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Molecular characterization of wheat allergens specifically recognized by patients suffering from wheat-induced respiratory allergy.
Clin Exp Allergy 2012; 42(4):597-609CE

Abstract

BACKGROUND

Wheat (Triticum aestivum) is an important allergen source responsible for various clinical manifestations of allergy (i.e. food allergy, pollen allergy, respiratory allergy to flour-Baker's asthma).

OBJECTIVE

The objective of this study was the molecular and immunological characterization of new recombinant wheat allergens and to evaluate their usefulness for the diagnosis of allergy to wheat.

METHODS

A T. aestivum cDNA library was constructed and screened with serum IgE from patients suffering from wheat allergy to identify cDNAs coding for new wheat allergens. The allergen-encoding cDNAs were expressed in Escherichia coli and purified to homogeneity. IgE reactivity of recombinant proteins was analysed in RAST-based, non-denaturing dot blot experiments and by ELISA with sera from wheat allergic patients and their allergenic activity was assessed in basophil degranulation experiments.

RESULTS

We report the molecular characterization, recombinant expression and purification of five wheat allergens, a thioredoxin h isoform, glutathione transferase, 1-Cys-peroxiredoxin, profilin and dehydrin. Homologous proteins were identified by sequence comparisons in various plants. 1-Cys-peroxiredoxin appeared to be the most relevant of the newly identified wheat allergens according to prevalence of IgE recognition and results from basophil degranulation experiments. It showed IgE cross-reactivity with seed proteins from barley, rye, rice, maize, soy, oat and spelt. 1-Cys-peroxiredoxin, glutathione transferase and dehydrin were mainly recognized by patients with baker's asthma but not wheat-induced food allergy.

CONCLUSION AND CLINICAL RELEVANCE

The characterized recombinant wheat allergens may be useful for the development of serological tests which allow the discrimination of different clinical manifestations of wheat allergy.

Authors+Show Affiliations

Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna General Hospital, Waehringer Guertel 18-20, Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22417217

Citation

Pahr, S, et al. "Molecular Characterization of Wheat Allergens Specifically Recognized By Patients Suffering From Wheat-induced Respiratory Allergy." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 42, no. 4, 2012, pp. 597-609.
Pahr S, Constantin C, Mari A, et al. Molecular characterization of wheat allergens specifically recognized by patients suffering from wheat-induced respiratory allergy. Clin Exp Allergy. 2012;42(4):597-609.
Pahr, S., Constantin, C., Mari, A., Scheiblhofer, S., Thalhamer, J., Ebner, C., ... Valenta, R. (2012). Molecular characterization of wheat allergens specifically recognized by patients suffering from wheat-induced respiratory allergy. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 42(4), pp. 597-609. doi:10.1111/j.1365-2222.2012.03961.x.
Pahr S, et al. Molecular Characterization of Wheat Allergens Specifically Recognized By Patients Suffering From Wheat-induced Respiratory Allergy. Clin Exp Allergy. 2012;42(4):597-609. PubMed PMID: 22417217.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of wheat allergens specifically recognized by patients suffering from wheat-induced respiratory allergy. AU - Pahr,S, AU - Constantin,C, AU - Mari,A, AU - Scheiblhofer,S, AU - Thalhamer,J, AU - Ebner,C, AU - Vrtala,S, AU - Mittermann,I, AU - Valenta,R, PY - 2012/3/16/entrez PY - 2012/3/16/pubmed PY - 2012/6/29/medline SP - 597 EP - 609 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 42 IS - 4 N2 - BACKGROUND: Wheat (Triticum aestivum) is an important allergen source responsible for various clinical manifestations of allergy (i.e. food allergy, pollen allergy, respiratory allergy to flour-Baker's asthma). OBJECTIVE: The objective of this study was the molecular and immunological characterization of new recombinant wheat allergens and to evaluate their usefulness for the diagnosis of allergy to wheat. METHODS: A T. aestivum cDNA library was constructed and screened with serum IgE from patients suffering from wheat allergy to identify cDNAs coding for new wheat allergens. The allergen-encoding cDNAs were expressed in Escherichia coli and purified to homogeneity. IgE reactivity of recombinant proteins was analysed in RAST-based, non-denaturing dot blot experiments and by ELISA with sera from wheat allergic patients and their allergenic activity was assessed in basophil degranulation experiments. RESULTS: We report the molecular characterization, recombinant expression and purification of five wheat allergens, a thioredoxin h isoform, glutathione transferase, 1-Cys-peroxiredoxin, profilin and dehydrin. Homologous proteins were identified by sequence comparisons in various plants. 1-Cys-peroxiredoxin appeared to be the most relevant of the newly identified wheat allergens according to prevalence of IgE recognition and results from basophil degranulation experiments. It showed IgE cross-reactivity with seed proteins from barley, rye, rice, maize, soy, oat and spelt. 1-Cys-peroxiredoxin, glutathione transferase and dehydrin were mainly recognized by patients with baker's asthma but not wheat-induced food allergy. CONCLUSION AND CLINICAL RELEVANCE: The characterized recombinant wheat allergens may be useful for the development of serological tests which allow the discrimination of different clinical manifestations of wheat allergy. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/22417217/Molecular_characterization_of_wheat_allergens_specifically_recognized_by_patients_suffering_from_wheat_induced_respiratory_allergy_ L2 - https://doi.org/10.1111/j.1365-2222.2012.03961.x DB - PRIME DP - Unbound Medicine ER -