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The effects of epidermal growth factor and transforming growth factor-α on secretion of interleukin-8 and growth-regulated oncogene-α in human granulosa-lutein cells.
Gynecol Obstet Invest. 2012; 73(3):189-94.GO

Abstract

BACKGROUND

In order to investigate the roles of epidermal growth factor (EGF) and transforming growth factor (TGF)-α in ovulation, we studied the production of interleukin (IL)-8 and growth-regulated oncogene (GRO)-α in cultured human granulosa-lutein cells.

METHODS

Granulosa-lutein cells obtained from the follicular fluids of in vitro fertilization and embryo transfer patients were cultured and treated with EGF, TGF-α, tumor necrosis factor (TNF)-α or 12-O-tetradecanoylphorbol 13-acetate (TPA). An immortalized granulosa cell line (GC1a) was also cultured and treated with EGF, TGF-α or mitogen-activated protein kinase kinase inhibitor. The supernatants were collected, and IL-8 and GRO-α were measured by ELISA.

RESULTS

The levels of IL-8 and GRO-α were significantly increased after treatment with EGF, TGF-α, TNF-α and TPA by primary cultured granulosa-lutein cells. The levels of IL-8 and GRO-α were also significantly increased after treatment with EGF or TGF-α in a dose-dependent manner by GC1a. When GC1a was treated with EGF, TGF-α or U0126, the levels of IL-8 and GRO-α were significantly decreased.

CONCLUSION

Our data indicate that the production of IL-8 and GRO-α is upregulated by EGF and TGF-α. It is suggested that EGF and TGF-α may play an important role in luteinization processes involving IL-8 and GRO-α production.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita, Japan. kawayas@oita-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22414682

Citation

Kawano, Yasushi, et al. "The Effects of Epidermal Growth Factor and Transforming Growth Factor-α On Secretion of Interleukin-8 and Growth-regulated Oncogene-α in Human Granulosa-lutein Cells." Gynecologic and Obstetric Investigation, vol. 73, no. 3, 2012, pp. 189-94.
Kawano Y, Zeineh K, Furukawa Y, et al. The effects of epidermal growth factor and transforming growth factor-α on secretion of interleukin-8 and growth-regulated oncogene-α in human granulosa-lutein cells. Gynecol Obstet Invest. 2012;73(3):189-94.
Kawano, Y., Zeineh, K., Furukawa, Y., Utsunomiya, Y., Okamoto, M., & Narahara, H. (2012). The effects of epidermal growth factor and transforming growth factor-α on secretion of interleukin-8 and growth-regulated oncogene-α in human granulosa-lutein cells. Gynecologic and Obstetric Investigation, 73(3), 189-94. https://doi.org/10.1159/000331665
Kawano Y, et al. The Effects of Epidermal Growth Factor and Transforming Growth Factor-α On Secretion of Interleukin-8 and Growth-regulated Oncogene-α in Human Granulosa-lutein Cells. Gynecol Obstet Invest. 2012;73(3):189-94. PubMed PMID: 22414682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of epidermal growth factor and transforming growth factor-α on secretion of interleukin-8 and growth-regulated oncogene-α in human granulosa-lutein cells. AU - Kawano,Yasushi, AU - Zeineh,Kosay, AU - Furukawa,Yuichi, AU - Utsunomiya,Yufuko, AU - Okamoto,Mamiko, AU - Narahara,Hisashi, Y1 - 2012/03/13/ PY - 2011/01/25/received PY - 2011/07/20/accepted PY - 2012/3/15/entrez PY - 2012/3/15/pubmed PY - 2012/8/22/medline SP - 189 EP - 94 JF - Gynecologic and obstetric investigation JO - Gynecol Obstet Invest VL - 73 IS - 3 N2 - BACKGROUND: In order to investigate the roles of epidermal growth factor (EGF) and transforming growth factor (TGF)-α in ovulation, we studied the production of interleukin (IL)-8 and growth-regulated oncogene (GRO)-α in cultured human granulosa-lutein cells. METHODS: Granulosa-lutein cells obtained from the follicular fluids of in vitro fertilization and embryo transfer patients were cultured and treated with EGF, TGF-α, tumor necrosis factor (TNF)-α or 12-O-tetradecanoylphorbol 13-acetate (TPA). An immortalized granulosa cell line (GC1a) was also cultured and treated with EGF, TGF-α or mitogen-activated protein kinase kinase inhibitor. The supernatants were collected, and IL-8 and GRO-α were measured by ELISA. RESULTS: The levels of IL-8 and GRO-α were significantly increased after treatment with EGF, TGF-α, TNF-α and TPA by primary cultured granulosa-lutein cells. The levels of IL-8 and GRO-α were also significantly increased after treatment with EGF or TGF-α in a dose-dependent manner by GC1a. When GC1a was treated with EGF, TGF-α or U0126, the levels of IL-8 and GRO-α were significantly decreased. CONCLUSION: Our data indicate that the production of IL-8 and GRO-α is upregulated by EGF and TGF-α. It is suggested that EGF and TGF-α may play an important role in luteinization processes involving IL-8 and GRO-α production. SN - 1423-002X UR - https://www.unboundmedicine.com/medline/citation/22414682/The_effects_of_epidermal_growth_factor_and_transforming_growth_factor_��_on_secretion_of_interleukin_8_and_growth_regulated_oncogene_��_in_human_granulosa_lutein_cells_ L2 - https://www.karger.com?DOI=10.1159/000331665 DB - PRIME DP - Unbound Medicine ER -