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A natural antisense transcript regulates mucD gene expression and biofilm biosynthesis in Pseudomonas aeruginosa.


Natural antisense transcripts (NATs) are naturally widespread and have very important functions in regulating expression of their target genes. In this report, we identified a novel NAT, designated mucD-AS, transcribed from the opposite strand ofmucD gene locus, which has a pivotal function in regulating the biosynthesis of alginate. For the biotechnological and medical significance in the study of alginate biosynthesis, we investigated the effect ofmucD-AS on mucD expression and alginate biosynthesis, and confirmed mucD-AS can induce biofilm formation ofP. aeruginosa significantly. Since alginate has a key function in the infection process and acts as virulence factor of P. aeruginosa, we speculate mucD-AS may have a regulatory function in this process.

Authors+Show Affiliations


Department of Microbiology, College of Medicine, The Third Military Medical University, Key Lab of Microbial Engineering Under the Educational Committee in Chongqing, Chong Qing 400038, China.

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Mikrobiologiia 80:6 pg 756-62


Bacterial Proteins
Base Sequence
Blotting, Northern
Gene Expression Regulation, Bacterial
Genes, Bacterial
Glucuronic Acid
Hexuronic Acids
Molecular Sequence Data
Pseudomonas aeruginosa
RNA, Antisense
Sigma Factor
Transcription, Genetic
Virulence Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't



PubMed ID