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Probiotics are effective for the prevention and treatment of Citrobacter rodentium-induced colitis in mice.
J Infect Dis. 2012 Jul 01; 206(1):99-109.JI

Abstract

BACKGROUND

Probiotics prevent disease induced by Citrobacter rodentium, a murine-specific enteric pathogen. Whether probiotics can be used to interrupt the infectious process following initiation of infection was determined.

METHODS

C57BL/6 adult and neonatal mice were challenged with C. rodentium, and a probiotic mixture containing Lactobacillus helveticus and Lactobacillus rhamnosus was provided 1 week before bacterial challenge, concurrently with infection, or 3 days and 6 days after infection. Mice were sacrificed 10 days after infection, and disease severity was assessed by histological analysis and in vivo intestinal permeability assay. Inflammatory pathways and the composition of the fecal microbiome were assessed in adult mice.

RESULTS

Preadministration and coadministration of probiotics ameliorated C. rodentium-induced barrier dysfunction, epithelial hyperplasia, and binding of the pathogen to host colonocytes in adults, with similar findings in neonatal mice. Upregulated tumor necrosis factor α and interferon γ transcripts were suppressed in the pretreated probiotic group, whereas interleukin 17 transcription was suppressed with probiotics given up to 3 days after infection. Probiotics promoted transcription of interleukin 10 and FOXP3, and increased follicular T-regulatory cells in pretreatment mice. C. rodentium infection resulted in an altered fecal microbiome, which was normalized with probiotic intervention.

CONCLUSIONS

This study provides evidence that probiotics can prevent illness and treat disease in an animal model of infectious colitis.

Authors+Show Affiliations

Research Institute, Hospital for Sick Children, University of Toronto, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22430833

Citation

Rodrigues, David M., et al. "Probiotics Are Effective for the Prevention and Treatment of Citrobacter Rodentium-induced Colitis in Mice." The Journal of Infectious Diseases, vol. 206, no. 1, 2012, pp. 99-109.
Rodrigues DM, Sousa AJ, Johnson-Henry KC, et al. Probiotics are effective for the prevention and treatment of Citrobacter rodentium-induced colitis in mice. J Infect Dis. 2012;206(1):99-109.
Rodrigues, D. M., Sousa, A. J., Johnson-Henry, K. C., Sherman, P. M., & Gareau, M. G. (2012). Probiotics are effective for the prevention and treatment of Citrobacter rodentium-induced colitis in mice. The Journal of Infectious Diseases, 206(1), 99-109. https://doi.org/10.1093/infdis/jis177
Rodrigues DM, et al. Probiotics Are Effective for the Prevention and Treatment of Citrobacter Rodentium-induced Colitis in Mice. J Infect Dis. 2012 Jul 1;206(1):99-109. PubMed PMID: 22430833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Probiotics are effective for the prevention and treatment of Citrobacter rodentium-induced colitis in mice. AU - Rodrigues,David M, AU - Sousa,Andrew J, AU - Johnson-Henry,Kathene C, AU - Sherman,Philip M, AU - Gareau,Mélanie G, Y1 - 2012/03/19/ PY - 2012/3/21/entrez PY - 2012/3/21/pubmed PY - 2012/10/2/medline SP - 99 EP - 109 JF - The Journal of infectious diseases JO - J Infect Dis VL - 206 IS - 1 N2 - BACKGROUND: Probiotics prevent disease induced by Citrobacter rodentium, a murine-specific enteric pathogen. Whether probiotics can be used to interrupt the infectious process following initiation of infection was determined. METHODS: C57BL/6 adult and neonatal mice were challenged with C. rodentium, and a probiotic mixture containing Lactobacillus helveticus and Lactobacillus rhamnosus was provided 1 week before bacterial challenge, concurrently with infection, or 3 days and 6 days after infection. Mice were sacrificed 10 days after infection, and disease severity was assessed by histological analysis and in vivo intestinal permeability assay. Inflammatory pathways and the composition of the fecal microbiome were assessed in adult mice. RESULTS: Preadministration and coadministration of probiotics ameliorated C. rodentium-induced barrier dysfunction, epithelial hyperplasia, and binding of the pathogen to host colonocytes in adults, with similar findings in neonatal mice. Upregulated tumor necrosis factor α and interferon γ transcripts were suppressed in the pretreated probiotic group, whereas interleukin 17 transcription was suppressed with probiotics given up to 3 days after infection. Probiotics promoted transcription of interleukin 10 and FOXP3, and increased follicular T-regulatory cells in pretreatment mice. C. rodentium infection resulted in an altered fecal microbiome, which was normalized with probiotic intervention. CONCLUSIONS: This study provides evidence that probiotics can prevent illness and treat disease in an animal model of infectious colitis. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/22430833/Probiotics_are_effective_for_the_prevention_and_treatment_of_Citrobacter_rodentium_induced_colitis_in_mice_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jis177 DB - PRIME DP - Unbound Medicine ER -