Tags

Type your tag names separated by a space and hit enter

Reactive oxygen species and mitogen-activated protein kinase induce apoptotic death of SH-SY5Y cells in response to fipronil.
Toxicol Lett. 2012 May 20; 211(1):18-28.TL

Abstract

There are multiple lines of evidence showing that environmental toxicants including pesticides may contribute to neuronal cell death. Fipronil (FPN) is a phenylpyrazole insecticide that acts on insect GABA receptors. Although the action of FPN is restricted to insect neuronal or muscular transmitter systems, a few studies have assessed the effects of this neurotoxicant on neuronal cell death distinct from an insect. To determine the mechanisms underlying FPN-induced neuronal cell death, we evaluated the ability of this chemical to induce oxidative stress and studied the involvement of mitogen activated protein kinases (MAPKs) in FPN-induced apoptosis stress in human neuroblastoma SH-SY5Y (SH-SY5Y) cells. Exposure of SH-SY5Y cells to FPN led to the production of reactive oxygen species (ROS) and apoptotic cell death via activation of caspase-9 and caspase-3. Interestingly, the antioxidant, N-acetyl-cysteine (NAC) attenuated apoptotic cell death and ROS production induced by FPN. These results indicated that oxidative stress plays a central role in FPN-induced cytotoxicity. Mitochondrial complex I activity was also inhibited by FPN treatment. These finding indicate that FPN triggers intrinsic apoptosis via the mitochondrial signaling pathway that is initiated by the generation of ROS. Furthermore, FPN treatment induced phosphorylation of MAPK members. Activation of these protein kinases by FPN was involved in the onset of apoptosis as inhibitors specific to these kinases protect against FPN-induced cell death as well as ROS generation. Our data indicate that FPN-induced apoptosis is mediated primarily by the generation of ROS and activation of MAPK members followed by activation of the intrinsic apoptotic pathway.

Authors+Show Affiliations

Department of Pharmacology, College of Medicine, Hanyang University, Sungdong-Gu, Heandang-Dong 17, 133-791 Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22421270

Citation

Ki, Yeo-Woon, et al. "Reactive Oxygen Species and Mitogen-activated Protein Kinase Induce Apoptotic Death of SH-SY5Y Cells in Response to Fipronil." Toxicology Letters, vol. 211, no. 1, 2012, pp. 18-28.
Ki YW, Lee JE, Park JH, et al. Reactive oxygen species and mitogen-activated protein kinase induce apoptotic death of SH-SY5Y cells in response to fipronil. Toxicol Lett. 2012;211(1):18-28.
Ki, Y. W., Lee, J. E., Park, J. H., Shin, I. C., & Koh, H. C. (2012). Reactive oxygen species and mitogen-activated protein kinase induce apoptotic death of SH-SY5Y cells in response to fipronil. Toxicology Letters, 211(1), 18-28. https://doi.org/10.1016/j.toxlet.2012.02.022
Ki YW, et al. Reactive Oxygen Species and Mitogen-activated Protein Kinase Induce Apoptotic Death of SH-SY5Y Cells in Response to Fipronil. Toxicol Lett. 2012 May 20;211(1):18-28. PubMed PMID: 22421270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reactive oxygen species and mitogen-activated protein kinase induce apoptotic death of SH-SY5Y cells in response to fipronil. AU - Ki,Yeo-Woon, AU - Lee,Jeong Eun, AU - Park,Jae Hyeon, AU - Shin,In Chul, AU - Koh,Hyun Chul, Y1 - 2012/03/07/ PY - 2011/12/21/received PY - 2012/02/24/revised PY - 2012/02/27/accepted PY - 2012/3/17/entrez PY - 2012/3/17/pubmed PY - 2012/6/30/medline SP - 18 EP - 28 JF - Toxicology letters JO - Toxicol Lett VL - 211 IS - 1 N2 - There are multiple lines of evidence showing that environmental toxicants including pesticides may contribute to neuronal cell death. Fipronil (FPN) is a phenylpyrazole insecticide that acts on insect GABA receptors. Although the action of FPN is restricted to insect neuronal or muscular transmitter systems, a few studies have assessed the effects of this neurotoxicant on neuronal cell death distinct from an insect. To determine the mechanisms underlying FPN-induced neuronal cell death, we evaluated the ability of this chemical to induce oxidative stress and studied the involvement of mitogen activated protein kinases (MAPKs) in FPN-induced apoptosis stress in human neuroblastoma SH-SY5Y (SH-SY5Y) cells. Exposure of SH-SY5Y cells to FPN led to the production of reactive oxygen species (ROS) and apoptotic cell death via activation of caspase-9 and caspase-3. Interestingly, the antioxidant, N-acetyl-cysteine (NAC) attenuated apoptotic cell death and ROS production induced by FPN. These results indicated that oxidative stress plays a central role in FPN-induced cytotoxicity. Mitochondrial complex I activity was also inhibited by FPN treatment. These finding indicate that FPN triggers intrinsic apoptosis via the mitochondrial signaling pathway that is initiated by the generation of ROS. Furthermore, FPN treatment induced phosphorylation of MAPK members. Activation of these protein kinases by FPN was involved in the onset of apoptosis as inhibitors specific to these kinases protect against FPN-induced cell death as well as ROS generation. Our data indicate that FPN-induced apoptosis is mediated primarily by the generation of ROS and activation of MAPK members followed by activation of the intrinsic apoptotic pathway. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/22421270/Reactive_oxygen_species_and_mitogen_activated_protein_kinase_induce_apoptotic_death_of_SH_SY5Y_cells_in_response_to_fipronil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(12)00078-1 DB - PRIME DP - Unbound Medicine ER -