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Site-specific circadian expression of leptin and its receptor in human adipose tissue.
Nutr Hosp. 2011 Nov-Dec; 26(6):1394-401.NH

Abstract

INTRODUCTION

Circadian variability of circulating leptin levels has been well established over the last decade. However, the circadian behavior of leptin in human adipose tissue remains unknown. This also applies to the soluble leptin receptor.

OBJECTIVE

We investigated the ex vivo circadian behavior of leptin and its receptor expression in human adipose tissue (AT).

SUBJECTS AND METHODS

Visceral and subcutaneous abdominal AT biopsies (n = 6) were obtained from morbid obese women (BMI ≥ 40 kg/m²). Anthropometric variables and fasting plasma glucose, leptin, lipids and lipoprotein concentrations were determined. In order to investigate rhythmic expression pattern of leptin and its receptor, AT explants were cultured during 24-h and gene expression was analyzed at the following times: 08:00, 14:00, 20:00, 02:00 h, using quantitative real-time PCR.

RESULTS

Leptin expression showed an oscillatory pattern that was consistent with circadian rhythm in cultured AT. Similar patterns were noted for the leptin receptor. Leptin showed its achrophase (maximum expression) during the night, which might be associated to a lower degree of fat accumulation and higher mobilization. When comparing both fat depots, visceral AT anticipated its expression towards afternoon and evening hours. Interestingly, leptin plasma values were associated with decreased amplitude of LEP rhythm. This association was lost when adjusting for waist circumference.

CONCLUSION

Circadian rhythmicity has been demonstrated in leptin and its receptor in human AT cultures in a site-specific manner. This new knowledge paves the way for a better understanding of the autocrine/paracrine role of leptin in human AT.

Authors+Show Affiliations

Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22411388

Citation

Gómez Abellán, P, et al. "Site-specific Circadian Expression of Leptin and Its Receptor in Human Adipose Tissue." Nutricion Hospitalaria, vol. 26, no. 6, 2011, pp. 1394-401.
Gómez Abellán P, Gómez Santos C, Madrid JA, et al. Site-specific circadian expression of leptin and its receptor in human adipose tissue. Nutr Hosp. 2011;26(6):1394-401.
Gómez Abellán, P., Gómez Santos, C., Madrid, J. A., Milagro, F. I., Campion, J., Martínez, J. A., Luján, J. A., Ordovás, J. M., & Garaulet, M. (2011). Site-specific circadian expression of leptin and its receptor in human adipose tissue. Nutricion Hospitalaria, 26(6), 1394-401. https://doi.org/10.1590/S0212-16112011000600029
Gómez Abellán P, et al. Site-specific Circadian Expression of Leptin and Its Receptor in Human Adipose Tissue. Nutr Hosp. 2011 Nov-Dec;26(6):1394-401. PubMed PMID: 22411388.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Site-specific circadian expression of leptin and its receptor in human adipose tissue. AU - Gómez Abellán,P, AU - Gómez Santos,C, AU - Madrid,J A, AU - Milagro,F I, AU - Campion,J, AU - Martínez,J A, AU - Luján,J A, AU - Ordovás,J Ma, AU - Garaulet,M, PY - 2011/05/10/received PY - 2011/06/02/accepted PY - 2012/3/14/entrez PY - 2012/3/14/pubmed PY - 2012/7/4/medline SP - 1394 EP - 401 JF - Nutricion hospitalaria JO - Nutr Hosp VL - 26 IS - 6 N2 - INTRODUCTION: Circadian variability of circulating leptin levels has been well established over the last decade. However, the circadian behavior of leptin in human adipose tissue remains unknown. This also applies to the soluble leptin receptor. OBJECTIVE: We investigated the ex vivo circadian behavior of leptin and its receptor expression in human adipose tissue (AT). SUBJECTS AND METHODS: Visceral and subcutaneous abdominal AT biopsies (n = 6) were obtained from morbid obese women (BMI ≥ 40 kg/m²). Anthropometric variables and fasting plasma glucose, leptin, lipids and lipoprotein concentrations were determined. In order to investigate rhythmic expression pattern of leptin and its receptor, AT explants were cultured during 24-h and gene expression was analyzed at the following times: 08:00, 14:00, 20:00, 02:00 h, using quantitative real-time PCR. RESULTS: Leptin expression showed an oscillatory pattern that was consistent with circadian rhythm in cultured AT. Similar patterns were noted for the leptin receptor. Leptin showed its achrophase (maximum expression) during the night, which might be associated to a lower degree of fat accumulation and higher mobilization. When comparing both fat depots, visceral AT anticipated its expression towards afternoon and evening hours. Interestingly, leptin plasma values were associated with decreased amplitude of LEP rhythm. This association was lost when adjusting for waist circumference. CONCLUSION: Circadian rhythmicity has been demonstrated in leptin and its receptor in human AT cultures in a site-specific manner. This new knowledge paves the way for a better understanding of the autocrine/paracrine role of leptin in human AT. SN - 1699-5198 UR - https://www.unboundmedicine.com/medline/citation/22411388/Site_specific_circadian_expression_of_leptin_and_its_receptor_in_human_adipose_tissue_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22411388/ DB - PRIME DP - Unbound Medicine ER -