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Analysis of drugs of forensic interest with capillary zone electrophoresis/time-of-flight mass spectrometry based on the use of non-volatile buffers.
Electrophoresis. 2012 Feb; 33(4):599-606.E

Abstract

The present work is aimed at investigating the influence of the background electrolyte composition and concentration on the separation efficiency and resolution and mass spectrometric detection of illicit drugs in a capillary zone electrophoresis-electrospray ionization-time of flight mass spectrometry (CZE-ESI-TOF MS) system. The effect of phosphate, borate and Tris buffers on the separation and mass spectrometry response of a mixture of 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, methadone, cocaine, morphine, codeine and 6-monoacetylmorphine was studied, in comparison with a reference ammonium formate separation buffer. Inorganic non-volatile borate and Tris buffers proved hardly suitable for capillary electrophoresis-mass spectrometry (CE-MS) analysis, but quite unexpectedly ammonium phosphate buffers showed good separation and ionization performances for all the analytes tested. Applications of this method to real samples of hair from drug addicts are also provided.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Gottardo R
Department of Public Health and Community Medicine, Unit of Forensic Medicine, University of Verona, Verona, Italy.
Mikšík I
No affiliation info available
Aturki Z
No affiliation info available
Sorio D
No affiliation info available
Seri C
No affiliation info available
Fanali S
No affiliation info available
Tagliaro F
No affiliation info available

MeSH

BuffersElectrophoresis, CapillaryForensic ToxicologyHairHumansIllicit DrugsMass SpectrometryPhosphates

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22451052

Citation

Gottardo, Rossella, et al. "Analysis of Drugs of Forensic Interest With Capillary Zone Electrophoresis/time-of-flight Mass Spectrometry Based On the Use of Non-volatile Buffers." Electrophoresis, vol. 33, no. 4, 2012, pp. 599-606.
Gottardo R, Mikšík I, Aturki Z, et al. Analysis of drugs of forensic interest with capillary zone electrophoresis/time-of-flight mass spectrometry based on the use of non-volatile buffers. Electrophoresis. 2012;33(4):599-606.
Gottardo, R., Mikšík, I., Aturki, Z., Sorio, D., Seri, C., Fanali, S., & Tagliaro, F. (2012). Analysis of drugs of forensic interest with capillary zone electrophoresis/time-of-flight mass spectrometry based on the use of non-volatile buffers. Electrophoresis, 33(4), 599-606. https://doi.org/10.1002/elps.201100383
Gottardo R, et al. Analysis of Drugs of Forensic Interest With Capillary Zone Electrophoresis/time-of-flight Mass Spectrometry Based On the Use of Non-volatile Buffers. Electrophoresis. 2012;33(4):599-606. PubMed PMID: 22451052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of drugs of forensic interest with capillary zone electrophoresis/time-of-flight mass spectrometry based on the use of non-volatile buffers. AU - Gottardo,Rossella, AU - Mikšík,Ivan, AU - Aturki,Zeineb, AU - Sorio,Daniela, AU - Seri,Catia, AU - Fanali,Salvatore, AU - Tagliaro,Franco, PY - 2012/3/28/entrez PY - 2012/3/28/pubmed PY - 2012/6/20/medline SP - 599 EP - 606 JF - Electrophoresis JO - Electrophoresis VL - 33 IS - 4 N2 - The present work is aimed at investigating the influence of the background electrolyte composition and concentration on the separation efficiency and resolution and mass spectrometric detection of illicit drugs in a capillary zone electrophoresis-electrospray ionization-time of flight mass spectrometry (CZE-ESI-TOF MS) system. The effect of phosphate, borate and Tris buffers on the separation and mass spectrometry response of a mixture of 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, methadone, cocaine, morphine, codeine and 6-monoacetylmorphine was studied, in comparison with a reference ammonium formate separation buffer. Inorganic non-volatile borate and Tris buffers proved hardly suitable for capillary electrophoresis-mass spectrometry (CE-MS) analysis, but quite unexpectedly ammonium phosphate buffers showed good separation and ionization performances for all the analytes tested. Applications of this method to real samples of hair from drug addicts are also provided. SN - 1522-2683 UR - https://www.unboundmedicine.com/medline/citation/22451052/Analysis_of_drugs_of_forensic_interest_with_capillary_zone_electrophoresis/time_of_flight_mass_spectrometry_based_on_the_use_of_non_volatile_buffers_ L2 - https://doi.org/10.1002/elps.201100383 DB - PRIME DP - Unbound Medicine ER -