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Profiling of phenolic compounds and antioxidant activity of dry extracts from the selected Sorbus species.
Molecules 2012; 17(3):3093-113M

Abstract

The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and Trolox. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC(50) = 3.2-5.2 μg/mL), TEAC (2.8-4.0 mmol Trolox/g), and FRAP (9.8-13.7 mmol Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6-58.2% of gallic acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-β-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC(50) = 36.9-228.3 μg/mL) depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products.

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, Muszyńskiego 1, 90 151 Lodz, Poland. monika.olszewska@umed.lodz.plNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22410419

Citation

Olszewska, Monika A., et al. "Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts From the Selected Sorbus Species." Molecules (Basel, Switzerland), vol. 17, no. 3, 2012, pp. 3093-113.
Olszewska MA, Presler A, Michel P. Profiling of phenolic compounds and antioxidant activity of dry extracts from the selected Sorbus species. Molecules. 2012;17(3):3093-113.
Olszewska, M. A., Presler, A., & Michel, P. (2012). Profiling of phenolic compounds and antioxidant activity of dry extracts from the selected Sorbus species. Molecules (Basel, Switzerland), 17(3), pp. 3093-113. doi:10.3390/molecules17033093.
Olszewska MA, Presler A, Michel P. Profiling of Phenolic Compounds and Antioxidant Activity of Dry Extracts From the Selected Sorbus Species. Molecules. 2012 Mar 12;17(3):3093-113. PubMed PMID: 22410419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Profiling of phenolic compounds and antioxidant activity of dry extracts from the selected Sorbus species. AU - Olszewska,Monika A, AU - Presler,Anna, AU - Michel,Piotr, Y1 - 2012/03/12/ PY - 2012/02/15/received PY - 2012/03/05/revised PY - 2012/03/06/accepted PY - 2012/3/14/entrez PY - 2012/3/14/pubmed PY - 2012/7/4/medline SP - 3093 EP - 113 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 17 IS - 3 N2 - The antioxidant efficiency of dry extracts from inflorescences and/or leaves of seven Sorbus species was studied using four in vitro tests of SET (single electron transfer) and HAT-type (hydrogen atom transfer) mechanisms. The 70% methanol extracts and its diethyl ether, ethyl acetate, n-butanol and water fractions were tested in parallel with the phenolic standards, e.g., caffeic acid, quercetin, BHA, BHT, and Trolox. The SET-type activity of the extracts depended primarily on the extraction solvent. The most valuable extracts were n-butanol and ethyl acetate ones, which activity was high in the DPPH (EC(50) = 3.2-5.2 μg/mL), TEAC (2.8-4.0 mmol Trolox/g), and FRAP (9.8-13.7 mmol Fe2+/g) tests, and strongly correlated with the total phenolic levels (39.6-58.2% of gallic acid equivalents). The HPLC-PDA analysis of the extracts led to the identification of chlorogenic acid, isoquercitrin, hyperoside, rutin, quercetin 3-O-sophoroside, and sexangularetin 3-O-β-D-glucopyranoside as the main components. Apart from flavonoids and hydroxycinnamic acids, proanthocyanidins have also a significant impact on the SET-type activity. The HAT-reactivity of the extracts in the linoleic acid peroxidation test (IC(50) = 36.9-228.3 μg/mL) depended more strongly on the plant tissue than on the extraction solvent, and its correlation with the phenolic content was weak. Both SET and HAT-type activity of the most potent Sorbus extracts was comparable with the activity of the standards, indicating their great potential as effective sources for health products. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/22410419/Profiling_of_phenolic_compounds_and_antioxidant_activity_of_dry_extracts_from_the_selected_Sorbus_species_ L2 - http://www.mdpi.com/resolver?pii=molecules17033093 DB - PRIME DP - Unbound Medicine ER -