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Electroacupuncture prevents ovariectomy-induced osteoporosis in rats: a randomised controlled trial.
Acupunct Med. 2012 Mar; 30(1):37-43.AM

Abstract

BACKGROUND

Electroacupuncture (EA) treatment has been shown to increase bone mineral density (BMD) in ovariectomised (OVX) rats; however, the underlying mechanisms remain unclear.

OBJECTIVE

To systematically evaluate the effects of EA on OVX rats and the Wnt/β-catenin signalling pathway.

METHODS

Three-month-old female Sprague-Dawley rats were randomly divided into three different groups (n=10 each): sham operated control (sham operated), ovariectomy (OVX) and ovariectomy with EA treatment (OVX+EA). Rats in the OVX+EA group received 12-week EA treatments.

RESULTS

Serum bone-specific alkaline phosphatase level (p<0.01), BMD of the proximal femoral metaphysis and the fifth lumbar (L5) vertebral body (both, p<0.05) and maximum load and energy to failure of L5 vertebral body (both p<0.01) were significantly higher in the OVX+EA group than in the OVX group. Trabecular area, trabecular width and trabecular number were significantly higher in the OVX+EA group by 66.9%, 29.2% and 30.3%, respectively, than in the OVX group (all, p<0.01). Trabecular separation was 31.9% lower in the OVX+EA group than in the OVX group (p<0.01). Quantitative real-time reverse transcription polymerised chain reaction indicated that the expressions of mRNAs for low-density lipoprotein receptor-related protein 5 and β-catenin were significantly increased in the OVX+EA group, as compared with the OVX group (p<0.01 and p<0.05, respectively).

CONCLUSION

This study demonstrates that EA can prevent OVX-induced bone loss and deterioration of bone architecture and strength by stimulating the Wnt/β-catenin signalling pathway. These findings suggest that EA may bet a promising adjunct method for inhibiting OVX-induced osteoporosis in clinical settings.

Authors+Show Affiliations

Rehabilitation Key Laboratory of Sichuan Province, Department of Rehabilitation, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22378584

Citation

Zhou, Jun, et al. "Electroacupuncture Prevents Ovariectomy-induced Osteoporosis in Rats: a Randomised Controlled Trial." Acupuncture in Medicine : Journal of the British Medical Acupuncture Society, vol. 30, no. 1, 2012, pp. 37-43.
Zhou J, Chen S, Guo H, et al. Electroacupuncture prevents ovariectomy-induced osteoporosis in rats: a randomised controlled trial. Acupunct Med. 2012;30(1):37-43.
Zhou, J., Chen, S., Guo, H., Xia, L., Liu, H., Qin, Y., & He, C. (2012). Electroacupuncture prevents ovariectomy-induced osteoporosis in rats: a randomised controlled trial. Acupuncture in Medicine : Journal of the British Medical Acupuncture Society, 30(1), 37-43. https://doi.org/10.1136/acupmed-2011-010114
Zhou J, et al. Electroacupuncture Prevents Ovariectomy-induced Osteoporosis in Rats: a Randomised Controlled Trial. Acupunct Med. 2012;30(1):37-43. PubMed PMID: 22378584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electroacupuncture prevents ovariectomy-induced osteoporosis in rats: a randomised controlled trial. AU - Zhou,Jun, AU - Chen,Shiju, AU - Guo,Hua, AU - Xia,Lu, AU - Liu,Huifang, AU - Qin,Yuxi, AU - He,Chengqi, PY - 2012/3/2/entrez PY - 2012/3/2/pubmed PY - 2012/7/28/medline SP - 37 EP - 43 JF - Acupuncture in medicine : journal of the British Medical Acupuncture Society JO - Acupunct Med VL - 30 IS - 1 N2 - BACKGROUND: Electroacupuncture (EA) treatment has been shown to increase bone mineral density (BMD) in ovariectomised (OVX) rats; however, the underlying mechanisms remain unclear. OBJECTIVE: To systematically evaluate the effects of EA on OVX rats and the Wnt/β-catenin signalling pathway. METHODS: Three-month-old female Sprague-Dawley rats were randomly divided into three different groups (n=10 each): sham operated control (sham operated), ovariectomy (OVX) and ovariectomy with EA treatment (OVX+EA). Rats in the OVX+EA group received 12-week EA treatments. RESULTS: Serum bone-specific alkaline phosphatase level (p<0.01), BMD of the proximal femoral metaphysis and the fifth lumbar (L5) vertebral body (both, p<0.05) and maximum load and energy to failure of L5 vertebral body (both p<0.01) were significantly higher in the OVX+EA group than in the OVX group. Trabecular area, trabecular width and trabecular number were significantly higher in the OVX+EA group by 66.9%, 29.2% and 30.3%, respectively, than in the OVX group (all, p<0.01). Trabecular separation was 31.9% lower in the OVX+EA group than in the OVX group (p<0.01). Quantitative real-time reverse transcription polymerised chain reaction indicated that the expressions of mRNAs for low-density lipoprotein receptor-related protein 5 and β-catenin were significantly increased in the OVX+EA group, as compared with the OVX group (p<0.01 and p<0.05, respectively). CONCLUSION: This study demonstrates that EA can prevent OVX-induced bone loss and deterioration of bone architecture and strength by stimulating the Wnt/β-catenin signalling pathway. These findings suggest that EA may bet a promising adjunct method for inhibiting OVX-induced osteoporosis in clinical settings. SN - 1759-9873 UR - https://www.unboundmedicine.com/medline/citation/22378584/Electroacupuncture_prevents_ovariectomy_induced_osteoporosis_in_rats:_a_randomised_controlled_trial_ L2 - http://journals.sagepub.com/doi/full/10.1136/acupmed-2011-010114?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -