Tags

Type your tag names separated by a space and hit enter

Effects of hecogenin and its possible mechanism of action on experimental models of gastric ulcer in mice.
Eur J Pharmacol. 2012 May 15; 683(1-3):260-9.EJ

Abstract

This study investigates the gastroprotective effects of hecogenin, a steroid saponin isolated from Agave sisalana, on experimental models of gastric ulcer. Male Swiss mice were used in the models of ethanol- and indometacin-induced gastric ulcer. To clarify the hecogenin mechanism of action, the roles of nitric oxide (NO), sulfhydryls (GSH), K⁺(ATP) channels and prostaglandins were also investigated, and measurements of lipid peroxidation (TBARS assay) and nitrite levels in the stomach of hecogenin-treated and untreated animals were performed. Furthermore, the effects of hecogenin on myeloperoxidase (MPO) release from human neutrophils were assessed in vitro. Our results showed that hecogenin (3.1, 7.5, 15, 30, 60 and 90 mg/kg, p.o.) acutely administered, before ethanol or indomethacin, exhibited a potent gastroprotective effect. Although the pretreatments with L-NAME, an iNOS inhibitor, and capsazepine, a TRPV1 receptor agonist, were not able to reverse the hecogenin effect, this was reversed by glibenclamide, a K⁺(ATP) blocker, and indomethacin in the model of ethanol-induced gastric lesions. The hecogenin pretreatment normalized GSH levels and significantly reduced lipid peroxidation and nitrite levels in the stomach, as evaluated by the ethanol-induced gastric lesion model. The drug alone increased COX-2 expression and this effect was further enhanced in the presence of ethanol. It also decreased MPO release and significantly protected the gastric mucosa. In conclusion, we showed that hecogenin presents a significant gastroprotective effect that seems to be mediated by K⁺(ATP) channels opening and the COX-2/PG pathway. In addition, its antioxidant and anti-inflammatory properties may play a role in the gastroprotective drug effect.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1127, CEP 60.431-270, Fortaleza, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22426163

Citation

Santos Cerqueira, Gilberto, et al. "Effects of Hecogenin and Its Possible Mechanism of Action On Experimental Models of Gastric Ulcer in Mice." European Journal of Pharmacology, vol. 683, no. 1-3, 2012, pp. 260-9.
Santos Cerqueira G, dos Santos e Silva G, Rios Vasconcelos E, et al. Effects of hecogenin and its possible mechanism of action on experimental models of gastric ulcer in mice. Eur J Pharmacol. 2012;683(1-3):260-9.
Santos Cerqueira, G., dos Santos e Silva, G., Rios Vasconcelos, E., Fragoso de Freitas, A. P., Arcanjo Moura, B., Silveira Macedo, D., Lopes Souto, A., Barbosa Filho, J. M., de Almeida Leal, L. K., de Castro Brito, G. A., Souccar, C., & de Barros Viana, G. S. (2012). Effects of hecogenin and its possible mechanism of action on experimental models of gastric ulcer in mice. European Journal of Pharmacology, 683(1-3), 260-9. https://doi.org/10.1016/j.ejphar.2012.02.043
Santos Cerqueira G, et al. Effects of Hecogenin and Its Possible Mechanism of Action On Experimental Models of Gastric Ulcer in Mice. Eur J Pharmacol. 2012 May 15;683(1-3):260-9. PubMed PMID: 22426163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of hecogenin and its possible mechanism of action on experimental models of gastric ulcer in mice. AU - Santos Cerqueira,Gilberto, AU - dos Santos e Silva,Gabriela, AU - Rios Vasconcelos,Emiliano, AU - Fragoso de Freitas,Ana Paula, AU - Arcanjo Moura,Brinell, AU - Silveira Macedo,Danielle, AU - Lopes Souto,Augusto, AU - Barbosa Filho,José Maria, AU - de Almeida Leal,Luzia Kalyne, AU - de Castro Brito,Gerly Anne, AU - Souccar,Caden, AU - de Barros Viana,Glauce Socorro, Y1 - 2012/03/08/ PY - 2011/02/21/received PY - 2012/02/18/revised PY - 2012/02/26/accepted PY - 2012/3/20/entrez PY - 2012/3/20/pubmed PY - 2012/9/14/medline SP - 260 EP - 9 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 683 IS - 1-3 N2 - This study investigates the gastroprotective effects of hecogenin, a steroid saponin isolated from Agave sisalana, on experimental models of gastric ulcer. Male Swiss mice were used in the models of ethanol- and indometacin-induced gastric ulcer. To clarify the hecogenin mechanism of action, the roles of nitric oxide (NO), sulfhydryls (GSH), K⁺(ATP) channels and prostaglandins were also investigated, and measurements of lipid peroxidation (TBARS assay) and nitrite levels in the stomach of hecogenin-treated and untreated animals were performed. Furthermore, the effects of hecogenin on myeloperoxidase (MPO) release from human neutrophils were assessed in vitro. Our results showed that hecogenin (3.1, 7.5, 15, 30, 60 and 90 mg/kg, p.o.) acutely administered, before ethanol or indomethacin, exhibited a potent gastroprotective effect. Although the pretreatments with L-NAME, an iNOS inhibitor, and capsazepine, a TRPV1 receptor agonist, were not able to reverse the hecogenin effect, this was reversed by glibenclamide, a K⁺(ATP) blocker, and indomethacin in the model of ethanol-induced gastric lesions. The hecogenin pretreatment normalized GSH levels and significantly reduced lipid peroxidation and nitrite levels in the stomach, as evaluated by the ethanol-induced gastric lesion model. The drug alone increased COX-2 expression and this effect was further enhanced in the presence of ethanol. It also decreased MPO release and significantly protected the gastric mucosa. In conclusion, we showed that hecogenin presents a significant gastroprotective effect that seems to be mediated by K⁺(ATP) channels opening and the COX-2/PG pathway. In addition, its antioxidant and anti-inflammatory properties may play a role in the gastroprotective drug effect. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/22426163/Effects_of_hecogenin_and_its_possible_mechanism_of_action_on_experimental_models_of_gastric_ulcer_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(12)00207-5 DB - PRIME DP - Unbound Medicine ER -