Tags

Type your tag names separated by a space and hit enter

RETRACTED ARTICLE

Aging decreases rate of docosahexaenoic acid synthesis-secretion from circulating unesterified α-linolenic acid by rat liver.
Age (Dordr). 2013 Jun; 35(3):597-608.A

Abstract

Docosahexaenoic acid (DHA, 22:6n-3), an n-3 polyunsaturated fatty acid (PUFA) found at high concentrations in brain and retina and critical to their function, can be obtained from fish products or be synthesized from circulating α-linolenic acid (α-LNA, 18:3n-3) mainly in the liver. With aging, liver synthetic enzymes are reported reduced or unchanged in the rat. To test whether liver synthesis-secretion of DHA from α-LNA changes with age, we measured whole-body DHA conversion coefficients and rates in unanesthetized adult male Fischer-344 rats aged 10, 20, or 30 months, fed an eicosapentaenoic acid (EPA, 20:5n-3)- and DHA-containing diet. Unesterified [U- (13) C]α-LNA bound to albumin was infused intravenously for 2 h, while [(13) C]-esterified n-3 PUFAs were measured in arterial plasma, as were unlabeled unesterified and esterified PUFA concentrations. Plasma unesterified n-3 PUFA concentrations declined with age, but esterified n-3 PUFA concentrations did not change significantly. Calculated conversion coefficients were not changed significantly with age, whereas synthesis-secretion rates (product of conversion coefficient and unesterified plasma α-LNA concentration) of esterified DHA and n-3 DPA were reduced. Turnovers of esterified n-3 PUFAs in plasma decreased with age, whereas half-lives increased. The results suggest that hepatic capacity to synthesize DHA and other n-3 PUFAs from circulating α-LNA is maintained with age in the rat, but that reduced plasma α-LNA availability reduces net synthesis-secretion. As unesterified plasma DHA is the form that is incorporated preferentially into brain phospholipid, its reduced synthesis may be deleterious to brain function in aged rats.

Authors+Show Affiliations

Brain Physiology and Metabolism Section, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892, USA. fgao1@partners.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Intramural
Retracted Publication

Language

eng

PubMed ID

22388930

Citation

Gao, Fei, et al. "Aging Decreases Rate of Docosahexaenoic Acid Synthesis-secretion From Circulating Unesterified Α-linolenic Acid By Rat Liver." Age (Dordrecht, Netherlands), vol. 35, no. 3, 2013, pp. 597-608.
Gao F, Taha AY, Ma K, et al. Aging decreases rate of docosahexaenoic acid synthesis-secretion from circulating unesterified α-linolenic acid by rat liver. Age (Dordr). 2013;35(3):597-608.
Gao, F., Taha, A. Y., Ma, K., Chang, L., Kiesewetter, D., & Rapoport, S. I. (2013). Aging decreases rate of docosahexaenoic acid synthesis-secretion from circulating unesterified α-linolenic acid by rat liver. Age (Dordrecht, Netherlands), 35(3), 597-608. https://doi.org/10.1007/s11357-012-9390-1
Gao F, et al. Aging Decreases Rate of Docosahexaenoic Acid Synthesis-secretion From Circulating Unesterified Α-linolenic Acid By Rat Liver. Age (Dordr). 2013;35(3):597-608. PubMed PMID: 22388930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aging decreases rate of docosahexaenoic acid synthesis-secretion from circulating unesterified α-linolenic acid by rat liver. AU - Gao,Fei, AU - Taha,Ameer Y, AU - Ma,Kaizong, AU - Chang,Lisa, AU - Kiesewetter,Dale, AU - Rapoport,Stanley I, Y1 - 2012/03/03/ PY - 2011/10/23/received PY - 2012/02/08/accepted PY - 2012/3/6/entrez PY - 2012/3/6/pubmed PY - 2013/10/18/medline SP - 597 EP - 608 JF - Age (Dordrecht, Netherlands) JO - Age (Dordr) VL - 35 IS - 3 N2 - Docosahexaenoic acid (DHA, 22:6n-3), an n-3 polyunsaturated fatty acid (PUFA) found at high concentrations in brain and retina and critical to their function, can be obtained from fish products or be synthesized from circulating α-linolenic acid (α-LNA, 18:3n-3) mainly in the liver. With aging, liver synthetic enzymes are reported reduced or unchanged in the rat. To test whether liver synthesis-secretion of DHA from α-LNA changes with age, we measured whole-body DHA conversion coefficients and rates in unanesthetized adult male Fischer-344 rats aged 10, 20, or 30 months, fed an eicosapentaenoic acid (EPA, 20:5n-3)- and DHA-containing diet. Unesterified [U- (13) C]α-LNA bound to albumin was infused intravenously for 2 h, while [(13) C]-esterified n-3 PUFAs were measured in arterial plasma, as were unlabeled unesterified and esterified PUFA concentrations. Plasma unesterified n-3 PUFA concentrations declined with age, but esterified n-3 PUFA concentrations did not change significantly. Calculated conversion coefficients were not changed significantly with age, whereas synthesis-secretion rates (product of conversion coefficient and unesterified plasma α-LNA concentration) of esterified DHA and n-3 DPA were reduced. Turnovers of esterified n-3 PUFAs in plasma decreased with age, whereas half-lives increased. The results suggest that hepatic capacity to synthesize DHA and other n-3 PUFAs from circulating α-LNA is maintained with age in the rat, but that reduced plasma α-LNA availability reduces net synthesis-secretion. As unesterified plasma DHA is the form that is incorporated preferentially into brain phospholipid, its reduced synthesis may be deleterious to brain function in aged rats. SN - 1574-4647 UR - https://www.unboundmedicine.com/medline/citation/22388930/Aging_decreases_rate_of_docosahexaenoic_acid_synthesis_secretion_from_circulating_unesterified_α_linolenic_acid_by_rat_liver_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22388930/ DB - PRIME DP - Unbound Medicine ER -