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Presence of IgA and IgG antigliadin antibodies in healthy adults as measured by micro-ELISA. Effect of various cutoff levels on specificity and sensitivity when diagnosing coeliac disease.
Int Arch Allergy Appl Immunol 1990; 92(2):119-23IA

Abstract

In this study a micro-ELISA (ELISA = enzyme-linked immunosorbent assay) was established and used to evaluate IgA and IgG antigliadin antibodies in 1,866 healthy adults. There was a covariation between the level of IgA antigliadin antibodies and the total serum IgA concentration, probably due to an increased IgA response in some healthy subjects. We could not find any correlation between the presence of IgG and IgA antibodies in the healthy population using the 97.5th percentile as a cutoff value. The specificity of various cutoff levels was compared with the sensitivity of the test in a population of 40 patients with coeliac disease. IgA antigliadin antibodies had a high specificity (95%) at a cutoff value giving a high sensitivity (80%). This was not possible with IgG antigliadin antibodies which had a low sensitivity (40%) when the cutoff value was selected to give a high specificity. Due to the low prevalence of coeliac disease, a decrease in the specificity of the test will have a pronounced effect on the positive predictive value. The results indicate that only IgA antigliadin antibodies are useful markers when screening subjects with few typical symptoms for biopsy when diagnosing coeliac disease, whereas IgG antibodies are of low value because of their low specificity.

Authors+Show Affiliations

Clinical Immunology Laboratory, Linköping University Medical School, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2242925

Citation

Grodzinsky, E, et al. "Presence of IgA and IgG Antigliadin Antibodies in Healthy Adults as Measured By micro-ELISA. Effect of Various Cutoff Levels On Specificity and Sensitivity when Diagnosing Coeliac Disease." International Archives of Allergy and Applied Immunology, vol. 92, no. 2, 1990, pp. 119-23.
Grodzinsky E, Hed J, Liedén G, et al. Presence of IgA and IgG antigliadin antibodies in healthy adults as measured by micro-ELISA. Effect of various cutoff levels on specificity and sensitivity when diagnosing coeliac disease. Int Arch Allergy Appl Immunol. 1990;92(2):119-23.
Grodzinsky, E., Hed, J., Liedén, G., Sjögren, F., & Ström, M. (1990). Presence of IgA and IgG antigliadin antibodies in healthy adults as measured by micro-ELISA. Effect of various cutoff levels on specificity and sensitivity when diagnosing coeliac disease. International Archives of Allergy and Applied Immunology, 92(2), pp. 119-23.
Grodzinsky E, et al. Presence of IgA and IgG Antigliadin Antibodies in Healthy Adults as Measured By micro-ELISA. Effect of Various Cutoff Levels On Specificity and Sensitivity when Diagnosing Coeliac Disease. Int Arch Allergy Appl Immunol. 1990;92(2):119-23. PubMed PMID: 2242925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of IgA and IgG antigliadin antibodies in healthy adults as measured by micro-ELISA. Effect of various cutoff levels on specificity and sensitivity when diagnosing coeliac disease. AU - Grodzinsky,E, AU - Hed,J, AU - Liedén,G, AU - Sjögren,F, AU - Ström,M, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 119 EP - 23 JF - International archives of allergy and applied immunology JO - Int. Arch. Allergy Appl. Immunol. VL - 92 IS - 2 N2 - In this study a micro-ELISA (ELISA = enzyme-linked immunosorbent assay) was established and used to evaluate IgA and IgG antigliadin antibodies in 1,866 healthy adults. There was a covariation between the level of IgA antigliadin antibodies and the total serum IgA concentration, probably due to an increased IgA response in some healthy subjects. We could not find any correlation between the presence of IgG and IgA antibodies in the healthy population using the 97.5th percentile as a cutoff value. The specificity of various cutoff levels was compared with the sensitivity of the test in a population of 40 patients with coeliac disease. IgA antigliadin antibodies had a high specificity (95%) at a cutoff value giving a high sensitivity (80%). This was not possible with IgG antigliadin antibodies which had a low sensitivity (40%) when the cutoff value was selected to give a high specificity. Due to the low prevalence of coeliac disease, a decrease in the specificity of the test will have a pronounced effect on the positive predictive value. The results indicate that only IgA antigliadin antibodies are useful markers when screening subjects with few typical symptoms for biopsy when diagnosing coeliac disease, whereas IgG antibodies are of low value because of their low specificity. SN - 0020-5915 UR - https://www.unboundmedicine.com/medline/citation/2242925/Presence_of_IgA_and_IgG_antigliadin_antibodies_in_healthy_adults_as_measured_by_micro_ELISA__Effect_of_various_cutoff_levels_on_specificity_and_sensitivity_when_diagnosing_coeliac_disease_ L2 - https://medlineplus.gov/celiacdisease.html DB - PRIME DP - Unbound Medicine ER -