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Effect of sodium nitroprusside and S-nitrosoglutathione on pigment content and antioxidant system of tocopherol-deficient plants of Arabidopsis thaliana.
Ukr Biokhim Zh (1999). 2011 Nov-Dec; 83(6):69-79.UB

Abstract

Sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO) were used as a source of exogenous nitric oxide (NO) to investigate their effects on biochemical parameters and antioxidant enzyme response in leaves of wild type Columbia and tocopherol-deficient vte4 and vte1 mutant lines of Arabidopsis thaliana plants and possible tocopherol involvement in regulation of antioxidant response under NO-induced stress. SNP enhanced the activity of the enzymes, that scavenge hydrogen peroxide in leaves of all studied lines, and increased glutathione reductase and glutathione-S-transferase activity there. In addition, it decreased the intensity of lipid peroxidation in vte1 mutant line leaves. At the same time, GSNO increased the levels of protein carbonyls and inactivated enzymes ascorbate peroxidase, guaiacol peroxidase and dehydroascorbate reductase in almost all investigated plant lines. In contrast to wild type, GSNO increased superoxide dismutase activity and decreased catalase activity and chlorophyll a/b ratio in the leaves of two mutant lines. It can be assumed that tocopherols in some way are responsible for plant protection against NO-induced stress. However the mechanisms of this protection remain unknown.

Authors+Show Affiliations

Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukraine.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22364021

Citation

Semchuk, N M., et al. "Effect of Sodium Nitroprusside and S-nitrosoglutathione On Pigment Content and Antioxidant System of Tocopherol-deficient Plants of Arabidopsis Thaliana." Ukrains'kyi Biokhimichnyi Zhurnal (1999), vol. 83, no. 6, 2011, pp. 69-79.
Semchuk NM, Vasylyk IuV, Kubrak OI, et al. Effect of sodium nitroprusside and S-nitrosoglutathione on pigment content and antioxidant system of tocopherol-deficient plants of Arabidopsis thaliana. Ukr Biokhim Zh (1999). 2011;83(6):69-79.
Semchuk, N. M., Vasylyk, I. u. V., Kubrak, O. I., & Lushchak, V. I. (2011). Effect of sodium nitroprusside and S-nitrosoglutathione on pigment content and antioxidant system of tocopherol-deficient plants of Arabidopsis thaliana. Ukrains'kyi Biokhimichnyi Zhurnal (1999), 83(6), 69-79.
Semchuk NM, et al. Effect of Sodium Nitroprusside and S-nitrosoglutathione On Pigment Content and Antioxidant System of Tocopherol-deficient Plants of Arabidopsis Thaliana. Ukr Biokhim Zh (1999). 2011 Nov-Dec;83(6):69-79. PubMed PMID: 22364021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of sodium nitroprusside and S-nitrosoglutathione on pigment content and antioxidant system of tocopherol-deficient plants of Arabidopsis thaliana. AU - Semchuk,N M, AU - Vasylyk,Iu V, AU - Kubrak,O I, AU - Lushchak,V I, PY - 2012/2/28/entrez PY - 2012/3/1/pubmed PY - 2012/5/11/medline SP - 69 EP - 79 JF - Ukrains'kyi biokhimichnyi zhurnal (1999) JO - Ukr Biokhim Zh (1999) VL - 83 IS - 6 N2 - Sodium nitroprusside (SNP) and S-nitrosoglutathione (GSNO) were used as a source of exogenous nitric oxide (NO) to investigate their effects on biochemical parameters and antioxidant enzyme response in leaves of wild type Columbia and tocopherol-deficient vte4 and vte1 mutant lines of Arabidopsis thaliana plants and possible tocopherol involvement in regulation of antioxidant response under NO-induced stress. SNP enhanced the activity of the enzymes, that scavenge hydrogen peroxide in leaves of all studied lines, and increased glutathione reductase and glutathione-S-transferase activity there. In addition, it decreased the intensity of lipid peroxidation in vte1 mutant line leaves. At the same time, GSNO increased the levels of protein carbonyls and inactivated enzymes ascorbate peroxidase, guaiacol peroxidase and dehydroascorbate reductase in almost all investigated plant lines. In contrast to wild type, GSNO increased superoxide dismutase activity and decreased catalase activity and chlorophyll a/b ratio in the leaves of two mutant lines. It can be assumed that tocopherols in some way are responsible for plant protection against NO-induced stress. However the mechanisms of this protection remain unknown. UR - https://www.unboundmedicine.com/medline/citation/22364021/Effect_of_sodium_nitroprusside_and_S_nitrosoglutathione_on_pigment_content_and_antioxidant_system_of_tocopherol_deficient_plants_of_Arabidopsis_thaliana_ DB - PRIME DP - Unbound Medicine ER -