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Protective effects of garlic oil on hepatocarcinoma induced by N-nitrosodiethylamine in rats.
Int J Biol Sci 2012; 8(3):363-74IJ

Abstract

To investigate the protective effects and the possible mechanisms of garlic oil (GO) against N-nitrosodiethylamine (NDEA)-induced hepatocarcinoma in rats, Wistar rats were gavaged with GO (20 or 40 mg/kg) for 1 week, and then were gavaged with GO and NDEA (10 mg/kg) for the next 20 weeks. The changes of morphology, histology, the biochemical indices of serum, and DNA oxidative damage of liver were examined to assess the protective effects. Lipid peroxidation (LPO), antioxidant defense system, and apoptosis-related proteins were measured to investigate potential mechanisms. At the end of the study (21 weeks), GO administration significantly inhibited the increase of the nodule incidence and average nodule number per nodule-bearing liver induced by NDEA, improved hepatocellular architecture, and dramatically inhibited NDEA-induced elevation of serum biochemical indices (alanine aminotransferase , aspartate aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase) and hepatic 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in a dose-dependent manner. The mechanistic studies demonstrated that GO counteracted NDEA-induced oxidative stress in rats illustrated by the restoration of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) levels, and the reduction of the malondialdehyde (MDA) levels in liver. Furthermore, the mRNA and protein levels of Bcl-2, Bcl-xl, andβ-arrestin-2 were significantly decreased whereas those of Bax and caspase-3 were significantly increased. These data suggest that GO exhibited significant protection against NDEA-induced hepatocarcinogenesis, which might be related with the enhancement of the antioxidant activity and the induction of apoptosis.

Authors+Show Affiliations

Institute of Toxicology, School of Public Health, Shandong University, Jinan, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22393308

Citation

Zhang, Cui-Li, et al. "Protective Effects of Garlic Oil On Hepatocarcinoma Induced By N-nitrosodiethylamine in Rats." International Journal of Biological Sciences, vol. 8, no. 3, 2012, pp. 363-74.
Zhang CL, Zeng T, Zhao XL, et al. Protective effects of garlic oil on hepatocarcinoma induced by N-nitrosodiethylamine in rats. Int J Biol Sci. 2012;8(3):363-74.
Zhang, C. L., Zeng, T., Zhao, X. L., Yu, L. H., Zhu, Z. P., & Xie, K. Q. (2012). Protective effects of garlic oil on hepatocarcinoma induced by N-nitrosodiethylamine in rats. International Journal of Biological Sciences, 8(3), pp. 363-74. doi:10.7150/ijbs.3796.
Zhang CL, et al. Protective Effects of Garlic Oil On Hepatocarcinoma Induced By N-nitrosodiethylamine in Rats. Int J Biol Sci. 2012;8(3):363-74. PubMed PMID: 22393308.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of garlic oil on hepatocarcinoma induced by N-nitrosodiethylamine in rats. AU - Zhang,Cui-Li, AU - Zeng,Tao, AU - Zhao,Xiu-Lan, AU - Yu,Li-Hua, AU - Zhu,Zhen-Ping, AU - Xie,Ke-Qin, Y1 - 2012/02/22/ PY - 2011/11/14/received PY - 2012/02/16/accepted PY - 2012/3/7/entrez PY - 2012/3/7/pubmed PY - 2012/7/18/medline KW - Antioxidant KW - Apoptosis KW - Garlic oil KW - Hepatocarcinoma KW - Nitrosodiethylamine SP - 363 EP - 74 JF - International journal of biological sciences JO - Int. J. Biol. Sci. VL - 8 IS - 3 N2 - To investigate the protective effects and the possible mechanisms of garlic oil (GO) against N-nitrosodiethylamine (NDEA)-induced hepatocarcinoma in rats, Wistar rats were gavaged with GO (20 or 40 mg/kg) for 1 week, and then were gavaged with GO and NDEA (10 mg/kg) for the next 20 weeks. The changes of morphology, histology, the biochemical indices of serum, and DNA oxidative damage of liver were examined to assess the protective effects. Lipid peroxidation (LPO), antioxidant defense system, and apoptosis-related proteins were measured to investigate potential mechanisms. At the end of the study (21 weeks), GO administration significantly inhibited the increase of the nodule incidence and average nodule number per nodule-bearing liver induced by NDEA, improved hepatocellular architecture, and dramatically inhibited NDEA-induced elevation of serum biochemical indices (alanine aminotransferase , aspartate aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase) and hepatic 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in a dose-dependent manner. The mechanistic studies demonstrated that GO counteracted NDEA-induced oxidative stress in rats illustrated by the restoration of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) levels, and the reduction of the malondialdehyde (MDA) levels in liver. Furthermore, the mRNA and protein levels of Bcl-2, Bcl-xl, andβ-arrestin-2 were significantly decreased whereas those of Bax and caspase-3 were significantly increased. These data suggest that GO exhibited significant protection against NDEA-induced hepatocarcinogenesis, which might be related with the enhancement of the antioxidant activity and the induction of apoptosis. SN - 1449-2288 UR - https://www.unboundmedicine.com/medline/citation/22393308/Protective_effects_of_garlic_oil_on_hepatocarcinoma_induced_by_N_nitrosodiethylamine_in_rats_ L2 - http://www.ijbs.com/v08p0363.htm DB - PRIME DP - Unbound Medicine ER -