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Comparative study on protein cross-linking and gel enhancing effect of microbial transglutaminase on surimi from different fish.
J Sci Food Agric. 2012 Mar 15; 92(4):844-52.JS

Abstract

BACKGROUND

Microbial transglutaminase (MTGase) has been used to increase the gel strength of surimi. Nevertheless, its effectiveness varies with fish species. The aim of this study was to elucidate the effect of MTGase at different levels on protein cross-linking and gel property of surimi from threadfin bream, Indian mackerel and sardine in the presence and absence of endogenous transglutaminase.

RESULT

Breaking force of all surimi gels increased as MTGase levels (0-0.6 U g⁻¹) increased except for threadfin bream surimi gel, where the breaking force decreased at 0.6 U g⁻¹ (P < 0.05). In the presence of EDTA, the gel strengthening effect was lower, suggesting the combined effect of endogenous transglutaminase with MTGase. With the addition of MTGase, the gel with the highest increase in breaking force showed highest decrease in myosin heavy chain. When cross-linking activity of MTGase on natural actomyosin (NAM) was determined, the highest decreasing rate in ε-amino group content with the concomitant increased formation of cross-linked proteins was found in NAM from threadfin bream. The reactivity of muscle proteins toward MTGase-induced cross-linking was in agreement with surimi gel strengthening.

CONCLUSION

The composition and properties of muscle proteins of varying fish species more likely determined protein cross-linking induced by MTGase, thereby affecting their gel properties.

Authors+Show Affiliations

Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, Thailand.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22413145

Citation

Chanarat, Sochaya, et al. "Comparative Study On Protein Cross-linking and Gel Enhancing Effect of Microbial Transglutaminase On Surimi From Different Fish." Journal of the Science of Food and Agriculture, vol. 92, no. 4, 2012, pp. 844-52.
Chanarat S, Benjakul S, H-Kittikun A. Comparative study on protein cross-linking and gel enhancing effect of microbial transglutaminase on surimi from different fish. J Sci Food Agric. 2012;92(4):844-52.
Chanarat, S., Benjakul, S., & H-Kittikun, A. (2012). Comparative study on protein cross-linking and gel enhancing effect of microbial transglutaminase on surimi from different fish. Journal of the Science of Food and Agriculture, 92(4), 844-52.
Chanarat S, Benjakul S, H-Kittikun A. Comparative Study On Protein Cross-linking and Gel Enhancing Effect of Microbial Transglutaminase On Surimi From Different Fish. J Sci Food Agric. 2012 Mar 15;92(4):844-52. PubMed PMID: 22413145.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative study on protein cross-linking and gel enhancing effect of microbial transglutaminase on surimi from different fish. AU - Chanarat,Sochaya, AU - Benjakul,Soottawat, AU - H-Kittikun,Aran, PY - 2012/3/14/entrez PY - 2012/3/14/pubmed PY - 2012/6/22/medline SP - 844 EP - 52 JF - Journal of the science of food and agriculture JO - J Sci Food Agric VL - 92 IS - 4 N2 - BACKGROUND: Microbial transglutaminase (MTGase) has been used to increase the gel strength of surimi. Nevertheless, its effectiveness varies with fish species. The aim of this study was to elucidate the effect of MTGase at different levels on protein cross-linking and gel property of surimi from threadfin bream, Indian mackerel and sardine in the presence and absence of endogenous transglutaminase. RESULT: Breaking force of all surimi gels increased as MTGase levels (0-0.6 U g⁻¹) increased except for threadfin bream surimi gel, where the breaking force decreased at 0.6 U g⁻¹ (P < 0.05). In the presence of EDTA, the gel strengthening effect was lower, suggesting the combined effect of endogenous transglutaminase with MTGase. With the addition of MTGase, the gel with the highest increase in breaking force showed highest decrease in myosin heavy chain. When cross-linking activity of MTGase on natural actomyosin (NAM) was determined, the highest decreasing rate in ε-amino group content with the concomitant increased formation of cross-linked proteins was found in NAM from threadfin bream. The reactivity of muscle proteins toward MTGase-induced cross-linking was in agreement with surimi gel strengthening. CONCLUSION: The composition and properties of muscle proteins of varying fish species more likely determined protein cross-linking induced by MTGase, thereby affecting their gel properties. SN - 1097-0010 UR - https://www.unboundmedicine.com/medline/citation/22413145/Comparative_study_on_protein_cross_linking_and_gel_enhancing_effect_of_microbial_transglutaminase_on_surimi_from_different_fish_ L2 - https://doi.org/10.1002/jsfa.4656 DB - PRIME DP - Unbound Medicine ER -