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Screening of glycoside isomers in P. scrophulariiflora using ionic liquid-based ultrasonic-assisted extraction and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2012 Apr 15; 26(7):740-8.RC

Abstract

A powerful ionic liquid-based ultrasonic-assisted extraction (ILUAE) method combined with ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/ESI-QTOFMS(n)) was employed in the rapid simultaneous screening of iridoid glycosides, phenylethanoid glycosides, and cucurbitacin glycosides from P. scrophulariiflora. The ILUAE procedure was optimized over several ultrasonic parameters, including the ultrasonic power, concentration of the ionic liquid, and solid-liquid ratio. A comparison with conventional heat-reflux extraction and regular UAE demonstrated that the optimized approach yielded a high extraction efficiency (Picroside I, 2.84%; Picroside II, 3.57%; 6-O-E-feruloyl catalpol, 2.20%) within a short extraction time of 30 min. Negative ion mode ESI-QTOFMS(2) analysis of the fragmentation reactions of the [M-H](-) ions was conducted to characterize the diagnostic ions related to the glycosyl moieties, aglycone units, and the type and substituted position of the ester groups. Interestingly, the positional isomers of the iridoid glycosides could be easily discriminated based on the characteristic ions. A total of 15 glycosides, including three groups of iridoid glycoside isomers and two groups of phenylethanoid glycoside isomers, were conveniently identified within 13.5 min. Moreover, 6'-O-vanilloyl catalpol was identified in P. scrophulariiflora for the first time. The method developed here was further validated by measuring the recovery, correlation coefficient (R(2)), and reproducibility (RSD, n = 5) of three iridoid glycosides: 89.60%-109.02%, 0.9991-0.9998, and 0.93%-1.44%, respectively. This study demonstrated the capabilities of ILUAE combined with UPLC/ESI-QTOFMS(n) for the rapid screening of glycosides in P. scrophulariiflora. This method offers an approach to similar studies on other natural plants.

Authors+Show Affiliations

Research Center of Analysis and Measurement, Zhejiang University of Technology, 18 Chaowang Rd, Hangzhou, Zhejiang, 310014, PR China. xiaojicao@zjut.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22368053

Citation

Cao, Xiaoji, et al. "Screening of Glycoside Isomers in P. Scrophulariiflora Using Ionic Liquid-based Ultrasonic-assisted Extraction and Ultra-performance Liquid Chromatography/electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 26, no. 7, 2012, pp. 740-8.
Cao X, Qiao J, Wang L, et al. Screening of glycoside isomers in P. scrophulariiflora using ionic liquid-based ultrasonic-assisted extraction and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom. 2012;26(7):740-8.
Cao, X., Qiao, J., Wang, L., Ye, X., Zheng, L., Jiang, N., & Mo, W. (2012). Screening of glycoside isomers in P. scrophulariiflora using ionic liquid-based ultrasonic-assisted extraction and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 26(7), 740-8. https://doi.org/10.1002/rcm.6158
Cao X, et al. Screening of Glycoside Isomers in P. Scrophulariiflora Using Ionic Liquid-based Ultrasonic-assisted Extraction and Ultra-performance Liquid Chromatography/electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2012 Apr 15;26(7):740-8. PubMed PMID: 22368053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening of glycoside isomers in P. scrophulariiflora using ionic liquid-based ultrasonic-assisted extraction and ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry. AU - Cao,Xiaoji, AU - Qiao,Jingfang, AU - Wang,Liping, AU - Ye,Xuemin, AU - Zheng,Lebin, AU - Jiang,Nan, AU - Mo,Weimin, PY - 2012/2/28/entrez PY - 2012/3/1/pubmed PY - 2012/6/26/medline SP - 740 EP - 8 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 26 IS - 7 N2 - A powerful ionic liquid-based ultrasonic-assisted extraction (ILUAE) method combined with ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC/ESI-QTOFMS(n)) was employed in the rapid simultaneous screening of iridoid glycosides, phenylethanoid glycosides, and cucurbitacin glycosides from P. scrophulariiflora. The ILUAE procedure was optimized over several ultrasonic parameters, including the ultrasonic power, concentration of the ionic liquid, and solid-liquid ratio. A comparison with conventional heat-reflux extraction and regular UAE demonstrated that the optimized approach yielded a high extraction efficiency (Picroside I, 2.84%; Picroside II, 3.57%; 6-O-E-feruloyl catalpol, 2.20%) within a short extraction time of 30 min. Negative ion mode ESI-QTOFMS(2) analysis of the fragmentation reactions of the [M-H](-) ions was conducted to characterize the diagnostic ions related to the glycosyl moieties, aglycone units, and the type and substituted position of the ester groups. Interestingly, the positional isomers of the iridoid glycosides could be easily discriminated based on the characteristic ions. A total of 15 glycosides, including three groups of iridoid glycoside isomers and two groups of phenylethanoid glycoside isomers, were conveniently identified within 13.5 min. Moreover, 6'-O-vanilloyl catalpol was identified in P. scrophulariiflora for the first time. The method developed here was further validated by measuring the recovery, correlation coefficient (R(2)), and reproducibility (RSD, n = 5) of three iridoid glycosides: 89.60%-109.02%, 0.9991-0.9998, and 0.93%-1.44%, respectively. This study demonstrated the capabilities of ILUAE combined with UPLC/ESI-QTOFMS(n) for the rapid screening of glycosides in P. scrophulariiflora. This method offers an approach to similar studies on other natural plants. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/22368053/Screening_of_glycoside_isomers_in_P__scrophulariiflora_using_ionic_liquid_based_ultrasonic_assisted_extraction_and_ultra_performance_liquid_chromatography/electrospray_ionization_quadrupole_time_of_flight_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.6158 DB - PRIME DP - Unbound Medicine ER -