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Proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid and serum of patients with rheumatoid arthritis and patients with chronic periodontitis.

Abstract

BACKGROUND

The aim of this study is to evaluate proinflammatory and anti-inflammatory cytokine levels in gingival crevicular fluid (GCF) and serum of rheumatoid arthritis (RA) and chronic periodontitis (CP) patients to assess whether cytokine profiles distinguish patients with RA and patients with CP while using healthy patients as background controls.

METHODS

A total of 49 patients, 17 patients with RA (three males and 14 females; mean age: 47.82 ± 10.74 years), 16 patients with CP (10 males and six females; mean age: 44.00 ± 7.00 years), and 16 controls (eight males and eight females; mean age: 28.06 ± 6.18 years) were enrolled. Patients with RA were under the supervision of rheumatologists; 15 of the patients with RA were being treated with methotrexate-sulfasalazine combined therapy, and two of the patients were being treated with leflunomid therapy. Periodontal parameters (plaque index, gingival index, probing depth, and clinical attachment level) were recorded. Interleukin (IL)-1β, IL-4, IL-10, and tumor necrosis factor-α (TNF-α) were determined in GCF and IL-1β and IL-10 in serum by enzyme-linked immunosorbent assay.

RESULTS

There were significant differences found among RA, CP, and control groups for all periodontal parameters (P <0.05). The total amount and concentration of GCF IL-1 β, IL-4, IL-10, and TNF-α were similar in RA and CP patients (P >0.05). Although the total amount and concentration of serum IL-10 was not significantly different among the groups (P >0.05), serum IL-1β was significantly lower in the RA group compared to CP patients and controls and was higher in GCF of the RA group compared to the CP group.

CONCLUSIONS

Although clinical periodontal disease parameters indicated more severe periodontal disease in CP compared to RA patients, immunologic evaluation did not reveal consistent results regarding proinflammatory and anti-inflammatory cytokine levels. This might be a result of the use of non-steroidal anti-inflammatory drugs and rheumatoid agents by patients with RA.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Periodontology, Faculty of Dentistry, Baskent University, Ankara, Turkey.

    , ,

    Source

    Journal of periodontology 84:1 2013 Jan pg 84-93

    MeSH

    Adult
    Aged
    Anti-Inflammatory Agents, Non-Steroidal
    Arthritis, Rheumatoid
    Chronic Periodontitis
    Cross-Sectional Studies
    Cytokines
    Dental Plaque Index
    Female
    Gingival Crevicular Fluid
    Humans
    Immunosuppressive Agents
    Inflammation Mediators
    Interleukin-10
    Interleukin-1beta
    Interleukin-4
    Male
    Methotrexate
    Middle Aged
    Periodontal Attachment Loss
    Periodontal Index
    Periodontal Pocket
    Sulfasalazine
    Tumor Necrosis Factor-alpha
    Young Adult

    Pub Type(s)

    Comparative Study
    Journal Article

    Language

    eng

    PubMed ID

    22414257

    Citation

    Cetinkaya, Burcu, et al. "Proinflammatory and Anti-inflammatory Cytokines in Gingival Crevicular Fluid and Serum of Patients With Rheumatoid Arthritis and Patients With Chronic Periodontitis." Journal of Periodontology, vol. 84, no. 1, 2013, pp. 84-93.
    Cetinkaya B, Guzeldemir E, Ogus E, et al. Proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid and serum of patients with rheumatoid arthritis and patients with chronic periodontitis. J Periodontol. 2013;84(1):84-93.
    Cetinkaya, B., Guzeldemir, E., Ogus, E., & Bulut, S. (2013). Proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid and serum of patients with rheumatoid arthritis and patients with chronic periodontitis. Journal of Periodontology, 84(1), pp. 84-93. doi:10.1902/jop.2012.110467.
    Cetinkaya B, et al. Proinflammatory and Anti-inflammatory Cytokines in Gingival Crevicular Fluid and Serum of Patients With Rheumatoid Arthritis and Patients With Chronic Periodontitis. J Periodontol. 2013;84(1):84-93. PubMed PMID: 22414257.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Proinflammatory and anti-inflammatory cytokines in gingival crevicular fluid and serum of patients with rheumatoid arthritis and patients with chronic periodontitis. AU - Cetinkaya,Burcu, AU - Guzeldemir,Esra, AU - Ogus,Ersin, AU - Bulut,Sule, Y1 - 2012/03/13/ PY - 2012/3/15/entrez PY - 2012/3/15/pubmed PY - 2013/6/15/medline SP - 84 EP - 93 JF - Journal of periodontology JO - J. Periodontol. VL - 84 IS - 1 N2 - BACKGROUND: The aim of this study is to evaluate proinflammatory and anti-inflammatory cytokine levels in gingival crevicular fluid (GCF) and serum of rheumatoid arthritis (RA) and chronic periodontitis (CP) patients to assess whether cytokine profiles distinguish patients with RA and patients with CP while using healthy patients as background controls. METHODS: A total of 49 patients, 17 patients with RA (three males and 14 females; mean age: 47.82 ± 10.74 years), 16 patients with CP (10 males and six females; mean age: 44.00 ± 7.00 years), and 16 controls (eight males and eight females; mean age: 28.06 ± 6.18 years) were enrolled. Patients with RA were under the supervision of rheumatologists; 15 of the patients with RA were being treated with methotrexate-sulfasalazine combined therapy, and two of the patients were being treated with leflunomid therapy. Periodontal parameters (plaque index, gingival index, probing depth, and clinical attachment level) were recorded. Interleukin (IL)-1β, IL-4, IL-10, and tumor necrosis factor-α (TNF-α) were determined in GCF and IL-1β and IL-10 in serum by enzyme-linked immunosorbent assay. RESULTS: There were significant differences found among RA, CP, and control groups for all periodontal parameters (P <0.05). The total amount and concentration of GCF IL-1 β, IL-4, IL-10, and TNF-α were similar in RA and CP patients (P >0.05). Although the total amount and concentration of serum IL-10 was not significantly different among the groups (P >0.05), serum IL-1β was significantly lower in the RA group compared to CP patients and controls and was higher in GCF of the RA group compared to the CP group. CONCLUSIONS: Although clinical periodontal disease parameters indicated more severe periodontal disease in CP compared to RA patients, immunologic evaluation did not reveal consistent results regarding proinflammatory and anti-inflammatory cytokine levels. This might be a result of the use of non-steroidal anti-inflammatory drugs and rheumatoid agents by patients with RA. SN - 1943-3670 UR - https://www.unboundmedicine.com/medline/citation/22414257/Proinflammatory_and_anti_inflammatory_cytokines_in_gingival_crevicular_fluid_and_serum_of_patients_with_rheumatoid_arthritis_and_patients_with_chronic_periodontitis_ L2 - https://doi.org/10.1902/jop.2012.110467 DB - PRIME DP - Unbound Medicine ER -