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Detection of SERS active labelled DNA based on surface affinity to silver nanoparticles.
Analyst. 2012 May 07; 137(9):2063-8.A

Abstract

Developments in specific DNA detection assays have been shown to be increasingly beneficial for molecular diagnostics and biological research. Many approaches use optical spectroscopy as an assay detection method and, owing to the sensitivity and molecular specificity offered, surface enhanced Raman scattering (SERS) spectroscopy has become a competitively exploited technique. This study utilises SERS to demonstrate differences in affinity of dye labelled DNA through differences in electrostatic interactions with silver nanoparticles. Results show clear differences in the SERS intensity obtained from single stranded DNA, double stranded DNA and a free dye label and demonstrate surface attraction is driven through electrostatic charges on the nucleotides and not the SERS dye. It has been further demonstrated that, through optimisation of experimental conditions and careful consideration of sequence composition, a DNA detection method with increased sample discrimination at lower DNA concentrations can be achieved.

Authors+Show Affiliations

Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Catherdral St., Glasgow, G1 1XL, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22434199

Citation

Harper, Mhairi M., et al. "Detection of SERS Active Labelled DNA Based On Surface Affinity to Silver Nanoparticles." The Analyst, vol. 137, no. 9, 2012, pp. 2063-8.
Harper MM, Dougan JA, Shand NC, et al. Detection of SERS active labelled DNA based on surface affinity to silver nanoparticles. Analyst. 2012;137(9):2063-8.
Harper, M. M., Dougan, J. A., Shand, N. C., Graham, D., & Faulds, K. (2012). Detection of SERS active labelled DNA based on surface affinity to silver nanoparticles. The Analyst, 137(9), 2063-8. https://doi.org/10.1039/c2an35112a
Harper MM, et al. Detection of SERS Active Labelled DNA Based On Surface Affinity to Silver Nanoparticles. Analyst. 2012 May 7;137(9):2063-8. PubMed PMID: 22434199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of SERS active labelled DNA based on surface affinity to silver nanoparticles. AU - Harper,Mhairi M, AU - Dougan,Jennifer A, AU - Shand,Neil C, AU - Graham,Duncan, AU - Faulds,Karen, Y1 - 2012/03/21/ PY - 2012/3/22/entrez PY - 2012/3/22/pubmed PY - 2012/8/9/medline SP - 2063 EP - 8 JF - The Analyst JO - Analyst VL - 137 IS - 9 N2 - Developments in specific DNA detection assays have been shown to be increasingly beneficial for molecular diagnostics and biological research. Many approaches use optical spectroscopy as an assay detection method and, owing to the sensitivity and molecular specificity offered, surface enhanced Raman scattering (SERS) spectroscopy has become a competitively exploited technique. This study utilises SERS to demonstrate differences in affinity of dye labelled DNA through differences in electrostatic interactions with silver nanoparticles. Results show clear differences in the SERS intensity obtained from single stranded DNA, double stranded DNA and a free dye label and demonstrate surface attraction is driven through electrostatic charges on the nucleotides and not the SERS dye. It has been further demonstrated that, through optimisation of experimental conditions and careful consideration of sequence composition, a DNA detection method with increased sample discrimination at lower DNA concentrations can be achieved. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22434199/Detection_of_SERS_active_labelled_DNA_based_on_surface_affinity_to_silver_nanoparticles_ L2 - https://doi.org/10.1039/c2an35112a DB - PRIME DP - Unbound Medicine ER -