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Nanospecific inhibition of pyoverdine siderophore production in Pseudomonas chlororaphis O6 by CuO nanoparticles.
Chem Res Toxicol. 2012 May 21; 25(5):1066-74.CR

Abstract

CuO nanoparticles (NPs) exhibit dose-dependent toxicity to bacteria, whereas sublethal concentrations of these NPs change bacterial metabolism. Siderophores are model metabolites to study the impact of sublethal levels of metallic NPs on bacteria because they are involved in survival and interaction with other organisms and with metals. We report that a sublethal level of CuO NPs modify the production of the fluorescent siderophore pyoverdine (PVD) in a soil beneficial bacterium, Pseudomonas chlororaphis O6. The production of PVD was inhibited by CuO NPs but not by bulk CuO nor Cu ions at concentrations equivalent to those released from the NPs. The cell responses occurred despite the NPs forming near micrometer-sized aggregates. The CuO NPs reduced levels of periplasmic and secreted PVD and impaired expression from genes encoding proteins involved in PVD maturation in the periplasm and export through cell membranes. EDTA restored the fluorescence of PVD quenched by Cu ions but did not generate fluorescence with cultures of NP-challenged cells, confirming the absence of PVD. Consequently, depending on the bacterium, this nanoparticle-specific phenomenon mediating cellular reprogramming through effects on secondary metabolism could have an impact on critical environmental processes including bacterial pathogenicity.

Authors+Show Affiliations

Department of Biological Engineering, Utah State University, Logan, UT 84322, United States. cdimkpa@usu.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22380795

Citation

Dimkpa, Christian O., et al. "Nanospecific Inhibition of Pyoverdine Siderophore Production in Pseudomonas Chlororaphis O6 By CuO Nanoparticles." Chemical Research in Toxicology, vol. 25, no. 5, 2012, pp. 1066-74.
Dimkpa CO, McLean JE, Britt DW, et al. Nanospecific inhibition of pyoverdine siderophore production in Pseudomonas chlororaphis O6 by CuO nanoparticles. Chem Res Toxicol. 2012;25(5):1066-74.
Dimkpa, C. O., McLean, J. E., Britt, D. W., Johnson, W. P., Arey, B., Lea, A. S., & Anderson, A. J. (2012). Nanospecific inhibition of pyoverdine siderophore production in Pseudomonas chlororaphis O6 by CuO nanoparticles. Chemical Research in Toxicology, 25(5), 1066-74. https://doi.org/10.1021/tx3000285
Dimkpa CO, et al. Nanospecific Inhibition of Pyoverdine Siderophore Production in Pseudomonas Chlororaphis O6 By CuO Nanoparticles. Chem Res Toxicol. 2012 May 21;25(5):1066-74. PubMed PMID: 22380795.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nanospecific inhibition of pyoverdine siderophore production in Pseudomonas chlororaphis O6 by CuO nanoparticles. AU - Dimkpa,Christian O, AU - McLean,Joan E, AU - Britt,David W, AU - Johnson,William P, AU - Arey,Bruce, AU - Lea,A Scott, AU - Anderson,Anne J, Y1 - 2012/03/09/ PY - 2012/3/3/entrez PY - 2012/3/3/pubmed PY - 2012/9/11/medline SP - 1066 EP - 74 JF - Chemical research in toxicology JO - Chem Res Toxicol VL - 25 IS - 5 N2 - CuO nanoparticles (NPs) exhibit dose-dependent toxicity to bacteria, whereas sublethal concentrations of these NPs change bacterial metabolism. Siderophores are model metabolites to study the impact of sublethal levels of metallic NPs on bacteria because they are involved in survival and interaction with other organisms and with metals. We report that a sublethal level of CuO NPs modify the production of the fluorescent siderophore pyoverdine (PVD) in a soil beneficial bacterium, Pseudomonas chlororaphis O6. The production of PVD was inhibited by CuO NPs but not by bulk CuO nor Cu ions at concentrations equivalent to those released from the NPs. The cell responses occurred despite the NPs forming near micrometer-sized aggregates. The CuO NPs reduced levels of periplasmic and secreted PVD and impaired expression from genes encoding proteins involved in PVD maturation in the periplasm and export through cell membranes. EDTA restored the fluorescence of PVD quenched by Cu ions but did not generate fluorescence with cultures of NP-challenged cells, confirming the absence of PVD. Consequently, depending on the bacterium, this nanoparticle-specific phenomenon mediating cellular reprogramming through effects on secondary metabolism could have an impact on critical environmental processes including bacterial pathogenicity. SN - 1520-5010 UR - https://www.unboundmedicine.com/medline/citation/22380795/Nanospecific_inhibition_of_pyoverdine_siderophore_production_in_Pseudomonas_chlororaphis_O6_by_CuO_nanoparticles_ L2 - https://doi.org/10.1021/tx3000285 DB - PRIME DP - Unbound Medicine ER -