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Improvement of influenza vaccine strain A/Vietnam/1194/2004 (H5N1) growth with the neuraminidase packaging sequence from A/Puerto Rico/8/34.
Hum Vaccin Immunother. 2012 Feb; 8(2):252-9.HV

Abstract

H5N1 influenza candidate vaccine viruses were developed using the "6+2" approach. The hemagglutinin (HA) and neuraminidase (NA) genes were derived from the popular H5N1 virus and the remaining six internal segments were derived from the A/Puerto Rico/8/34 strain (H1N1, PR8). However, some of these candidate strains have been reported to produce relatively low yields in vaccine manufacture. In this study, we found that the NA vRNA of the A/Vietnam/1194/2004 strain (H5N1, VN1194) was poorly packaged into recombinant viruses with a backbone of PR8 genes, which resulted in the formation of defective virions that did not include the NA vRNA in the genome. Using recombinant DNA techniques, we constructed a chimeric NA gene with the coding region of VN1194 NA flanked by the packaging signal sequence of the PR8 NA gene (41 bp form the 3' end of the vRNA and 67 bp from the 5' end). The packaging of the NA vRNA was restored to normal levels in the recombinant viruses containing the chimeric NA gene. Recombinant viruses containing the chimeric NA replicated much better in chicken embryonated eggs than viruses with the wild-type NA from VN1194. These findings suggest a novel strategy to improve in ovo growth of vaccine strains and to increase the number of vaccine doses available to save people if a pandemic were to occur.

Authors+Show Affiliations

State Key Laboratory of Respiratory Diseases, Institute of Respiratory Diseases, Guangzhou College of Medicine, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22426370

Citation

Pan, Weiqi, et al. "Improvement of Influenza Vaccine Strain A/Vietnam/1194/2004 (H5N1) Growth With the Neuraminidase Packaging Sequence From A/Puerto Rico/8/34." Human Vaccines & Immunotherapeutics, vol. 8, no. 2, 2012, pp. 252-9.
Pan W, Dong Z, Meng W, et al. Improvement of influenza vaccine strain A/Vietnam/1194/2004 (H5N1) growth with the neuraminidase packaging sequence from A/Puerto Rico/8/34. Hum Vaccin Immunother. 2012;8(2):252-9.
Pan, W., Dong, Z., Meng, W., Zhang, W., Li, T., Li, C., Zhang, B., & Chen, L. (2012). Improvement of influenza vaccine strain A/Vietnam/1194/2004 (H5N1) growth with the neuraminidase packaging sequence from A/Puerto Rico/8/34. Human Vaccines & Immunotherapeutics, 8(2), 252-9. https://doi.org/10.4161/hv.18468
Pan W, et al. Improvement of Influenza Vaccine Strain A/Vietnam/1194/2004 (H5N1) Growth With the Neuraminidase Packaging Sequence From A/Puerto Rico/8/34. Hum Vaccin Immunother. 2012;8(2):252-9. PubMed PMID: 22426370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement of influenza vaccine strain A/Vietnam/1194/2004 (H5N1) growth with the neuraminidase packaging sequence from A/Puerto Rico/8/34. AU - Pan,Weiqi, AU - Dong,Zhenyuan, AU - Meng,Weixu, AU - Zhang,Wei, AU - Li,Ting, AU - Li,Chufang, AU - Zhang,Beiwu, AU - Chen,Ling, Y1 - 2012/02/01/ PY - 2012/3/20/entrez PY - 2012/3/20/pubmed PY - 2012/11/9/medline SP - 252 EP - 9 JF - Human vaccines & immunotherapeutics JO - Hum Vaccin Immunother VL - 8 IS - 2 N2 - H5N1 influenza candidate vaccine viruses were developed using the "6+2" approach. The hemagglutinin (HA) and neuraminidase (NA) genes were derived from the popular H5N1 virus and the remaining six internal segments were derived from the A/Puerto Rico/8/34 strain (H1N1, PR8). However, some of these candidate strains have been reported to produce relatively low yields in vaccine manufacture. In this study, we found that the NA vRNA of the A/Vietnam/1194/2004 strain (H5N1, VN1194) was poorly packaged into recombinant viruses with a backbone of PR8 genes, which resulted in the formation of defective virions that did not include the NA vRNA in the genome. Using recombinant DNA techniques, we constructed a chimeric NA gene with the coding region of VN1194 NA flanked by the packaging signal sequence of the PR8 NA gene (41 bp form the 3' end of the vRNA and 67 bp from the 5' end). The packaging of the NA vRNA was restored to normal levels in the recombinant viruses containing the chimeric NA gene. Recombinant viruses containing the chimeric NA replicated much better in chicken embryonated eggs than viruses with the wild-type NA from VN1194. These findings suggest a novel strategy to improve in ovo growth of vaccine strains and to increase the number of vaccine doses available to save people if a pandemic were to occur. SN - 2164-554X UR - https://www.unboundmedicine.com/medline/citation/22426370/Improvement_of_influenza_vaccine_strain_A/Vietnam/1194/2004__H5N1__growth_with_the_neuraminidase_packaging_sequence_from_A/Puerto_Rico/8/34_ L2 - https://www.tandfonline.com/doi/full/10.4161/hv.18468 DB - PRIME DP - Unbound Medicine ER -