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Earthworms and humans in vitro: characterizing evolutionarily conserved stress and immune responses to silver nanoparticles.
Environ Sci Technol. 2012 Apr 03; 46(7):4166-73.ES

Abstract

Little is known about the potential threats of silver nanoparticles (AgNPs) to ecosystem health, with no detailed report existing on the stress and immune responses of soil invertebrates. Here we use earthworm primary cells, cross-referencing to human cell cultures with a particular emphasis on the conserved biological processes, and provide the first in vitro analysis of molecular and cellular toxicity mechanisms in the earthworm Eisenia fetida exposed to AgNPs (83 ± 22 nm). While we observed a clear difference in cytotoxicity of dissolved silver salt on earthworm coelomocytes and human cells (THP-1 cells, differentiated THP-1 cells and peripheral blood mononuclear cells), the coelomocytes and differentiated (macrophage-like) THP-1 cells showed a similar response to AgNPs. Intracellular accumulation of AgNPs in the coelomocytes, predominantly in a phagocytic population, was evident by several methods including transmission electron microscopy. Molecular signatures of oxidative stress and selected biomarker genes probed in a time-resolved manner suggest early regulation of oxidative stress genes and subsequent alteration of immune signaling processes following the onset of AgNP exposure in the coelomocytes and THP-1 cells. Our findings provide mechanistic clues on cellular innate immunity toward AgNPs that is likely to be evolutionarily conserved across the animal kingdom.

Authors+Show Affiliations

iNANO Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark. yuya@inano.au.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22432789

Citation

Hayashi, Yuya, et al. "Earthworms and Humans in Vitro: Characterizing Evolutionarily Conserved Stress and Immune Responses to Silver Nanoparticles." Environmental Science & Technology, vol. 46, no. 7, 2012, pp. 4166-73.
Hayashi Y, Engelmann P, Foldbjerg R, et al. Earthworms and humans in vitro: characterizing evolutionarily conserved stress and immune responses to silver nanoparticles. Environ Sci Technol. 2012;46(7):4166-73.
Hayashi, Y., Engelmann, P., Foldbjerg, R., Szabó, M., Somogyi, I., Pollák, E., Molnár, L., Autrup, H., Sutherland, D. S., Scott-Fordsmand, J., & Heckmann, L. H. (2012). Earthworms and humans in vitro: characterizing evolutionarily conserved stress and immune responses to silver nanoparticles. Environmental Science & Technology, 46(7), 4166-73. https://doi.org/10.1021/es3000905
Hayashi Y, et al. Earthworms and Humans in Vitro: Characterizing Evolutionarily Conserved Stress and Immune Responses to Silver Nanoparticles. Environ Sci Technol. 2012 Apr 3;46(7):4166-73. PubMed PMID: 22432789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Earthworms and humans in vitro: characterizing evolutionarily conserved stress and immune responses to silver nanoparticles. AU - Hayashi,Yuya, AU - Engelmann,Péter, AU - Foldbjerg,Rasmus, AU - Szabó,Mariann, AU - Somogyi,Ildikó, AU - Pollák,Edit, AU - Molnár,László, AU - Autrup,Herman, AU - Sutherland,Duncan S, AU - Scott-Fordsmand,Janeck, AU - Heckmann,Lars-Henrik, Y1 - 2012/03/20/ PY - 2012/3/22/entrez PY - 2012/3/22/pubmed PY - 2012/9/14/medline SP - 4166 EP - 73 JF - Environmental science & technology JO - Environ Sci Technol VL - 46 IS - 7 N2 - Little is known about the potential threats of silver nanoparticles (AgNPs) to ecosystem health, with no detailed report existing on the stress and immune responses of soil invertebrates. Here we use earthworm primary cells, cross-referencing to human cell cultures with a particular emphasis on the conserved biological processes, and provide the first in vitro analysis of molecular and cellular toxicity mechanisms in the earthworm Eisenia fetida exposed to AgNPs (83 ± 22 nm). While we observed a clear difference in cytotoxicity of dissolved silver salt on earthworm coelomocytes and human cells (THP-1 cells, differentiated THP-1 cells and peripheral blood mononuclear cells), the coelomocytes and differentiated (macrophage-like) THP-1 cells showed a similar response to AgNPs. Intracellular accumulation of AgNPs in the coelomocytes, predominantly in a phagocytic population, was evident by several methods including transmission electron microscopy. Molecular signatures of oxidative stress and selected biomarker genes probed in a time-resolved manner suggest early regulation of oxidative stress genes and subsequent alteration of immune signaling processes following the onset of AgNP exposure in the coelomocytes and THP-1 cells. Our findings provide mechanistic clues on cellular innate immunity toward AgNPs that is likely to be evolutionarily conserved across the animal kingdom. SN - 1520-5851 UR - https://www.unboundmedicine.com/medline/citation/22432789/Earthworms_and_humans_in_vitro:_characterizing_evolutionarily_conserved_stress_and_immune_responses_to_silver_nanoparticles_ L2 - https://doi.org/10.1021/es3000905 DB - PRIME DP - Unbound Medicine ER -