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Effect of α-thalassaemia on exercise-induced oxidative stress in sickle cell trait.
Acta Physiol (Oxf). 2012 Aug; 205(4):541-50.AP

Abstract

AIM

Alpha-thalassaemia is known to reduce intra-erythrocyte HbS (sickle haemoglobin) concentration in sickle cell trait (SCT) subjects. Because HbS was shown to increase oxidative stress, the purpose of this study was to assess the effects of the coexistence of α-thalassaemia and SCT on oxidative stress markers and nitric oxide (NO) metabolism after an acute physical exercise.

METHODS

Forty subjects (age: 23.5 ± 2.21 years), SCT carriers (HbAS) or healthy subjects (HbAA), with (-αT) or without (-NαT) an associated α-thalassaemia took part in the study. Plasma markers of oxidative stress [advanced oxidation protein products (AOPP), protein carbonyl, malondialdehyde (MDA) and nitrotyrosine], anti-oxidant defences and NO metabolism (NOx) were measured at rest (T(rest)), immediately following an incremental maximal exercise test (T(ex)) and during recovery (T(1h), T(2h) and T(24h)).

RESULTS

Malondialdehyde expressed as the percentage of changes from baseline was significantly higher in the HbAS-NαT compared with HbAS-αT during recovery (+36.3 ± 14.1% vs. -1.8 ± 13.2% at T(1h), P = 0.02; +36.6 ± 13.4% vs. -11.4 ± 12.5% at T(2h), P = 0.004 and +24.1 ± 12.3% vs. -14.4 ± 11.5% at T(24h), P = 0.02 in HbAS-NαT vs. HbAS-αT). Compared with HbAS-NαT, HbAS-αT had a higher NOx change from baseline at T(ex) (-23.4 ± 20.6% vs. +57.7 ± 19.3%, respectively; P = 0.005) and lower nitrotyrosine change from baseline at T(1h) (+7.2 ± 22.2% vs. +93.5%±29.3%, respectively; P = 0.04).

CONCLUSION

All these data suggest that the presence of α-thalassaemia may blunt the higher level of oxidative stress and the impaired bioavailability of NO observed in the SCT carriers.

Authors+Show Affiliations

Center of Research and Innovation on Sports (CRIS EA647), University Claude Bernard Lyon 1, University of Lyon, Lyon, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22409225

Citation

Faёs, C, et al. "Effect of Α-thalassaemia On Exercise-induced Oxidative Stress in Sickle Cell Trait." Acta Physiologica (Oxford, England), vol. 205, no. 4, 2012, pp. 541-50.
Faёs C, Martin C, Chirico EN, et al. Effect of α-thalassaemia on exercise-induced oxidative stress in sickle cell trait. Acta Physiol (Oxf). 2012;205(4):541-50.
Faёs, C., Martin, C., Chirico, E. N., Féasson, L., Oyonno-Enguelle, S., Dubouchaud, H., Francina, A., Thiriet, P., Pialoux, V., & Messonnier, L. (2012). Effect of α-thalassaemia on exercise-induced oxidative stress in sickle cell trait. Acta Physiologica (Oxford, England), 205(4), 541-50. https://doi.org/10.1111/j.1748-1716.2012.02434.x
Faёs C, et al. Effect of Α-thalassaemia On Exercise-induced Oxidative Stress in Sickle Cell Trait. Acta Physiol (Oxf). 2012;205(4):541-50. PubMed PMID: 22409225.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of α-thalassaemia on exercise-induced oxidative stress in sickle cell trait. AU - Faёs,C, AU - Martin,C, AU - Chirico,E N, AU - Féasson,L, AU - Oyonno-Enguelle,S, AU - Dubouchaud,H, AU - Francina,A, AU - Thiriet,P, AU - Pialoux,V, AU - Messonnier,L, Y1 - 2012/04/10/ PY - 2011/11/10/received PY - 2011/12/14/revised PY - 2012/03/01/revised PY - 2012/03/04/accepted PY - 2012/3/14/entrez PY - 2012/3/14/pubmed PY - 2012/12/10/medline SP - 541 EP - 50 JF - Acta physiologica (Oxford, England) JO - Acta Physiol (Oxf) VL - 205 IS - 4 N2 - AIM: Alpha-thalassaemia is known to reduce intra-erythrocyte HbS (sickle haemoglobin) concentration in sickle cell trait (SCT) subjects. Because HbS was shown to increase oxidative stress, the purpose of this study was to assess the effects of the coexistence of α-thalassaemia and SCT on oxidative stress markers and nitric oxide (NO) metabolism after an acute physical exercise. METHODS: Forty subjects (age: 23.5 ± 2.21 years), SCT carriers (HbAS) or healthy subjects (HbAA), with (-αT) or without (-NαT) an associated α-thalassaemia took part in the study. Plasma markers of oxidative stress [advanced oxidation protein products (AOPP), protein carbonyl, malondialdehyde (MDA) and nitrotyrosine], anti-oxidant defences and NO metabolism (NOx) were measured at rest (T(rest)), immediately following an incremental maximal exercise test (T(ex)) and during recovery (T(1h), T(2h) and T(24h)). RESULTS: Malondialdehyde expressed as the percentage of changes from baseline was significantly higher in the HbAS-NαT compared with HbAS-αT during recovery (+36.3 ± 14.1% vs. -1.8 ± 13.2% at T(1h), P = 0.02; +36.6 ± 13.4% vs. -11.4 ± 12.5% at T(2h), P = 0.004 and +24.1 ± 12.3% vs. -14.4 ± 11.5% at T(24h), P = 0.02 in HbAS-NαT vs. HbAS-αT). Compared with HbAS-NαT, HbAS-αT had a higher NOx change from baseline at T(ex) (-23.4 ± 20.6% vs. +57.7 ± 19.3%, respectively; P = 0.005) and lower nitrotyrosine change from baseline at T(1h) (+7.2 ± 22.2% vs. +93.5%±29.3%, respectively; P = 0.04). CONCLUSION: All these data suggest that the presence of α-thalassaemia may blunt the higher level of oxidative stress and the impaired bioavailability of NO observed in the SCT carriers. SN - 1748-1716 UR - https://www.unboundmedicine.com/medline/citation/22409225/Effect_of_α_thalassaemia_on_exercise_induced_oxidative_stress_in_sickle_cell_trait_ L2 - https://doi.org/10.1111/j.1748-1716.2012.02434.x DB - PRIME DP - Unbound Medicine ER -