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Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae.
Mol Cells. 2012 Mar; 33(3):285-93.MC

Abstract

Abscisic acid stress ripening (ASR1) protein is a small hydrophilic, low molecular weight, and stress-specific plant protein. The gene coding region of ASR1 protein, which is induced under high salinity in rice (Oryza sativa Ilmi), was cloned into a yeast expression vector pVTU260 and transformed into yeast cells. Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide (H(2)O(2)), high salinity (NaCl), heat shock, menadione, copper sulfate, sulfuric acid, lactic acid, salicylic acid, and also high concentration of ethanol. In particular, the expression of metabolic enzymes (Fba1p, Pgk1p, Eno2p, Tpi1p, and Adh1p), antioxidant enzyme (Ahp1p), molecular chaperone (Ssb1p), and pyrimidine biosynthesis-related enzyme (Ura1p) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells. All of these enzymes contribute to an alleviated redox state to H2O2-induced oxidative stress. In the in vitro assay, the purified ASR1 protein was able to scavenge ROS by converting H(2)O(2) to H(2)O. Taken together, these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells.

Authors+Show Affiliations

Department of Biology, Kyungpook National University, Daegu 702-701, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22382682

Citation

Kim, Il-Sup, et al. "Rice ASR1 Protein With Reactive Oxygen Species Scavenging and Chaperone-like Activities Enhances Acquired Tolerance to Abiotic Stresses in Saccharomyces Cerevisiae." Molecules and Cells, vol. 33, no. 3, 2012, pp. 285-93.
Kim IS, Kim YS, Yoon HS. Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae. Mol Cells. 2012;33(3):285-93.
Kim, I. S., Kim, Y. S., & Yoon, H. S. (2012). Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae. Molecules and Cells, 33(3), 285-93. https://doi.org/10.1007/s10059-012-2253-x
Kim IS, Kim YS, Yoon HS. Rice ASR1 Protein With Reactive Oxygen Species Scavenging and Chaperone-like Activities Enhances Acquired Tolerance to Abiotic Stresses in Saccharomyces Cerevisiae. Mol Cells. 2012;33(3):285-93. PubMed PMID: 22382682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rice ASR1 protein with reactive oxygen species scavenging and chaperone-like activities enhances acquired tolerance to abiotic stresses in Saccharomyces cerevisiae. AU - Kim,Il-Sup, AU - Kim,Young-Saeng, AU - Yoon,Ho-Sung, Y1 - 2012/02/28/ PY - 2011/11/09/received PY - 2011/12/12/accepted PY - 2011/12/09/revised PY - 2012/3/3/entrez PY - 2012/3/3/pubmed PY - 2012/7/14/medline SP - 285 EP - 93 JF - Molecules and cells JO - Mol Cells VL - 33 IS - 3 N2 - Abscisic acid stress ripening (ASR1) protein is a small hydrophilic, low molecular weight, and stress-specific plant protein. The gene coding region of ASR1 protein, which is induced under high salinity in rice (Oryza sativa Ilmi), was cloned into a yeast expression vector pVTU260 and transformed into yeast cells. Heterologous expression of ASR1 protein in transgenic yeast cells improved tolerance to abiotic stresses including hydrogen peroxide (H(2)O(2)), high salinity (NaCl), heat shock, menadione, copper sulfate, sulfuric acid, lactic acid, salicylic acid, and also high concentration of ethanol. In particular, the expression of metabolic enzymes (Fba1p, Pgk1p, Eno2p, Tpi1p, and Adh1p), antioxidant enzyme (Ahp1p), molecular chaperone (Ssb1p), and pyrimidine biosynthesis-related enzyme (Ura1p) was up-regulated in the transgenic yeast cells under oxidative stress when compared with wild-type cells. All of these enzymes contribute to an alleviated redox state to H2O2-induced oxidative stress. In the in vitro assay, the purified ASR1 protein was able to scavenge ROS by converting H(2)O(2) to H(2)O. Taken together, these results suggest that the ASR1 protein could function as an effective ROS scavenger and its expression could enhance acquired tolerance of ROS-induced oxidative stress through induction of various cell rescue proteins in yeast cells. SN - 0219-1032 UR - https://www.unboundmedicine.com/medline/citation/22382682/Rice_ASR1_protein_with_reactive_oxygen_species_scavenging_and_chaperone_like_activities_enhances_acquired_tolerance_to_abiotic_stresses_in_Saccharomyces_cerevisiae_ L2 - http://www.molcells.org/journal/view.html?year=2012&volume=33&number=3&spage=285 DB - PRIME DP - Unbound Medicine ER -