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Inhibitory effects of Acorus calamus extracts on mast cell-dependent anaphylactic reactions using mast cell and mouse model.
J Ethnopharmacol. 2012 May 07; 141(1):526-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Acorus calamus Linn. (Araceae) is a traditional herbal plant used for centuries to treat various allergic symptoms including asthma and bronchitis.

AIM OF THE STUDY

The present study was focused to provide a pharmacological basis for the traditional use of Acorus calamus in allergic symptoms using the mast cell-dependent anaphylactic reactions in in vitro and in vivo models.

MATERIALS AND METHODS

Cell viabilities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Dinitrophenyl-human serum albumin (DNP-HSA) induced β-hexosaminidase and interleukin (IL)-4 productions in IgE-sensitized rat basophilic leukaemia (RBL-2H3) cells were measured by enzymatic assay and enzyme-linked immunosorbent assay (ELISA). Passive cutaneous anaphylaxis (PCA) reaction mouse model was implemented for in vivo studies.

RESULTS

Hot water (HW), butylene glycol (BG), hexane (HE) and steam distilled (SD) extracts of Acorus calamus showed different cytoxicity levels evaluated in RBL-2H3 cells. Sub-toxic doses of HW extract suppressed the β-hexosaminidase secretion and IL-4 production significantly and dose dependently in DNP-HSA induced IgE-sensitized RBL-2H3 cells compared to other extracts of Acorus calamus. Further, in vivo studies also revealed that the HW extract significantly inhibited the PCA reaction in mouse compared to the normal control group.

CONCLUSION

HW extract of Acorus calamus most effectively inhibited degranulation and IL-4 secretion in DNP-HSA-stimulated RBL-2H3 cells and also reduced the mast cell-mediated PCA reaction in mouse, providing a therapeutic evidence for its traditional use in ameliorating allergic reactions.

Authors+Show Affiliations

Department of Biotechnology, College of Biomedical & Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22366435

Citation

Kim, Dong-Young, et al. "Inhibitory Effects of Acorus Calamus Extracts On Mast Cell-dependent Anaphylactic Reactions Using Mast Cell and Mouse Model." Journal of Ethnopharmacology, vol. 141, no. 1, 2012, pp. 526-9.
Kim DY, Lee SH, Kim WJ, et al. Inhibitory effects of Acorus calamus extracts on mast cell-dependent anaphylactic reactions using mast cell and mouse model. J Ethnopharmacol. 2012;141(1):526-9.
Kim, D. Y., Lee, S. H., Kim, W. J., Jiang, J., Kim, M. K., Shin, Y. K., Kim, D. W., Moon, W. K., Kwon, S. C., Koppula, S., Kang, T. B., & Lee, K. H. (2012). Inhibitory effects of Acorus calamus extracts on mast cell-dependent anaphylactic reactions using mast cell and mouse model. Journal of Ethnopharmacology, 141(1), 526-9. https://doi.org/10.1016/j.jep.2012.01.043
Kim DY, et al. Inhibitory Effects of Acorus Calamus Extracts On Mast Cell-dependent Anaphylactic Reactions Using Mast Cell and Mouse Model. J Ethnopharmacol. 2012 May 7;141(1):526-9. PubMed PMID: 22366435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of Acorus calamus extracts on mast cell-dependent anaphylactic reactions using mast cell and mouse model. AU - Kim,Dong-Young, AU - Lee,Seung-Hwan, AU - Kim,Wan-Jae, AU - Jiang,Jun, AU - Kim,Myong-Ki, AU - Shin,Yong-Kook, AU - Kim,Dong-Woo, AU - Moon,Won-Kook, AU - Kwon,Sang-Chul, AU - Koppula,Sushruta, AU - Kang,Tae-Bong, AU - Lee,Kwang-Ho, Y1 - 2012/02/17/ PY - 2011/09/27/received PY - 2012/01/19/revised PY - 2012/01/24/accepted PY - 2012/2/28/entrez PY - 2012/3/1/pubmed PY - 2012/8/11/medline SP - 526 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Acorus calamus Linn. (Araceae) is a traditional herbal plant used for centuries to treat various allergic symptoms including asthma and bronchitis. AIM OF THE STUDY: The present study was focused to provide a pharmacological basis for the traditional use of Acorus calamus in allergic symptoms using the mast cell-dependent anaphylactic reactions in in vitro and in vivo models. MATERIALS AND METHODS: Cell viabilities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Dinitrophenyl-human serum albumin (DNP-HSA) induced β-hexosaminidase and interleukin (IL)-4 productions in IgE-sensitized rat basophilic leukaemia (RBL-2H3) cells were measured by enzymatic assay and enzyme-linked immunosorbent assay (ELISA). Passive cutaneous anaphylaxis (PCA) reaction mouse model was implemented for in vivo studies. RESULTS: Hot water (HW), butylene glycol (BG), hexane (HE) and steam distilled (SD) extracts of Acorus calamus showed different cytoxicity levels evaluated in RBL-2H3 cells. Sub-toxic doses of HW extract suppressed the β-hexosaminidase secretion and IL-4 production significantly and dose dependently in DNP-HSA induced IgE-sensitized RBL-2H3 cells compared to other extracts of Acorus calamus. Further, in vivo studies also revealed that the HW extract significantly inhibited the PCA reaction in mouse compared to the normal control group. CONCLUSION: HW extract of Acorus calamus most effectively inhibited degranulation and IL-4 secretion in DNP-HSA-stimulated RBL-2H3 cells and also reduced the mast cell-mediated PCA reaction in mouse, providing a therapeutic evidence for its traditional use in ameliorating allergic reactions. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22366435/Inhibitory_effects_of_Acorus_calamus_extracts_on_mast_cell_dependent_anaphylactic_reactions_using_mast_cell_and_mouse_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00056-6 DB - PRIME DP - Unbound Medicine ER -