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4-(dimethylamino)butyric acid@PtNPs as enhancer for solid-state electrochemiluminescence aptasensor based on target-induced strand displacement.
Biosens Bioelectron. 2012 Apr 15; 34(1):25-9.BB

Abstract

A solid-state electrochemiluminescence (ECL) aptasensor based on target-induced aptamer displacement for highly sensitive detection of thrombin was developed successfully using 4-(dimethylamino)butyric acid (DMBA)@PtNPs labeling as enhancer. Such a special aptasensor included three main parts: ECL substrate, ECL intensity amplification and target-induced aptamer displacement. The ECL substrate was made by modifying the complex of Pt nanoparticles (PtNPs) and tris(2,2-bipyridyl) ruthenium (II) (Ru(bpy)(3)(2+)) (Ru-PtNPs) onto nafion@multi-walled carbon nanotubes (nafion@MWCNTs) modified electrode surface. A complementary thrombin aptamer labeled by DMBA@PtNPs (Aptamer II) acted as the ECL intensity amplification. The thrombin aptamer (TBA) was applied to hybridize with the labeled complementary thrombin aptamer, yielding a duplex complex of TBA-Aptamer II on the electrode surface. The introduction of thrombin triggered the displacement of Aptamer II from the self-assembled duplex into the solution and the association of inert protein thrombin on the electrode surface, decreasing the amount of DMBA@PtNPs and increasing the electron transfer resistance of the aptasensor and thus resulting large decrease in ECL signal. With the synergistic amplification of DMBA and PtNPs to Ru(bpy)(3)(2+) ECL, the aptasensor showed an enlarged ECL intensity change before and after the detection of thrombin. As a result, the change of ECL intensity has a direct relationship with the logarithm of thrombin concentration in the range of 0.001-30 nM. The detection limit of the proposed aptasensor is 0.4 pM. Thus, the approach is expected to open new opportunities for protein diagnostics in clinical as well as bioanalysis in general.

Authors+Show Affiliations

Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22387036

Citation

Gan, Xianxue, et al. "4-(dimethylamino)butyric acid@PtNPs as Enhancer for Solid-state Electrochemiluminescence Aptasensor Based On Target-induced Strand Displacement." Biosensors & Bioelectronics, vol. 34, no. 1, 2012, pp. 25-9.
Gan X, Yuan R, Chai Y, et al. 4-(dimethylamino)butyric acid@PtNPs as enhancer for solid-state electrochemiluminescence aptasensor based on target-induced strand displacement. Biosens Bioelectron. 2012;34(1):25-9.
Gan, X., Yuan, R., Chai, Y., Yuan, Y., Mao, L., Cao, Y., & Liao, Y. (2012). 4-(dimethylamino)butyric acid@PtNPs as enhancer for solid-state electrochemiluminescence aptasensor based on target-induced strand displacement. Biosensors & Bioelectronics, 34(1), 25-9. https://doi.org/10.1016/j.bios.2011.11.017
Gan X, et al. 4-(dimethylamino)butyric acid@PtNPs as Enhancer for Solid-state Electrochemiluminescence Aptasensor Based On Target-induced Strand Displacement. Biosens Bioelectron. 2012 Apr 15;34(1):25-9. PubMed PMID: 22387036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 4-(dimethylamino)butyric acid@PtNPs as enhancer for solid-state electrochemiluminescence aptasensor based on target-induced strand displacement. AU - Gan,Xianxue, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Yuan,Yali, AU - Mao,Li, AU - Cao,Yaling, AU - Liao,Yuhong, Y1 - 2011/12/03/ PY - 2011/10/10/received PY - 2011/11/09/revised PY - 2011/11/09/accepted PY - 2012/3/6/entrez PY - 2012/3/6/pubmed PY - 2012/8/2/medline SP - 25 EP - 9 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 34 IS - 1 N2 - A solid-state electrochemiluminescence (ECL) aptasensor based on target-induced aptamer displacement for highly sensitive detection of thrombin was developed successfully using 4-(dimethylamino)butyric acid (DMBA)@PtNPs labeling as enhancer. Such a special aptasensor included three main parts: ECL substrate, ECL intensity amplification and target-induced aptamer displacement. The ECL substrate was made by modifying the complex of Pt nanoparticles (PtNPs) and tris(2,2-bipyridyl) ruthenium (II) (Ru(bpy)(3)(2+)) (Ru-PtNPs) onto nafion@multi-walled carbon nanotubes (nafion@MWCNTs) modified electrode surface. A complementary thrombin aptamer labeled by DMBA@PtNPs (Aptamer II) acted as the ECL intensity amplification. The thrombin aptamer (TBA) was applied to hybridize with the labeled complementary thrombin aptamer, yielding a duplex complex of TBA-Aptamer II on the electrode surface. The introduction of thrombin triggered the displacement of Aptamer II from the self-assembled duplex into the solution and the association of inert protein thrombin on the electrode surface, decreasing the amount of DMBA@PtNPs and increasing the electron transfer resistance of the aptasensor and thus resulting large decrease in ECL signal. With the synergistic amplification of DMBA and PtNPs to Ru(bpy)(3)(2+) ECL, the aptasensor showed an enlarged ECL intensity change before and after the detection of thrombin. As a result, the change of ECL intensity has a direct relationship with the logarithm of thrombin concentration in the range of 0.001-30 nM. The detection limit of the proposed aptasensor is 0.4 pM. Thus, the approach is expected to open new opportunities for protein diagnostics in clinical as well as bioanalysis in general. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/22387036/4__dimethylamino_butyric_acid@PtNPs_as_enhancer_for_solid_state_electrochemiluminescence_aptasensor_based_on_target_induced_strand_displacement_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00761-5 DB - PRIME DP - Unbound Medicine ER -