Tags

Type your tag names separated by a space and hit enter

Effects of L-carnitine against oxidative stress in human hepatocytes: involvement of peroxisome proliferator-activated receptor alpha.
J Biomed Sci. 2012 Mar 21; 19:32.JB

Abstract

BACKGROUND

Excessive oxidative stress and lipid peroxidation have been demonstrated to play important roles in the production of liver damage. L-carnitine is a natural substance and acts as a carrier for fatty acids across the inner mitochondrial membrane for subsequent beta-oxidation. It is also an antioxidant that reduces metabolic stress in the cells. Recent years L-carnitine has been proposed for treatment of various kinds of disease, including liver injury. This study was conducted to evaluate the protective effect of L-carnitine against hydrogen peroxide (H2O2)-induced cytotoxicity in a normal human hepatocyte cell line, HL7702.

METHODS

We analyzed cytotoxicity using MTT assay and lactate dehydrogenase (LDH) release. Antioxidant activity and lipid peroxidation were estimated by reactive oxygen species (ROS) levels, activities and protein expressions of superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA) formation. Expressions of peroxisome proliferator-activated receptor (PPAR)-alpha and its target genes were evaluated by RT-PCR or western blotting. The role of PPAR-alpha in L-carnitine-enhanced expression of SOD and CAT was also explored. Statistical analysis was performed by a one-way analysis of variance, and its significance was assessed by Dennett's post-hoc test.

RESULTS

The results showed that L-carnitine protected HL7702 cells against cytotoxity induced by H2O2. This protection was related to the scavenging of ROS, the promotion of SOD and CAT activity and expression, and the prevention of lipid peroxidation in cultured HL7702 cells. The decreased expressions of PPAR-alpha, carnitine palmitoyl transferase 1 (CPT1) and acyl-CoA oxidase (ACOX) induced by H2O2 can be attenuated by L-carnitine. Besides, we also found that the promotion of SOD and CAT protein expression induced by L-carnitine was blocked by PPAR-alpha inhibitor MK886.

CONCLUSIONS

Taken together, our findings suggest that L-carnitine could protect HL7702 cells against oxidative stress through the antioxidative effect and the regulation of PPAR-alpha also play an important part in the protective effect.

Authors+Show Affiliations

Laboratory of Functional Physiology, Binzhou Medical University, Yantai, China. ljl813@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22435679

Citation

Li, Jin-Lian, et al. "Effects of L-carnitine Against Oxidative Stress in Human Hepatocytes: Involvement of Peroxisome Proliferator-activated Receptor Alpha." Journal of Biomedical Science, vol. 19, 2012, p. 32.
Li JL, Wang QY, Luan HY, et al. Effects of L-carnitine against oxidative stress in human hepatocytes: involvement of peroxisome proliferator-activated receptor alpha. J Biomed Sci. 2012;19:32.
Li, J. L., Wang, Q. Y., Luan, H. Y., Kang, Z. C., & Wang, C. B. (2012). Effects of L-carnitine against oxidative stress in human hepatocytes: involvement of peroxisome proliferator-activated receptor alpha. Journal of Biomedical Science, 19, 32. https://doi.org/10.1186/1423-0127-19-32
Li JL, et al. Effects of L-carnitine Against Oxidative Stress in Human Hepatocytes: Involvement of Peroxisome Proliferator-activated Receptor Alpha. J Biomed Sci. 2012 Mar 21;19:32. PubMed PMID: 22435679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of L-carnitine against oxidative stress in human hepatocytes: involvement of peroxisome proliferator-activated receptor alpha. AU - Li,Jin-Lian, AU - Wang,Qiao-Yun, AU - Luan,Hai-Yun, AU - Kang,Ze-Chun, AU - Wang,Chun-Bo, Y1 - 2012/03/21/ PY - 2012/01/03/received PY - 2012/03/21/accepted PY - 2012/3/23/entrez PY - 2012/3/23/pubmed PY - 2012/9/25/medline SP - 32 EP - 32 JF - Journal of biomedical science JO - J Biomed Sci VL - 19 N2 - BACKGROUND: Excessive oxidative stress and lipid peroxidation have been demonstrated to play important roles in the production of liver damage. L-carnitine is a natural substance and acts as a carrier for fatty acids across the inner mitochondrial membrane for subsequent beta-oxidation. It is also an antioxidant that reduces metabolic stress in the cells. Recent years L-carnitine has been proposed for treatment of various kinds of disease, including liver injury. This study was conducted to evaluate the protective effect of L-carnitine against hydrogen peroxide (H2O2)-induced cytotoxicity in a normal human hepatocyte cell line, HL7702. METHODS: We analyzed cytotoxicity using MTT assay and lactate dehydrogenase (LDH) release. Antioxidant activity and lipid peroxidation were estimated by reactive oxygen species (ROS) levels, activities and protein expressions of superoxide dismutase (SOD) and catalase (CAT), and malondialdehyde (MDA) formation. Expressions of peroxisome proliferator-activated receptor (PPAR)-alpha and its target genes were evaluated by RT-PCR or western blotting. The role of PPAR-alpha in L-carnitine-enhanced expression of SOD and CAT was also explored. Statistical analysis was performed by a one-way analysis of variance, and its significance was assessed by Dennett's post-hoc test. RESULTS: The results showed that L-carnitine protected HL7702 cells against cytotoxity induced by H2O2. This protection was related to the scavenging of ROS, the promotion of SOD and CAT activity and expression, and the prevention of lipid peroxidation in cultured HL7702 cells. The decreased expressions of PPAR-alpha, carnitine palmitoyl transferase 1 (CPT1) and acyl-CoA oxidase (ACOX) induced by H2O2 can be attenuated by L-carnitine. Besides, we also found that the promotion of SOD and CAT protein expression induced by L-carnitine was blocked by PPAR-alpha inhibitor MK886. CONCLUSIONS: Taken together, our findings suggest that L-carnitine could protect HL7702 cells against oxidative stress through the antioxidative effect and the regulation of PPAR-alpha also play an important part in the protective effect. SN - 1423-0127 UR - https://www.unboundmedicine.com/medline/citation/22435679/Effects_of_L_carnitine_against_oxidative_stress_in_human_hepatocytes:_involvement_of_peroxisome_proliferator_activated_receptor_alpha_ L2 - https://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-19-32 DB - PRIME DP - Unbound Medicine ER -