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How to develop a standard operating procedure for sorting unfixed cells.
Methods. 2012 Jul; 57(3):392-7.M

Abstract

Written standard operating procedures (SOPs) are an important tool to assure that recurring tasks in a laboratory are performed in a consistent manner. When the procedure covered in the SOP involves a high-risk activity such as sorting unfixed cells using a jet-in-air sorter, safety elements are critical components of the document. The details on sort sample handling, sorter set-up, validation, operation, troubleshooting, and maintenance, personal protective equipment (PPE), and operator training, outlined in the SOP are to be based on careful risk assessment of the procedure. This review provides background information on the hazards associated with sorting of unfixed cells and the process used to arrive at the appropriate combination of facility design, instrument placement, safety equipment, and practices to be followed.

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Authors+Show Affiliations

Schmid I
David Geffen School of Medicine at UCLA, Department of Hematology/Oncology, Los Angeles, USA. schmid@mednet.ucla.edu

MeSH

AerosolsBlood-Borne PathogensCell SeparationContainment of BiohazardsFacility Design and ConstructionHumansLaboratoriesMaintenanceOccupational HealthProtective DevicesRisk AssessmentSafety Management

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Review

Language

eng

PubMed ID

22381383

Citation

Schmid, Ingrid. "How to Develop a Standard Operating Procedure for Sorting Unfixed Cells." Methods (San Diego, Calif.), vol. 57, no. 3, 2012, pp. 392-7.
Schmid I. How to develop a standard operating procedure for sorting unfixed cells. Methods. 2012;57(3):392-7.
Schmid, I. (2012). How to develop a standard operating procedure for sorting unfixed cells. Methods (San Diego, Calif.), 57(3), 392-7. https://doi.org/10.1016/j.ymeth.2012.02.002
Schmid I. How to Develop a Standard Operating Procedure for Sorting Unfixed Cells. Methods. 2012;57(3):392-7. PubMed PMID: 22381383.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - How to develop a standard operating procedure for sorting unfixed cells. A1 - Schmid,Ingrid, Y1 - 2012/02/21/ PY - 2011/09/28/received PY - 2012/01/27/revised PY - 2012/02/08/accepted PY - 2012/3/3/entrez PY - 2012/3/3/pubmed PY - 2013/1/11/medline SP - 392 EP - 7 JF - Methods (San Diego, Calif.) JO - Methods VL - 57 IS - 3 N2 - Written standard operating procedures (SOPs) are an important tool to assure that recurring tasks in a laboratory are performed in a consistent manner. When the procedure covered in the SOP involves a high-risk activity such as sorting unfixed cells using a jet-in-air sorter, safety elements are critical components of the document. The details on sort sample handling, sorter set-up, validation, operation, troubleshooting, and maintenance, personal protective equipment (PPE), and operator training, outlined in the SOP are to be based on careful risk assessment of the procedure. This review provides background information on the hazards associated with sorting of unfixed cells and the process used to arrive at the appropriate combination of facility design, instrument placement, safety equipment, and practices to be followed. SN - 1095-9130 UR - https://www.unboundmedicine.com/medline/citation/22381383/How_to_develop_a_standard_operating_procedure_for_sorting_unfixed_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1046-2023(12)00026-6 DB - PRIME DP - Unbound Medicine ER -