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Development of a fully automated on-line solid phase extraction and high-performance liquid chromatography with diode array detection method for the pharmacokinetic evaluation of bavachinin: a study on absolute bioavailability and dose proportionality.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 15; 893-894:21-8.JC

Abstract

A fully automated on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) with diode array detection (DAD) method was developed for determination of bavachinin in mouse plasma. Analytical process was performed on two reversed-phase columns (SPE cartridge and analytical column) connected via a Valco 6-port switching valve. Plasma samples (10 μL) were injected directly onto a C18 SPE cartridge (MF Ph-1 C18, 10 mm × 4 mm, 5 μm) and the biological matrix was washed out for 2 min with the loading solvent (5 mM NaH(2)PO(4) buffer, pH 3.5) at a flow rate of 1 mL/min. By rotation of the switching valve, bavachinin was eluted from the SPE cartridge in the back-flush mode and transferred to the analytical column (Venusil MP C18, 4.6 mm × 150 mm, 5 μm) by the chromatographic mobile phase consisted of acetonitrile-5mM NaH(2)PO(4) buffer 65/35 (v/v, pH 3.5) at a flow rate of 1 mL/min. The complete cycle of the on-line SPE purification and chromatographic separation of the analyte was 13 min with UV detection performed at 236 nm. Calibration curve with good linearity (r=0.9997) was obtained in the range of 20-4000 ng/mL in mouse plasma. The intra-day and inter-day precisions (RSD) of bavachinin were in the range of 0.20-2.32% and the accuracies were between 98.47% and 102.95%. The lower limit of quantification (LLOQ) of the assay was 20 ng/mL. In conclusion, the established automated on-line SPE-HPLC-DAD method demonstrated good performance in terms of linearity, specificity, detection and quantification limits, precision and accuracy, and was successfully utilized to quantify bavachinin in mouse plasma to support the pharmacokinetic (PK) studies. The PK properties of bavachinin were characterized as rapid oral absorption, high clearance, and poor absolute bioavailability.

Authors+Show Affiliations

College of Pharmacy & State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22444438

Citation

Liu, Lei, et al. "Development of a Fully Automated On-line Solid Phase Extraction and High-performance Liquid Chromatography With Diode Array Detection Method for the Pharmacokinetic Evaluation of Bavachinin: a Study On Absolute Bioavailability and Dose Proportionality." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 893-894, 2012, pp. 21-8.
Liu L, Liu KN, Wen YB, et al. Development of a fully automated on-line solid phase extraction and high-performance liquid chromatography with diode array detection method for the pharmacokinetic evaluation of bavachinin: a study on absolute bioavailability and dose proportionality. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;893-894:21-8.
Liu, L., Liu, K. N., Wen, Y. B., Zhang, H. W., Lu, Y. X., & Yin, Z. (2012). Development of a fully automated on-line solid phase extraction and high-performance liquid chromatography with diode array detection method for the pharmacokinetic evaluation of bavachinin: a study on absolute bioavailability and dose proportionality. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 893-894, 21-8. https://doi.org/10.1016/j.jchromb.2012.02.020
Liu L, et al. Development of a Fully Automated On-line Solid Phase Extraction and High-performance Liquid Chromatography With Diode Array Detection Method for the Pharmacokinetic Evaluation of Bavachinin: a Study On Absolute Bioavailability and Dose Proportionality. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 15;893-894:21-8. PubMed PMID: 22444438.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a fully automated on-line solid phase extraction and high-performance liquid chromatography with diode array detection method for the pharmacokinetic evaluation of bavachinin: a study on absolute bioavailability and dose proportionality. AU - Liu,Lei, AU - Liu,Kang-Ning, AU - Wen,Ya-Bin, AU - Zhang,Han-Wen, AU - Lu,Ya-Xin, AU - Yin,Zheng, Y1 - 2012/02/21/ PY - 2011/10/14/received PY - 2012/02/12/revised PY - 2012/02/13/accepted PY - 2012/3/27/entrez PY - 2012/3/27/pubmed PY - 2012/7/27/medline SP - 21 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 893-894 N2 - A fully automated on-line solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) with diode array detection (DAD) method was developed for determination of bavachinin in mouse plasma. Analytical process was performed on two reversed-phase columns (SPE cartridge and analytical column) connected via a Valco 6-port switching valve. Plasma samples (10 μL) were injected directly onto a C18 SPE cartridge (MF Ph-1 C18, 10 mm × 4 mm, 5 μm) and the biological matrix was washed out for 2 min with the loading solvent (5 mM NaH(2)PO(4) buffer, pH 3.5) at a flow rate of 1 mL/min. By rotation of the switching valve, bavachinin was eluted from the SPE cartridge in the back-flush mode and transferred to the analytical column (Venusil MP C18, 4.6 mm × 150 mm, 5 μm) by the chromatographic mobile phase consisted of acetonitrile-5mM NaH(2)PO(4) buffer 65/35 (v/v, pH 3.5) at a flow rate of 1 mL/min. The complete cycle of the on-line SPE purification and chromatographic separation of the analyte was 13 min with UV detection performed at 236 nm. Calibration curve with good linearity (r=0.9997) was obtained in the range of 20-4000 ng/mL in mouse plasma. The intra-day and inter-day precisions (RSD) of bavachinin were in the range of 0.20-2.32% and the accuracies were between 98.47% and 102.95%. The lower limit of quantification (LLOQ) of the assay was 20 ng/mL. In conclusion, the established automated on-line SPE-HPLC-DAD method demonstrated good performance in terms of linearity, specificity, detection and quantification limits, precision and accuracy, and was successfully utilized to quantify bavachinin in mouse plasma to support the pharmacokinetic (PK) studies. The PK properties of bavachinin were characterized as rapid oral absorption, high clearance, and poor absolute bioavailability. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/22444438/Development_of_a_fully_automated_on_line_solid_phase_extraction_and_high_performance_liquid_chromatography_with_diode_array_detection_method_for_the_pharmacokinetic_evaluation_of_bavachinin:_a_study_on_absolute_bioavailability_and_dose_proportionality_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(12)00108-0 DB - PRIME DP - Unbound Medicine ER -