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The effects of peptide and lipid endocannabinoids on arthritic pain at the spinal level.
Anesth Analg. 2012 Jun; 114(6):1346-52.A&A

Abstract

BACKGROUND

Hemopressin, a nonapeptide (PVNFKFLSH: HP) derived from the α chain of hemoglobin was shown to interact specifically with brain cannabinoid CB(1) receptors. Therefore, it seems to be the only peptide structure with cannabinoid activities. Our goal in this study was to further characterize this peptide and to clarify the antinociceptive potency of the polyunsaturated fatty acid derivates, 2-arachidonoyl-glycerol (2-AG) and anandamide, by investigating their effects on mechanical allodynia at the spinal level.

METHODS

HP was prepared on solid phase by in situ neutralization. After chronic intrathecal catheterization, mechanical hypersensitivity was produced in male Wistar rats by injection of carrageenan (300 μg/30 μL) into the tibiotarsal joint of one of the hind legs. Three hours after carrageenan administration, the ligands were administered intrathecally. The mechanical threshold was assessed using a dynamic aesthesiometer.

RESULTS

2-AG (1-200 μg) and anandamide (10-200 μg) decreased carrageenan-induced mechanical allodynia in a dose-dependent manner, whereas HP had no antinociceptive effect in a wide dose range (0.3-30 μg). The effect of 2-AG was prevented by the CB(1) receptor antagonist AM 251, but not by the CB(2) antagonist SSR144528-2. HP (3 and 30 μg) also inhibited the effect of 2-AG. None of the ligands influenced the degree of edema.

CONCLUSIONS

HP posttreatment had no effect on mechanical allodynia, whereas spinally injected 2-AG and anandamide were potent drugs.

Authors+Show Affiliations

Department of Physiology, Faculty of Medicine, University of Szeged, Szeged, Hungary.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22451592

Citation

Petrovszki, Zita, et al. "The Effects of Peptide and Lipid Endocannabinoids On Arthritic Pain at the Spinal Level." Anesthesia and Analgesia, vol. 114, no. 6, 2012, pp. 1346-52.
Petrovszki Z, Kovacs G, Tömböly C, et al. The effects of peptide and lipid endocannabinoids on arthritic pain at the spinal level. Anesth Analg. 2012;114(6):1346-52.
Petrovszki, Z., Kovacs, G., Tömböly, C., Benedek, G., & Horvath, G. (2012). The effects of peptide and lipid endocannabinoids on arthritic pain at the spinal level. Anesthesia and Analgesia, 114(6), 1346-52. https://doi.org/10.1213/ANE.0b013e31824c4eeb
Petrovszki Z, et al. The Effects of Peptide and Lipid Endocannabinoids On Arthritic Pain at the Spinal Level. Anesth Analg. 2012;114(6):1346-52. PubMed PMID: 22451592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of peptide and lipid endocannabinoids on arthritic pain at the spinal level. AU - Petrovszki,Zita, AU - Kovacs,Gyula, AU - Tömböly,Csaba, AU - Benedek,György, AU - Horvath,Gyongyi, Y1 - 2012/03/26/ PY - 2012/3/28/entrez PY - 2012/3/28/pubmed PY - 2012/7/20/medline SP - 1346 EP - 52 JF - Anesthesia and analgesia JO - Anesth Analg VL - 114 IS - 6 N2 - BACKGROUND: Hemopressin, a nonapeptide (PVNFKFLSH: HP) derived from the α chain of hemoglobin was shown to interact specifically with brain cannabinoid CB(1) receptors. Therefore, it seems to be the only peptide structure with cannabinoid activities. Our goal in this study was to further characterize this peptide and to clarify the antinociceptive potency of the polyunsaturated fatty acid derivates, 2-arachidonoyl-glycerol (2-AG) and anandamide, by investigating their effects on mechanical allodynia at the spinal level. METHODS: HP was prepared on solid phase by in situ neutralization. After chronic intrathecal catheterization, mechanical hypersensitivity was produced in male Wistar rats by injection of carrageenan (300 μg/30 μL) into the tibiotarsal joint of one of the hind legs. Three hours after carrageenan administration, the ligands were administered intrathecally. The mechanical threshold was assessed using a dynamic aesthesiometer. RESULTS: 2-AG (1-200 μg) and anandamide (10-200 μg) decreased carrageenan-induced mechanical allodynia in a dose-dependent manner, whereas HP had no antinociceptive effect in a wide dose range (0.3-30 μg). The effect of 2-AG was prevented by the CB(1) receptor antagonist AM 251, but not by the CB(2) antagonist SSR144528-2. HP (3 and 30 μg) also inhibited the effect of 2-AG. None of the ligands influenced the degree of edema. CONCLUSIONS: HP posttreatment had no effect on mechanical allodynia, whereas spinally injected 2-AG and anandamide were potent drugs. SN - 1526-7598 UR - https://www.unboundmedicine.com/medline/citation/22451592/The_effects_of_peptide_and_lipid_endocannabinoids_on_arthritic_pain_at_the_spinal_level_ L2 - https://doi.org/10.1213/ANE.0b013e31824c4eeb DB - PRIME DP - Unbound Medicine ER -