulsion Electrokinetic Capillary Chromatography Coupled With On-line Large Volume Sample Stacking." The Analyst, vol. 137, no. 7, 2012, pp. 1723-9.
Chen Z, Lin Z, Zhang L, et al. Analysis of plant hormones by microemulsion electrokinetic capillary chromatography coupled with on-line large volume sample stacking. Analyst. 2012;137(7):1723-9.
Chen, Z., Lin, Z., Zhang, L., Cai, Y., & Zhang, L. (2012). Analysis of plant hormones by microemulsion electrokinetic capillary chromatography coupled with on-line large volume sample stacking. The Analyst, 137(7), 1723-9. https://doi.org/10.1039/c2an16313f
Chen Z, et al. Analysis of Plant Hormones By Microemulsion Electrokinetic Capillary Chromatography Coupled With On-line Large Volume Sample Stacking. Analyst. 2012 Apr 7;137(7):1723-9. PubMed PMID: 22363931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of plant hormones by microemulsion electrokinetic capillary chromatography coupled with on-line large volume sample stacking. AU - Chen,Zongbao, AU - Lin,Zian, AU - Zhang,Lin, AU - Cai,Yan, AU - Zhang,Lan, Y1 - 2012/02/23/ PY - 2012/2/25/entrez PY - 2012/3/1/pubmed PY - 2012/7/27/medline SP - 1723 EP - 9 JF - The Analyst JO - Analyst VL - 137 IS - 7 N2 - A novel method of microemulsion electrokinetic capillary chromatography (MEEKC) coupled with on-line large volume sample stacking was developed for the analysis of six plant hormones including indole-3-acetic acid, indole-3-butyric acid, indole-3-propionic acid, 1-naphthaleneacetic acid, abscisic acid and salicylic acid. Baseline separation of six plant hormones was achieved within 10 min by using the microemulsion background electrolyte containing a 97.2% (w/w) 10 mM borate buffer at pH 9.2, 1.0% (w/w) ethyl acetate as oil droplets, 0.6% (w/w) sodium dodecyl sulphate as surfactant and 1.2% (w/w) 1-butanol as cosurfactant. In addition, an on-line concentration method based on a large volume sample stacking technique and multiple wavelength detection was adopted for improving the detection sensitivity in order to determine trace level hormones in a real sample. The optimal method provided about 50-100 fold increase in detection sensitivity compared with a single MEEKC method, and the detection limits (S/N = 3) were between 0.005 and 0.02 μg mL(-1). The proposed method was simple, rapid and sensitive and could be applied to the determination of six plant hormones in spiked water samples, tobacco leaves and 1-naphthylacetic acid in leaf fertilizer. The recoveries ranged from 76.0% to 119.1%, and good reproducibilities were obtained with relative standard deviations (RSDs) less than 6.6%. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22363931/Analysis_of_plant_hormones_by_microemulsion_electrokinetic_capillary_chromatography_coupled_with_on_line_large_volume_sample_stacking_ L2 - https://doi.org/10.1039/c2an16313f DB - PRIME DP - Unbound Medicine ER -