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Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage.

Abstract

BACKGROUND

Zika virus (ZIKV) is a mosquito-borne flavivirus distributed throughout much of Africa and Asia. Infection with the virus may cause acute febrile illness that clinically resembles dengue fever. A recent study indicated the existence of three geographically distinct viral lineages; however this analysis utilized only a single viral gene. Although ZIKV has been known to circulate in both Africa and Asia since at least the 1950s, little is known about the genetic relationships between geographically distinct virus strains. Moreover, the geographic origin of the strains responsible for the epidemic that occurred on Yap Island, Federated States of Micronesia in 2007, and a 2010 pediatric case in Cambodia, has not been determined.

METHODOLOGY/PRINCIPAL FINDINGS

To elucidate the genetic relationships of geographically distinct ZIKV strains and the origin of the strains responsible for the 2007 outbreak on Yap Island and a 2010 Cambodian pediatric case of ZIKV infection, the nucleotide sequences of the open reading frame of five isolates from Cambodia, Malaysia, Nigeria, Uganda, and Senegal collected between 1947 and 2010 were determined. Phylogenetic analyses of these and previously published ZIKV sequences revealed the existence of two main virus lineages (African and Asian) and that the strain responsible for the Yap epidemic and the Cambodian case most likely originated in Southeast Asia. Examination of the nucleotide and amino acid sequence alignments revealed the loss of a potential glycosylation site in some of the virus strains, which may correlate with the passage history of the virus.

CONCLUSIONS/SIGNIFICANCE

The basal position of the ZIKV strain isolated in Malaysia in 1966 suggests that the recent outbreak in Micronesia was initiated by a strain from Southeast Asia. Because ZIKV infection in humans produces an illness clinically similar to dengue fever and many other tropical infectious diseases, it is likely greatly misdiagnosed and underreported.

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  • Authors+Show Affiliations

    ,

    Institute for Human Infections and Immunity, Center for Tropical Diseases, Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA. adhaddow@gmail.com

    , , , , , , ,

    Source

    MeSH

    Africa
    Animals
    Asia
    Cluster Analysis
    Genotype
    Humans
    Micronesia
    Molecular Epidemiology
    Molecular Sequence Data
    Phylogeography
    RNA, Viral
    Sequence Analysis, DNA
    Zika Virus
    Zika Virus Infection

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, Non-P.H.S.

    Language

    eng

    PubMed ID

    22389730

    Citation

    Haddow, Andrew D., et al. "Genetic Characterization of Zika Virus Strains: Geographic Expansion of the Asian Lineage." PLoS Neglected Tropical Diseases, vol. 6, no. 2, 2012, pp. e1477.
    Haddow AD, Schuh AJ, Yasuda CY, et al. Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. PLoS Negl Trop Dis. 2012;6(2):e1477.
    Haddow, A. D., Schuh, A. J., Yasuda, C. Y., Kasper, M. R., Heang, V., Huy, R., ... Weaver, S. C. (2012). Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. PLoS Neglected Tropical Diseases, 6(2), pp. e1477. doi:10.1371/journal.pntd.0001477.
    Haddow AD, et al. Genetic Characterization of Zika Virus Strains: Geographic Expansion of the Asian Lineage. PLoS Negl Trop Dis. 2012;6(2):e1477. PubMed PMID: 22389730.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. AU - Haddow,Andrew D, AU - Schuh,Amy J, AU - Yasuda,Chadwick Y, AU - Kasper,Matthew R, AU - Heang,Vireak, AU - Huy,Rekol, AU - Guzman,Hilda, AU - Tesh,Robert B, AU - Weaver,Scott C, Y1 - 2012/02/28/ PY - 2011/08/15/received PY - 2011/12/03/accepted PY - 2012/3/6/entrez PY - 2012/3/6/pubmed PY - 2012/6/23/medline SP - e1477 EP - e1477 JF - PLoS neglected tropical diseases JO - PLoS Negl Trop Dis VL - 6 IS - 2 N2 - BACKGROUND: Zika virus (ZIKV) is a mosquito-borne flavivirus distributed throughout much of Africa and Asia. Infection with the virus may cause acute febrile illness that clinically resembles dengue fever. A recent study indicated the existence of three geographically distinct viral lineages; however this analysis utilized only a single viral gene. Although ZIKV has been known to circulate in both Africa and Asia since at least the 1950s, little is known about the genetic relationships between geographically distinct virus strains. Moreover, the geographic origin of the strains responsible for the epidemic that occurred on Yap Island, Federated States of Micronesia in 2007, and a 2010 pediatric case in Cambodia, has not been determined. METHODOLOGY/PRINCIPAL FINDINGS: To elucidate the genetic relationships of geographically distinct ZIKV strains and the origin of the strains responsible for the 2007 outbreak on Yap Island and a 2010 Cambodian pediatric case of ZIKV infection, the nucleotide sequences of the open reading frame of five isolates from Cambodia, Malaysia, Nigeria, Uganda, and Senegal collected between 1947 and 2010 were determined. Phylogenetic analyses of these and previously published ZIKV sequences revealed the existence of two main virus lineages (African and Asian) and that the strain responsible for the Yap epidemic and the Cambodian case most likely originated in Southeast Asia. Examination of the nucleotide and amino acid sequence alignments revealed the loss of a potential glycosylation site in some of the virus strains, which may correlate with the passage history of the virus. CONCLUSIONS/SIGNIFICANCE: The basal position of the ZIKV strain isolated in Malaysia in 1966 suggests that the recent outbreak in Micronesia was initiated by a strain from Southeast Asia. Because ZIKV infection in humans produces an illness clinically similar to dengue fever and many other tropical infectious diseases, it is likely greatly misdiagnosed and underreported. SN - 1935-2735 UR - https://www.unboundmedicine.com/medline/citation/22389730/Genetic_characterization_of_Zika_virus_strains:_geographic_expansion_of_the_Asian_lineage_ L2 - http://dx.plos.org/10.1371/journal.pntd.0001477 DB - PRIME DP - Unbound Medicine ER -