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Rapid determination of endogenous cytokinins in plant samples by combination of magnetic solid phase extraction with hydrophilic interaction chromatography-tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 01; 891-892:27-35.JC

Abstract

A 2-acrylamido-2-methyl-1-propanesulfonic acid-co-ethylene glycol dimethacrylate (Fe₃O₄/SiO₂/P(AMPS-co-EGDMA)) copolymer was prepared and used as a magnetic solid phase extraction (MSPE) medium for recovery of endogenous cytokinins (CKs) from plant extracts. This magnetic porous polymer was characterized by electron microscopy, nitrogen sorption experiments, elemental analysis and Fourier-transformed infrared spectroscopy. It was demonstrated to have high extraction capacity toward CKs in plants due to its specificity, surface area and porous structure. Coupled with hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS), a rapid, simple, and effective MSPE-HILIC-MS/MS analytical method for the quantitative analysis of endogenous CKs in Oryza sativa (O. sativa) roots was successfully established. Good linearities were obtained for all CKs investigated with correlation coefficients (R²>0.9975. The results showed that LODs (S/N=3) were ranged from 0.18 to 3.65 pg mL⁻¹. Reproducibility of the method was obtained with intra-day and inter-day relative standard deviations (RSDs) less than 16.1% and the recoveries in plant samples ranged from 72.8% to 115.5%. Finally, the MSPE-HILIC-MS/MS method was applied to several plant samples, and the amounts of endogenous CKs in O. sativa roots, leaves and Arabidopsis thaliana (A. thaliana) were successfully determined.

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Authors+Show Affiliations

Liu Z
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Cai BD
No affiliation info available
Feng YQ
No affiliation info available

MeSH

Chromatography, LiquidCytokininsMicroscopy, Electron, ScanningPlantsReproducibility of ResultsSolid Phase ExtractionSpectroscopy, Fourier Transform InfraredTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

22401906

Citation

Liu, Zhao, et al. "Rapid Determination of Endogenous Cytokinins in Plant Samples By Combination of Magnetic Solid Phase Extraction With Hydrophilic Interaction Chromatography-tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 891-892, 2012, pp. 27-35.
Liu Z, Cai BD, Feng YQ. Rapid determination of endogenous cytokinins in plant samples by combination of magnetic solid phase extraction with hydrophilic interaction chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;891-892:27-35.
Liu, Z., Cai, B. D., & Feng, Y. Q. (2012). Rapid determination of endogenous cytokinins in plant samples by combination of magnetic solid phase extraction with hydrophilic interaction chromatography-tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 891-892, 27-35. https://doi.org/10.1016/j.jchromb.2012.02.015
Liu Z, Cai BD, Feng YQ. Rapid Determination of Endogenous Cytokinins in Plant Samples By Combination of Magnetic Solid Phase Extraction With Hydrophilic Interaction Chromatography-tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 1;891-892:27-35. PubMed PMID: 22401906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid determination of endogenous cytokinins in plant samples by combination of magnetic solid phase extraction with hydrophilic interaction chromatography-tandem mass spectrometry. AU - Liu,Zhao, AU - Cai,Bao-Dong, AU - Feng,Yu-Qi, Y1 - 2012/02/18/ PY - 2011/11/29/received PY - 2012/02/08/revised PY - 2012/02/12/accepted PY - 2012/3/10/entrez PY - 2012/3/10/pubmed PY - 2012/5/9/medline SP - 27 EP - 35 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 891-892 N2 - A 2-acrylamido-2-methyl-1-propanesulfonic acid-co-ethylene glycol dimethacrylate (Fe₃O₄/SiO₂/P(AMPS-co-EGDMA)) copolymer was prepared and used as a magnetic solid phase extraction (MSPE) medium for recovery of endogenous cytokinins (CKs) from plant extracts. This magnetic porous polymer was characterized by electron microscopy, nitrogen sorption experiments, elemental analysis and Fourier-transformed infrared spectroscopy. It was demonstrated to have high extraction capacity toward CKs in plants due to its specificity, surface area and porous structure. Coupled with hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS), a rapid, simple, and effective MSPE-HILIC-MS/MS analytical method for the quantitative analysis of endogenous CKs in Oryza sativa (O. sativa) roots was successfully established. Good linearities were obtained for all CKs investigated with correlation coefficients (R²>0.9975. The results showed that LODs (S/N=3) were ranged from 0.18 to 3.65 pg mL⁻¹. Reproducibility of the method was obtained with intra-day and inter-day relative standard deviations (RSDs) less than 16.1% and the recoveries in plant samples ranged from 72.8% to 115.5%. Finally, the MSPE-HILIC-MS/MS method was applied to several plant samples, and the amounts of endogenous CKs in O. sativa roots, leaves and Arabidopsis thaliana (A. thaliana) were successfully determined. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/22401906/Rapid_determination_of_endogenous_cytokinins_in_plant_samples_by_combination_of_magnetic_solid_phase_extraction_with_hydrophilic_interaction_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(12)00103-1 DB - PRIME DP - Unbound Medicine ER -
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