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Antioxidant/antibacterial activities of a topical phytopharmaceutical formulation containing a standardized extract of Baccharis incarum, an extremophile plant species from Argentine Puna.
Phytother Res. 2012 Nov; 26(11):1759-67.PR

Abstract

The antimicrobial and antioxidant activities of standardized extracts of Baccharis incarum in 60° and 80° ethanol and of a phytopharmaceutical formulation obtained from them were measured. Baccharis tinctures showed antimicrobial activity against clinically isolated antibiotic resistant Staphylococcus aureus and Enterococcus faecalis, with MIC values of 40-80 µg GAE/mL. Both tinctures exhibited ABTS(●+) scavenging activity with SC(50) values between 1.6 and 4.0 µg GAE/mL. The tinctures were not genotoxic in the Salmonella assay. For this reason, the tincture in 60° ethanol was incorporated into a topical pharmaceutical formulation (Hydrogel/ Carbopol® 934). The phytopharmaceutical formulation also showed antibacterial and antioxidant activities in the in vitro assays. The hydrogel showed microbiological, chemical, physical and functional stability during storage at room temperature. Studies that measure drug release as a determination of bioavailability were also carried out using the Franz diffusion cell (FC). The results demonstrated the release of two bioactive compounds (chlorogenic acid and 4',5-dihydroxy-3',3,6,7,8-pentamethoxyflavone) from the phytotherapic preparation in HPLC studies of FC receptor solution. In consequence, the phytopreparation applied topically could be used to treat skin and soft tissue infection produced by methicillin-resistant Staphylococcus aureus (MRSA) or Enterococcus faecalis and opens new opportunities for the use of active natural ingredients in the cosmeceutical field as antiacne and antioxidant.

Authors+Show Affiliations

INQUINOA (CONICET), San Miguel de Tucumán, Tucumán, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22451265

Citation

Nuño, Gabriela, et al. "Antioxidant/antibacterial Activities of a Topical Phytopharmaceutical Formulation Containing a Standardized Extract of Baccharis Incarum, an Extremophile Plant Species From Argentine Puna." Phytotherapy Research : PTR, vol. 26, no. 11, 2012, pp. 1759-67.
Nuño G, Zampini IC, Ordoñez RM, et al. Antioxidant/antibacterial activities of a topical phytopharmaceutical formulation containing a standardized extract of Baccharis incarum, an extremophile plant species from Argentine Puna. Phytother Res. 2012;26(11):1759-67.
Nuño, G., Zampini, I. C., Ordoñez, R. M., Alberto, M. R., Arias, M. E., & Isla, M. I. (2012). Antioxidant/antibacterial activities of a topical phytopharmaceutical formulation containing a standardized extract of Baccharis incarum, an extremophile plant species from Argentine Puna. Phytotherapy Research : PTR, 26(11), 1759-67. https://doi.org/10.1002/ptr.4633
Nuño G, et al. Antioxidant/antibacterial Activities of a Topical Phytopharmaceutical Formulation Containing a Standardized Extract of Baccharis Incarum, an Extremophile Plant Species From Argentine Puna. Phytother Res. 2012;26(11):1759-67. PubMed PMID: 22451265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant/antibacterial activities of a topical phytopharmaceutical formulation containing a standardized extract of Baccharis incarum, an extremophile plant species from Argentine Puna. AU - Nuño,Gabriela, AU - Zampini,Iris C, AU - Ordoñez,Roxana M, AU - Alberto,María R, AU - Arias,Myriam E, AU - Isla,María I, Y1 - 2012/03/26/ PY - 2011/11/14/received PY - 2012/01/26/accepted PY - 2012/3/28/entrez PY - 2012/3/28/pubmed PY - 2013/1/17/medline SP - 1759 EP - 67 JF - Phytotherapy research : PTR JO - Phytother Res VL - 26 IS - 11 N2 - The antimicrobial and antioxidant activities of standardized extracts of Baccharis incarum in 60° and 80° ethanol and of a phytopharmaceutical formulation obtained from them were measured. Baccharis tinctures showed antimicrobial activity against clinically isolated antibiotic resistant Staphylococcus aureus and Enterococcus faecalis, with MIC values of 40-80 µg GAE/mL. Both tinctures exhibited ABTS(●+) scavenging activity with SC(50) values between 1.6 and 4.0 µg GAE/mL. The tinctures were not genotoxic in the Salmonella assay. For this reason, the tincture in 60° ethanol was incorporated into a topical pharmaceutical formulation (Hydrogel/ Carbopol® 934). The phytopharmaceutical formulation also showed antibacterial and antioxidant activities in the in vitro assays. The hydrogel showed microbiological, chemical, physical and functional stability during storage at room temperature. Studies that measure drug release as a determination of bioavailability were also carried out using the Franz diffusion cell (FC). The results demonstrated the release of two bioactive compounds (chlorogenic acid and 4',5-dihydroxy-3',3,6,7,8-pentamethoxyflavone) from the phytotherapic preparation in HPLC studies of FC receptor solution. In consequence, the phytopreparation applied topically could be used to treat skin and soft tissue infection produced by methicillin-resistant Staphylococcus aureus (MRSA) or Enterococcus faecalis and opens new opportunities for the use of active natural ingredients in the cosmeceutical field as antiacne and antioxidant. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/22451265/Antioxidant/antibacterial_activities_of_a_topical_phytopharmaceutical_formulation_containing_a_standardized_extract_of_Baccharis_incarum_an_extremophile_plant_species_from_Argentine_Puna_ L2 - https://doi.org/10.1002/ptr.4633 DB - PRIME DP - Unbound Medicine ER -