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Clenbuterol - regional food contamination a possible source for inadvertent doping in sports.
Drug Test Anal. 2012 Jun; 4(6):534-8.DT

Abstract

The misuse of the sympathomimetic and anabolic agent clenbuterol has been frequently reported in professional sport and in the livestock industry. In 2010, a team of athletes returned from competition in China and regular doping control samples were taken within the next two days. All urine samples contained low amounts (pg/ml) of clenbuterol, drawing the attention to a well-known problem: the possibility of an unintended clenbuterol intake with food. A warning that Chinese meat is possibly contaminated with prohibited substances according to international anti-doping regulations was also given by Chinese officials just before the Bejing Olympic Games in 2008. To investigate if clenbuterol can be found in human urine, a study was initiated comprising 28 volunteers collecting urine samples after their return from China. For the quantification of clenbuterol at a low pg/ml level, a very sensitive and specific isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed using liquid/liquid re-extraction for clean-up with a limit of detection and quantification of 1 and 3 pg/ml, respectively. The method was validated demonstrating good precision (intra-day: 2.9-5.5 %; inter-day: 5.1-8.8%), accuracy (89.5-102.5%) and mean recovery (81.4%). Clenbuterol was detectable in 22 (79%) of the analyzed samples, indicating a general food contamination problem despite an official clenbuterol prohibition in China for livestock.

Authors+Show Affiliations

Institute of Biochemistry and Center of Preventive Doping Research, German Sport University Cologne, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

22447758

Citation

Guddat, S, et al. "Clenbuterol - Regional Food Contamination a Possible Source for Inadvertent Doping in Sports." Drug Testing and Analysis, vol. 4, no. 6, 2012, pp. 534-8.
Guddat S, Fuβhöller G, Geyer H, et al. Clenbuterol - regional food contamination a possible source for inadvertent doping in sports. Drug Test Anal. 2012;4(6):534-8.
Guddat, S., Fuβhöller, G., Geyer, H., Thomas, A., Braun, H., Haenelt, N., Schwenke, A., Klose, C., Thevis, M., & Schänzer, W. (2012). Clenbuterol - regional food contamination a possible source for inadvertent doping in sports. Drug Testing and Analysis, 4(6), 534-8. https://doi.org/10.1002/dta.1330
Guddat S, et al. Clenbuterol - Regional Food Contamination a Possible Source for Inadvertent Doping in Sports. Drug Test Anal. 2012;4(6):534-8. PubMed PMID: 22447758.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clenbuterol - regional food contamination a possible source for inadvertent doping in sports. AU - Guddat,S, AU - Fuβhöller,G, AU - Geyer,H, AU - Thomas,A, AU - Braun,H, AU - Haenelt,N, AU - Schwenke,A, AU - Klose,C, AU - Thevis,M, AU - Schänzer,W, Y1 - 2012/03/22/ PY - 2011/12/12/received PY - 2012/01/17/accepted PY - 2012/3/27/entrez PY - 2012/3/27/pubmed PY - 2012/11/6/medline SP - 534 EP - 8 JF - Drug testing and analysis JO - Drug Test Anal VL - 4 IS - 6 N2 - The misuse of the sympathomimetic and anabolic agent clenbuterol has been frequently reported in professional sport and in the livestock industry. In 2010, a team of athletes returned from competition in China and regular doping control samples were taken within the next two days. All urine samples contained low amounts (pg/ml) of clenbuterol, drawing the attention to a well-known problem: the possibility of an unintended clenbuterol intake with food. A warning that Chinese meat is possibly contaminated with prohibited substances according to international anti-doping regulations was also given by Chinese officials just before the Bejing Olympic Games in 2008. To investigate if clenbuterol can be found in human urine, a study was initiated comprising 28 volunteers collecting urine samples after their return from China. For the quantification of clenbuterol at a low pg/ml level, a very sensitive and specific isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed using liquid/liquid re-extraction for clean-up with a limit of detection and quantification of 1 and 3 pg/ml, respectively. The method was validated demonstrating good precision (intra-day: 2.9-5.5 %; inter-day: 5.1-8.8%), accuracy (89.5-102.5%) and mean recovery (81.4%). Clenbuterol was detectable in 22 (79%) of the analyzed samples, indicating a general food contamination problem despite an official clenbuterol prohibition in China for livestock. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/22447758/Clenbuterol___regional_food_contamination_a_possible_source_for_inadvertent_doping_in_sports_ L2 - https://doi.org/10.1002/dta.1330 DB - PRIME DP - Unbound Medicine ER -