Tags

Type your tag names separated by a space and hit enter

Total alkaloids of Sophorea alopecuroides-induced down-regulation of AcrAB-TolC efflux pump reverses susceptibility to ciprofloxacin in clinical multidrug resistant Escherichia coli isolates.
Phytother Res 2012; 26(11):1637-43PR

Abstract

In this report, total alkaloids extracted from the seeds of Sophorea alopecuroides (TASA) was evaluated against clinical Escherichia coli isolates resistant to four tested antibiotics, ampicillin (AM), amikacin (AN), cefotaxime (CTX) and ciprofloxacin (CIP). The TASA showed an antibacterial activity against the multidrug resistant (MDR) isolates. In combination with TASA, synergistic effects on the tested antibiotics against the MRD isolates were observed. Similarly, the isolates pretreated with a lower dose of TASA yielded increased and stable susceptibilities to CIP by 16-32-fold determined by a microbroth dilution checkerboard method. Moreover, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed a constitutive overexpression of the AcrAB-TolC pump system in the tested MDR isolates. The pretreatment of MDR isolates with TASA resulted in a statistically down-regulated expression of acrA and acrB genes, and an up-regulated expression of acrR gene (p < 0.05). But the expression of tolC gene was not significantly altered (p > 0.05). These results suggested that the TASA-induced reversal resistance to CIP might be partially through a mechanism of inhibition of the AcrAB-TolC pump activity in these isolates, implying that the TASA can be used as a potential natural source to develop efflux pump inhibitors (EPI) against AcrAB-TolC pump mediated MDR in E. coli isolates.

Authors+Show Affiliations

Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22371352

Citation

Zhou, Xuezhang, et al. "Total Alkaloids of Sophorea Alopecuroides-induced Down-regulation of AcrAB-TolC Efflux Pump Reverses Susceptibility to Ciprofloxacin in Clinical Multidrug Resistant Escherichia Coli Isolates." Phytotherapy Research : PTR, vol. 26, no. 11, 2012, pp. 1637-43.
Zhou X, Jia F, Liu X, et al. Total alkaloids of Sophorea alopecuroides-induced down-regulation of AcrAB-TolC efflux pump reverses susceptibility to ciprofloxacin in clinical multidrug resistant Escherichia coli isolates. Phytother Res. 2012;26(11):1637-43.
Zhou, X., Jia, F., Liu, X., & Wang, Y. (2012). Total alkaloids of Sophorea alopecuroides-induced down-regulation of AcrAB-TolC efflux pump reverses susceptibility to ciprofloxacin in clinical multidrug resistant Escherichia coli isolates. Phytotherapy Research : PTR, 26(11), pp. 1637-43. doi:10.1002/ptr.4623.
Zhou X, et al. Total Alkaloids of Sophorea Alopecuroides-induced Down-regulation of AcrAB-TolC Efflux Pump Reverses Susceptibility to Ciprofloxacin in Clinical Multidrug Resistant Escherichia Coli Isolates. Phytother Res. 2012;26(11):1637-43. PubMed PMID: 22371352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Total alkaloids of Sophorea alopecuroides-induced down-regulation of AcrAB-TolC efflux pump reverses susceptibility to ciprofloxacin in clinical multidrug resistant Escherichia coli isolates. AU - Zhou,Xuezhang, AU - Jia,Fang, AU - Liu,Xiaoming, AU - Wang,Yujiong, Y1 - 2012/02/27/ PY - 2011/08/14/received PY - 2012/01/16/revised PY - 2012/01/16/accepted PY - 2012/2/29/entrez PY - 2012/3/1/pubmed PY - 2013/1/17/medline SP - 1637 EP - 43 JF - Phytotherapy research : PTR JO - Phytother Res VL - 26 IS - 11 N2 - In this report, total alkaloids extracted from the seeds of Sophorea alopecuroides (TASA) was evaluated against clinical Escherichia coli isolates resistant to four tested antibiotics, ampicillin (AM), amikacin (AN), cefotaxime (CTX) and ciprofloxacin (CIP). The TASA showed an antibacterial activity against the multidrug resistant (MDR) isolates. In combination with TASA, synergistic effects on the tested antibiotics against the MRD isolates were observed. Similarly, the isolates pretreated with a lower dose of TASA yielded increased and stable susceptibilities to CIP by 16-32-fold determined by a microbroth dilution checkerboard method. Moreover, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed a constitutive overexpression of the AcrAB-TolC pump system in the tested MDR isolates. The pretreatment of MDR isolates with TASA resulted in a statistically down-regulated expression of acrA and acrB genes, and an up-regulated expression of acrR gene (p < 0.05). But the expression of tolC gene was not significantly altered (p > 0.05). These results suggested that the TASA-induced reversal resistance to CIP might be partially through a mechanism of inhibition of the AcrAB-TolC pump activity in these isolates, implying that the TASA can be used as a potential natural source to develop efflux pump inhibitors (EPI) against AcrAB-TolC pump mediated MDR in E. coli isolates. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/22371352/Total_alkaloids_of_Sophorea_alopecuroides_induced_down_regulation_of_AcrAB_TolC_efflux_pump_reverses_susceptibility_to_ciprofloxacin_in_clinical_multidrug_resistant_Escherichia_coli_isolates_ L2 - https://doi.org/10.1002/ptr.4623 DB - PRIME DP - Unbound Medicine ER -