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Influence of the DNA structure on the free radical induction due to proflavine and light treatment.
Radiat Environ Biophys. 1979 Apr 30; 16(2):125-34.RE

Abstract

Induction of peroxide free radicals (detected by Electron Paramagnetic Resonance at 77 K) due to the photodynamic activity of proflavine was measured on bacteriophage phi X174 DNA either single-stranded (ss) as isolated from the virion, or double-stranded supercoiled (RFI) as isolated from the infected bacteria. Comparison was made with calf thymus DNA photosensitization. In order to use equivalent DNA-proflavine complexes, binding of the dye to the three DNA's was first determined under those conditions of high ionic strength favourable to the photodynamic reaction. Free radical induction was maximal for definite amounts of bound proflavine (which varied depending upon the DNA substrate) and at an ionic strength value of 0.5. The level of the maximal reaction increased in the following order: from phi Xss DNA to calf thymus DNA and finally to phi XRFI DNA. The conformation of the proflavine-DNA complex was thus a determinant for the efficiency of the photodynamic process. The ionic strength effect could not be explained by the evolution of the proflavine triplet state in irradiated proflavine-calf thymus DNA complexes.

Authors

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Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

224407

Citation

Piette, J, et al. "Influence of the DNA Structure On the Free Radical Induction Due to Proflavine and Light Treatment." Radiation and Environmental Biophysics, vol. 16, no. 2, 1979, pp. 125-34.
Piette J, Calberg-Bacq CM, Van de Vorst A. Influence of the DNA structure on the free radical induction due to proflavine and light treatment. Radiat Environ Biophys. 1979;16(2):125-34.
Piette, J., Calberg-Bacq, C. M., & Van de Vorst, A. (1979). Influence of the DNA structure on the free radical induction due to proflavine and light treatment. Radiation and Environmental Biophysics, 16(2), 125-34.
Piette J, Calberg-Bacq CM, Van de Vorst A. Influence of the DNA Structure On the Free Radical Induction Due to Proflavine and Light Treatment. Radiat Environ Biophys. 1979 Apr 30;16(2):125-34. PubMed PMID: 224407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of the DNA structure on the free radical induction due to proflavine and light treatment. AU - Piette,J, AU - Calberg-Bacq,C M, AU - Van de Vorst,A, PY - 1979/4/30/pubmed PY - 1979/4/30/medline PY - 1979/4/30/entrez SP - 125 EP - 34 JF - Radiation and environmental biophysics JO - Radiat Environ Biophys VL - 16 IS - 2 N2 - Induction of peroxide free radicals (detected by Electron Paramagnetic Resonance at 77 K) due to the photodynamic activity of proflavine was measured on bacteriophage phi X174 DNA either single-stranded (ss) as isolated from the virion, or double-stranded supercoiled (RFI) as isolated from the infected bacteria. Comparison was made with calf thymus DNA photosensitization. In order to use equivalent DNA-proflavine complexes, binding of the dye to the three DNA's was first determined under those conditions of high ionic strength favourable to the photodynamic reaction. Free radical induction was maximal for definite amounts of bound proflavine (which varied depending upon the DNA substrate) and at an ionic strength value of 0.5. The level of the maximal reaction increased in the following order: from phi Xss DNA to calf thymus DNA and finally to phi XRFI DNA. The conformation of the proflavine-DNA complex was thus a determinant for the efficiency of the photodynamic process. The ionic strength effect could not be explained by the evolution of the proflavine triplet state in irradiated proflavine-calf thymus DNA complexes. SN - 0301-634X UR - https://www.unboundmedicine.com/medline/citation/224407/Influence_of_the_DNA_structure_on_the_free_radical_induction_due_to_proflavine_and_light_treatment_ DB - PRIME DP - Unbound Medicine ER -