Tags

Type your tag names separated by a space and hit enter

Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes.
Mol Cell Biol. 2012 May; 32(9):1710-9.MC

Abstract

Dosage compensation equalizes X-linked gene expression between the sexes. This process is achieved in Caenorhabditis elegans by hermaphrodite-specific, dosage compensation complex (DCC)-mediated, 2-fold X chromosome downregulation. How the DCC downregulates gene expression is not known. By analyzing the distribution of histone modifications in nuclei using quantitative fluorescence microscopy, we found that H4K16 acetylation (H4K16ac) is underrepresented and H4K20 monomethylation (H4K20me1) is enriched on hermaphrodite X chromosomes in a DCC-dependent manner. Depletion of H4K16ac also requires the conserved histone deacetylase SIR-2.1, while enrichment of H4K20me1 requires the activities of the histone methyltransferases SET-1 and SET-4. Our data suggest that the mechanism of dosage compensation in C. elegans involves redistribution of chromatin-modifying activities, leading to a depletion of H4K16ac and an enrichment of H4K20me1 on the X chromosomes. These results support conserved roles for histone H4 chromatin modification in worm dosage compensation analogous to those seen in flies, using similar elements and opposing strategies to achieve differential 2-fold changes in X-linked gene expression.

Authors+Show Affiliations

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22393255

Citation

Wells, Michael B., et al. "Caenorhabditis Elegans Dosage Compensation Regulates Histone H4 Chromatin State On X Chromosomes." Molecular and Cellular Biology, vol. 32, no. 9, 2012, pp. 1710-9.
Wells MB, Snyder MJ, Custer LM, et al. Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes. Mol Cell Biol. 2012;32(9):1710-9.
Wells, M. B., Snyder, M. J., Custer, L. M., & Csankovszki, G. (2012). Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes. Molecular and Cellular Biology, 32(9), 1710-9. https://doi.org/10.1128/MCB.06546-11
Wells MB, et al. Caenorhabditis Elegans Dosage Compensation Regulates Histone H4 Chromatin State On X Chromosomes. Mol Cell Biol. 2012;32(9):1710-9. PubMed PMID: 22393255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes. AU - Wells,Michael B, AU - Snyder,Martha J, AU - Custer,Laura M, AU - Csankovszki,Gyorgyi, Y1 - 2012/03/05/ PY - 2012/3/7/entrez PY - 2012/3/7/pubmed PY - 2012/6/26/medline SP - 1710 EP - 9 JF - Molecular and cellular biology JO - Mol. Cell. Biol. VL - 32 IS - 9 N2 - Dosage compensation equalizes X-linked gene expression between the sexes. This process is achieved in Caenorhabditis elegans by hermaphrodite-specific, dosage compensation complex (DCC)-mediated, 2-fold X chromosome downregulation. How the DCC downregulates gene expression is not known. By analyzing the distribution of histone modifications in nuclei using quantitative fluorescence microscopy, we found that H4K16 acetylation (H4K16ac) is underrepresented and H4K20 monomethylation (H4K20me1) is enriched on hermaphrodite X chromosomes in a DCC-dependent manner. Depletion of H4K16ac also requires the conserved histone deacetylase SIR-2.1, while enrichment of H4K20me1 requires the activities of the histone methyltransferases SET-1 and SET-4. Our data suggest that the mechanism of dosage compensation in C. elegans involves redistribution of chromatin-modifying activities, leading to a depletion of H4K16ac and an enrichment of H4K20me1 on the X chromosomes. These results support conserved roles for histone H4 chromatin modification in worm dosage compensation analogous to those seen in flies, using similar elements and opposing strategies to achieve differential 2-fold changes in X-linked gene expression. SN - 1098-5549 UR - https://www.unboundmedicine.com/medline/citation/22393255/Caenorhabditis_elegans_dosage_compensation_regulates_histone_H4_chromatin_state_on_X_chromosomes_ L2 - http://mcb.asm.org/cgi/pmidlookup?view=long&pmid=22393255 DB - PRIME DP - Unbound Medicine ER -