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Danggui-Shaoyao-San ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in d-galactose-induced senescent mice.
J Ethnopharmacol. 2012 May 07; 141(1):386-95.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Danggui-Shaoyao-San (DSS), a famous traditional Chinese medicine formula consisting of six herbal medicines, has been used to treat gynecological disorders and neural dysfunctions.

AIM OF THE STUDY

The present study was carried out to investigate the effects of DSS on cognitive ability and oxidative stress-related neuronal apoptosis in the hippocampus of aging mice induced by d-galactose (d-gal) to elucidate the underlying molecular mechanisms.

MATERIALS AND METHODS

Ethanol extract of DSS (DE) were orally administered to d-gal-induced senescent mice for six weeks. The cognitive ability was determined by the methods of step-down type passive avoidance test and Morris water maze test. The activities of superoxide dismutase (SOD) and nitric oxide synthase (NOS), and levels of carbonyl protein (CP), glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) were also examined. Furthermore, the expression of apoptotic related proteins in hippocampus of d-gal-treated mice, such as Bcl-2, Bax and caspase-3 proteins, were determined by immunohistochemistry.

RESULTS

DE at the doses of 1.8, 3.6 and 7.2g/kg significantly enhanced the cognitive performances and restored the abnormal activities of SOD and NOS and levels of CP, MDA, GSH and NO induced by d-gal. Moreover, the neural apoptosis in the hippocampus of d-gal-treated mice was improved by DE through regulating the expression of Bcl-2, Bax and caspase-3.

CONCLUSION

These results demonstrate that DE has neuroprotective effects in d-gal-induced senescent mice by ameliorating oxidative stress induced neuronal apoptosis in the brain.

Authors+Show Affiliations

Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22414474

Citation

Lan, Zhou, et al. "Danggui-Shaoyao-San Ameliorates Cognition Deficits and Attenuates Oxidative Stress-related Neuronal Apoptosis in D-galactose-induced Senescent Mice." Journal of Ethnopharmacology, vol. 141, no. 1, 2012, pp. 386-95.
Lan Z, Liu J, Chen L, et al. Danggui-Shaoyao-San ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in d-galactose-induced senescent mice. J Ethnopharmacol. 2012;141(1):386-95.
Lan, Z., Liu, J., Chen, L., Fu, Q., Luo, J., Qu, R., Kong, L., & Ma, S. (2012). Danggui-Shaoyao-San ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in d-galactose-induced senescent mice. Journal of Ethnopharmacology, 141(1), 386-95. https://doi.org/10.1016/j.jep.2012.02.050
Lan Z, et al. Danggui-Shaoyao-San Ameliorates Cognition Deficits and Attenuates Oxidative Stress-related Neuronal Apoptosis in D-galactose-induced Senescent Mice. J Ethnopharmacol. 2012 May 7;141(1):386-95. PubMed PMID: 22414474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Danggui-Shaoyao-San ameliorates cognition deficits and attenuates oxidative stress-related neuronal apoptosis in d-galactose-induced senescent mice. AU - Lan,Zhou, AU - Liu,Jiping, AU - Chen,Lvyi, AU - Fu,Qiang, AU - Luo,Jianguang, AU - Qu,Rong, AU - Kong,Lingyi, AU - Ma,Shiping, Y1 - 2012/03/05/ PY - 2011/11/10/received PY - 2012/02/21/revised PY - 2012/02/27/accepted PY - 2012/3/15/entrez PY - 2012/3/15/pubmed PY - 2012/8/11/medline SP - 386 EP - 95 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Danggui-Shaoyao-San (DSS), a famous traditional Chinese medicine formula consisting of six herbal medicines, has been used to treat gynecological disorders and neural dysfunctions. AIM OF THE STUDY: The present study was carried out to investigate the effects of DSS on cognitive ability and oxidative stress-related neuronal apoptosis in the hippocampus of aging mice induced by d-galactose (d-gal) to elucidate the underlying molecular mechanisms. MATERIALS AND METHODS: Ethanol extract of DSS (DE) were orally administered to d-gal-induced senescent mice for six weeks. The cognitive ability was determined by the methods of step-down type passive avoidance test and Morris water maze test. The activities of superoxide dismutase (SOD) and nitric oxide synthase (NOS), and levels of carbonyl protein (CP), glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) were also examined. Furthermore, the expression of apoptotic related proteins in hippocampus of d-gal-treated mice, such as Bcl-2, Bax and caspase-3 proteins, were determined by immunohistochemistry. RESULTS: DE at the doses of 1.8, 3.6 and 7.2g/kg significantly enhanced the cognitive performances and restored the abnormal activities of SOD and NOS and levels of CP, MDA, GSH and NO induced by d-gal. Moreover, the neural apoptosis in the hippocampus of d-gal-treated mice was improved by DE through regulating the expression of Bcl-2, Bax and caspase-3. CONCLUSION: These results demonstrate that DE has neuroprotective effects in d-gal-induced senescent mice by ameliorating oxidative stress induced neuronal apoptosis in the brain. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22414474/Danggui_Shaoyao_San_ameliorates_cognition_deficits_and_attenuates_oxidative_stress_related_neuronal_apoptosis_in_d_galactose_induced_senescent_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00146-8 DB - PRIME DP - Unbound Medicine ER -