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Highly sensitive detection of protein and small molecules based on aptamer-modified electrochemiluminescence nanoprobe.
Analyst. 2012 Apr 21; 137(8):1963-9.A

Abstract

Amplified optical detection of biomolecules using nanoparticle as the carrier has attracted considerable interest in the scientific community. In this study, a promising aptasensor was developed for highly sensitive detection of protein and small molecules based on the construction of aptamer-modified electrochemiluminescence (ECL) nanoprobe. Specifically, thrombin and ATP serve as the examples for detection. By taking advantage of sandwich binding of two affinity aptamers for high specificity, tris-(2,2'-bipyridyl)ruthenium (TBR)-cysteamine loaded in gold nanoparticle (GNP) as barcodes for signal amplification, and micromagnetic particles (MMPs) based ECL technology for rapid detection, a novel assay for biomolecules quantification was developed. The sandwich complex containing targets could be selectively captured by MMPs and then quantified by ECL intensity. We have demonstrated that the detection limits of human thrombin and ATP are 1 pM and 10 pM, respectively, with high specificity. The proposed technology is expected to become a powerful tool for biomolecule analysis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhou X
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
Duan R
No affiliation info available
Xing D
No affiliation info available

MeSH

Aptamers, NucleotideElectrochemical TechniquesLimit of DetectionLuminescenceProteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22400127

Citation

Zhou, Xiaoming, et al. "Highly Sensitive Detection of Protein and Small Molecules Based On Aptamer-modified Electrochemiluminescence Nanoprobe." The Analyst, vol. 137, no. 8, 2012, pp. 1963-9.
Zhou X, Duan R, Xing D. Highly sensitive detection of protein and small molecules based on aptamer-modified electrochemiluminescence nanoprobe. Analyst. 2012;137(8):1963-9.
Zhou, X., Duan, R., & Xing, D. (2012). Highly sensitive detection of protein and small molecules based on aptamer-modified electrochemiluminescence nanoprobe. The Analyst, 137(8), 1963-9. https://doi.org/10.1039/c2an00020b
Zhou X, Duan R, Xing D. Highly Sensitive Detection of Protein and Small Molecules Based On Aptamer-modified Electrochemiluminescence Nanoprobe. Analyst. 2012 Apr 21;137(8):1963-9. PubMed PMID: 22400127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Highly sensitive detection of protein and small molecules based on aptamer-modified electrochemiluminescence nanoprobe. AU - Zhou,Xiaoming, AU - Duan,Ruixue, AU - Xing,Da, Y1 - 2012/03/08/ PY - 2012/3/9/entrez PY - 2012/3/9/pubmed PY - 2012/7/10/medline SP - 1963 EP - 9 JF - The Analyst JO - Analyst VL - 137 IS - 8 N2 - Amplified optical detection of biomolecules using nanoparticle as the carrier has attracted considerable interest in the scientific community. In this study, a promising aptasensor was developed for highly sensitive detection of protein and small molecules based on the construction of aptamer-modified electrochemiluminescence (ECL) nanoprobe. Specifically, thrombin and ATP serve as the examples for detection. By taking advantage of sandwich binding of two affinity aptamers for high specificity, tris-(2,2'-bipyridyl)ruthenium (TBR)-cysteamine loaded in gold nanoparticle (GNP) as barcodes for signal amplification, and micromagnetic particles (MMPs) based ECL technology for rapid detection, a novel assay for biomolecules quantification was developed. The sandwich complex containing targets could be selectively captured by MMPs and then quantified by ECL intensity. We have demonstrated that the detection limits of human thrombin and ATP are 1 pM and 10 pM, respectively, with high specificity. The proposed technology is expected to become a powerful tool for biomolecule analysis. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22400127/Highly_sensitive_detection_of_protein_and_small_molecules_based_on_aptamer_modified_electrochemiluminescence_nanoprobe_ L2 - https://doi.org/10.1039/c2an00020b DB - PRIME DP - Unbound Medicine ER -