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Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb.
J Ethnopharmacol. 2012 May 07; 141(1):432-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Calophyllum brasiliense Camb. (Clusiaceae), popularly known as 'guanandi', is found in the tropical areas and swampy lands. The latex exuding from its bark is used in the treatment of gastric ulcer in folk medicine. Several active compounds have been isolated from its stem bark among them, are the chromanone acids. Therefore, this study aimed to evaluate antiulcer activity and probable mechanism(s) of action of a fraction containing a mixture of chromanone acids (BI), derived by column chromatography fractionation of the hexane extract of the stem bark of Calophyllum brasiliense (HECb), using experimental in vitro and in vivo models.

MATERIALS AND METHODS

Ulcer was induced by oral administration of ethanol (75%, v/v) and indomethacin (50mg/kg). Malondialdehyde, reduced glutathione and catalase activity was measured in stomach tissue after ethanol induced ulcer. In order to evaluate the effect of BI on nitric oxide, ulcer was induced by ethanol in l-NAME pretreated animals. Anti-Helicobacter pylori activity was verified in disk diffusion and broth microdilution in vitro assays, using cagA+ and vacA+ Helicobacter pylori strains.

RESULTS

BI prevented the gastric ulceration caused by ethanol and indomethacin treatments. Its gastroprotective mechanism in ethanol-induced ulcer was partly due to reduction of MDA and CAT levels in the gastric tissue. BI did not affect the GSH levels and its gastroprotective effect was not reversed by pretreatment with l-NAME. BI showed anti-Helicobacter pylori in the both assays.

CONCLUSION

The results indicate that BI is partly responsible for the HECb antiulcer and anti-Helicobacter pylori effects.

Authors+Show Affiliations

Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa, n 2367, Coxipó, Cuiabá, Mato Grosso, Zip Code: 78060-900, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22425905

Citation

Lemos, Larissa Maria Scalon, et al. "Evaluation of Antiulcer Activity of Chromanone Fraction From Calophyllum Brasiliesnse Camb." Journal of Ethnopharmacology, vol. 141, no. 1, 2012, pp. 432-9.
Lemos LM, Martins TB, Tanajura GH, et al. Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb. J Ethnopharmacol. 2012;141(1):432-9.
Lemos, L. M., Martins, T. B., Tanajura, G. H., Gazoni, V. F., Bonaldo, J., Strada, C. L., Silva, M. G., Dall'oglio, E. L., de Sousa Júnior, P. T., & Martins, D. T. (2012). Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb. Journal of Ethnopharmacology, 141(1), 432-9. https://doi.org/10.1016/j.jep.2012.03.006
Lemos LM, et al. Evaluation of Antiulcer Activity of Chromanone Fraction From Calophyllum Brasiliesnse Camb. J Ethnopharmacol. 2012 May 7;141(1):432-9. PubMed PMID: 22425905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb. AU - Lemos,Larissa Maria Scalon, AU - Martins,Thaís Bezerra, AU - Tanajura,Guilherme Henrique, AU - Gazoni,Vanessa Fátima, AU - Bonaldo,Josiane, AU - Strada,Claudia Léia, AU - Silva,Marcos Gabriel da, AU - Dall'oglio,Evandro Luiz, AU - de Sousa Júnior,Paulo Teixeira, AU - Martins,Domingos Tabajara de Oliveira, Y1 - 2012/03/15/ PY - 2011/12/14/received PY - 2012/02/01/revised PY - 2012/03/05/accepted PY - 2012/3/20/entrez PY - 2012/3/20/pubmed PY - 2012/8/11/medline SP - 432 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Calophyllum brasiliense Camb. (Clusiaceae), popularly known as 'guanandi', is found in the tropical areas and swampy lands. The latex exuding from its bark is used in the treatment of gastric ulcer in folk medicine. Several active compounds have been isolated from its stem bark among them, are the chromanone acids. Therefore, this study aimed to evaluate antiulcer activity and probable mechanism(s) of action of a fraction containing a mixture of chromanone acids (BI), derived by column chromatography fractionation of the hexane extract of the stem bark of Calophyllum brasiliense (HECb), using experimental in vitro and in vivo models. MATERIALS AND METHODS: Ulcer was induced by oral administration of ethanol (75%, v/v) and indomethacin (50mg/kg). Malondialdehyde, reduced glutathione and catalase activity was measured in stomach tissue after ethanol induced ulcer. In order to evaluate the effect of BI on nitric oxide, ulcer was induced by ethanol in l-NAME pretreated animals. Anti-Helicobacter pylori activity was verified in disk diffusion and broth microdilution in vitro assays, using cagA+ and vacA+ Helicobacter pylori strains. RESULTS: BI prevented the gastric ulceration caused by ethanol and indomethacin treatments. Its gastroprotective mechanism in ethanol-induced ulcer was partly due to reduction of MDA and CAT levels in the gastric tissue. BI did not affect the GSH levels and its gastroprotective effect was not reversed by pretreatment with l-NAME. BI showed anti-Helicobacter pylori in the both assays. CONCLUSION: The results indicate that BI is partly responsible for the HECb antiulcer and anti-Helicobacter pylori effects. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22425905/Evaluation_of_antiulcer_activity_of_chromanone_fraction_from_Calophyllum_brasiliesnse_Camb_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00154-7 DB - PRIME DP - Unbound Medicine ER -