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House dust bioactivities predict skin prick test reactivity for children with high risk of allergy.

Abstract

BACKGROUND

Although evidence suggests that ambient exposures to endotoxin and other immunostimulants during early life influence allergic risk, efforts to understand this host-environment relationship have been hampered by a paucity of relevant assays.

OBJECTIVES

These investigations determined whether parameters of house dust extract (HDE) bioactivity were predictive of allergen skin prick test (SPT) reactivity for infants at high risk of allergy participating in the Cincinnati Childhood Allergy and Air Pollution Study (CCAAPS).

METHODS

We conducted a nested case-control study, selecting 99 CCAAPS children who had positive SPT results to at least 1 aeroallergen at age 3 years and 101 subjects with negative SPT results. HDEs were prepared from dust samples collected from the subjects' homes at age 1 year. Murine splenocytes and bone marrow-derived dendritic cells were incubated with HDEs, and supernatant cytokine concentrations were determined by means of ELISA. Alternatively, bone marrow-derived dendritic cells were preincubated with HDEs, and then LPS-induced IL-6 responses were assessed. HDE endotoxin levels were determined by using the limulus amebocyte lysate assay.

RESULTS

HDEs derived from the homes of children with positive (cases) and negative (control subjects) SPT results had similar bioactivities. However, when cases were considered in isolation, HDEs with higher levels of bioactivity were significantly associated with children who had lower numbers of positive SPT results. Analogous statistical analyses did not identify any association between HDE endotoxin levels and the aeroallergen sensitization profiles of children included in this study.

CONCLUSION

HDE immunostimulatory activities predicted the aeroallergen sensitization status of CCAAPS subjects better than HDE endotoxin levels. These results provide the first published evidence that HDE bioassays have clinical relevance in predicting atopic risk.

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  • Authors+Show Affiliations

    ,

    Department of Medicine, University of Cincinnati, Cincinnati, Ohio, USA.

    , , , , , ,

    Source

    MeSH

    Allergens
    Animals
    Asthma
    Bone Marrow Cells
    Case-Control Studies
    Child, Preschool
    Cytokines
    Dendritic Cells
    Dust
    Eczema
    Endotoxins
    Female
    Humans
    Hypersensitivity
    Infant
    Interferon-gamma
    Male
    Mice
    Mice, Inbred BALB C
    Prevalence
    Skin Tests
    Spleen

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    22385634

    Citation

    Kim, Haejin, et al. "House Dust Bioactivities Predict Skin Prick Test Reactivity for Children With High Risk of Allergy." The Journal of Allergy and Clinical Immunology, vol. 129, no. 6, 2012, pp. 1529-37.e2.
    Kim H, Tse K, Levin L, et al. House dust bioactivities predict skin prick test reactivity for children with high risk of allergy. J Allergy Clin Immunol. 2012;129(6):1529-37.e2.
    Kim, H., Tse, K., Levin, L., Bernstein, D., Reponen, T., LeMasters, G., ... Horner, A. A. (2012). House dust bioactivities predict skin prick test reactivity for children with high risk of allergy. The Journal of Allergy and Clinical Immunology, 129(6), pp. 1529-37.e2. doi:10.1016/j.jaci.2012.01.051.
    Kim H, et al. House Dust Bioactivities Predict Skin Prick Test Reactivity for Children With High Risk of Allergy. J Allergy Clin Immunol. 2012;129(6):1529-37.e2. PubMed PMID: 22385634.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - House dust bioactivities predict skin prick test reactivity for children with high risk of allergy. AU - Kim,Haejin, AU - Tse,Kevin, AU - Levin,Linda, AU - Bernstein,David, AU - Reponen,Tiina, AU - LeMasters,Grace, AU - Lummus,Zana, AU - Horner,Anthony A, Y1 - 2012/03/03/ PY - 2011/08/03/received PY - 2012/01/12/revised PY - 2012/01/13/accepted PY - 2012/3/6/entrez PY - 2012/3/6/pubmed PY - 2013/1/24/medline SP - 1529 EP - 37.e2 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 129 IS - 6 N2 - BACKGROUND: Although evidence suggests that ambient exposures to endotoxin and other immunostimulants during early life influence allergic risk, efforts to understand this host-environment relationship have been hampered by a paucity of relevant assays. OBJECTIVES: These investigations determined whether parameters of house dust extract (HDE) bioactivity were predictive of allergen skin prick test (SPT) reactivity for infants at high risk of allergy participating in the Cincinnati Childhood Allergy and Air Pollution Study (CCAAPS). METHODS: We conducted a nested case-control study, selecting 99 CCAAPS children who had positive SPT results to at least 1 aeroallergen at age 3 years and 101 subjects with negative SPT results. HDEs were prepared from dust samples collected from the subjects' homes at age 1 year. Murine splenocytes and bone marrow-derived dendritic cells were incubated with HDEs, and supernatant cytokine concentrations were determined by means of ELISA. Alternatively, bone marrow-derived dendritic cells were preincubated with HDEs, and then LPS-induced IL-6 responses were assessed. HDE endotoxin levels were determined by using the limulus amebocyte lysate assay. RESULTS: HDEs derived from the homes of children with positive (cases) and negative (control subjects) SPT results had similar bioactivities. However, when cases were considered in isolation, HDEs with higher levels of bioactivity were significantly associated with children who had lower numbers of positive SPT results. Analogous statistical analyses did not identify any association between HDE endotoxin levels and the aeroallergen sensitization profiles of children included in this study. CONCLUSION: HDE immunostimulatory activities predicted the aeroallergen sensitization status of CCAAPS subjects better than HDE endotoxin levels. These results provide the first published evidence that HDE bioassays have clinical relevance in predicting atopic risk. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/22385634/House_dust_bioactivities_predict_skin_prick_test_reactivity_for_children_with_high_risk_of_allergy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(12)00174-1 DB - PRIME DP - Unbound Medicine ER -