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Decellularized rabbit cricoarytenoid dorsalis muscle for laryngeal regeneration.
OBJECTIVESAlthough considerable progress has been made in regenerative medicine, a quantum step would be the replacement and/or regeneration of functional muscle tissue. For example, although patients' airways can now be successfully replaced with stem cell-based techniques, a much greater patient need would be addressed by regeneration of the muscles required for engineering a functional larynx, in which active movement is critical. The rabbit cricoarytenoid dorsalis muscle was chosen for the present study because it is equivalent to the posterior cricoarytenoid muscle, the only significant abductor muscle in human larynges.
METHODSRabbit cricoarytenoid dorsalis muscles were harvested, and different decellularization methods were compared by use of a combination of histologic, immunohistochemical, and molecular techniques. Decellularized scaffolds were implanted into Sprague-Dawley rats as part of a 2-week biocompatibility study to assess immunogenicity.
RESULTSDecellularization with a combination of latrunculin B, potassium iodide, potassium chloride, and deoxyribonuclease resulted in total DNA clearance and reduced levels of major histocompatibility complex class II expression, with relative preservation of the scaffold's structural integrity (collagen, elastin, and glycosaminoglycan content). The scaffolds showed minimal signs of rejection at 2 weeks in a cross-species (xenotransplantation) study.
CONCLUSIONSDecellularized laryngeal muscles, which are nonimmunogenic, may provide the optimal scaffold source for the generation of a fully functional tissue-engineered larynx.
University College London Institute of Child Health, 30 Guilford St, London WC1N 1EH, United Kingdom., , ,
The Annals of otology, rhinology, and laryngology 121:2 2012 Feb pg 129-38
Bicyclo Compounds, Heterocyclic
Histocompatibility Antigens Class II
Sodium Dodecyl Sulfate
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't