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CHD3 protein recognizes and regulates methylated histone H3 lysines 4 and 27 over a subset of targets in the rice genome.
Proc Natl Acad Sci U S A. 2012 Apr 10; 109(15):5773-8.PN

Abstract

Histone lysine methylation is an important component of the epigenetic system demarcating transcriptionally active and inactive chromatin domains. It is of primary importance in understanding how different histone lysine methylation marks and a specific combination of them are read and interpreted by chromatin proteins to regulate gene expression. In this paper, we report that the rice CHD3 protein CHR729 that was required for many aspects of plant development can interact with dimethylated histone H3 lysine 4 (H3K4me2, a mark associated with moderately expressed or repressed genes) and with trimethylated histone H3 lysine 27 (H3K27me3, a mark associated with repressed genes), respectively, through the chromodomains and the plant homeodomain (PHD) finger of the protein. A mutation or down-regulation of the gene provoked a decrease of H3K27me3 and H3K4me3 (a mark associated with active genes). Genome-wide analysis revealed that H3K27me3 and H3K4me3, respectively, were lost from about 56 and 23% of marked loci, which correspond mostly to under-expressed or repressed genes. In the mutant, a higher-than-expected proportion of down-regulated genes lost H3K4me3, among which many encode DNA-binding transcription factors. These results suggest that the rice CHD3 protein is a bifunctional chromatin regulator able to recognize and modulate H3K4 and H3K27 methylation over repressed or tissue-specific genes, which may be associated with regulation of a gene transcription program of plant development.

Authors+Show Affiliations

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22451926

Citation

Hu, Yongfeng, et al. "CHD3 Protein Recognizes and Regulates Methylated Histone H3 Lysines 4 and 27 Over a Subset of Targets in the Rice Genome." Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 15, 2012, pp. 5773-8.
Hu Y, Liu D, Zhong X, et al. CHD3 protein recognizes and regulates methylated histone H3 lysines 4 and 27 over a subset of targets in the rice genome. Proc Natl Acad Sci USA. 2012;109(15):5773-8.
Hu, Y., Liu, D., Zhong, X., Zhang, C., Zhang, Q., & Zhou, D. X. (2012). CHD3 protein recognizes and regulates methylated histone H3 lysines 4 and 27 over a subset of targets in the rice genome. Proceedings of the National Academy of Sciences of the United States of America, 109(15), 5773-8. https://doi.org/10.1073/pnas.1203148109
Hu Y, et al. CHD3 Protein Recognizes and Regulates Methylated Histone H3 Lysines 4 and 27 Over a Subset of Targets in the Rice Genome. Proc Natl Acad Sci USA. 2012 Apr 10;109(15):5773-8. PubMed PMID: 22451926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CHD3 protein recognizes and regulates methylated histone H3 lysines 4 and 27 over a subset of targets in the rice genome. AU - Hu,Yongfeng, AU - Liu,Dengnian, AU - Zhong,Xiaocao, AU - Zhang,Chengjun, AU - Zhang,Qifa, AU - Zhou,Dao-Xiu, Y1 - 2012/03/26/ PY - 2012/3/28/entrez PY - 2012/3/28/pubmed PY - 2012/6/5/medline SP - 5773 EP - 8 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 109 IS - 15 N2 - Histone lysine methylation is an important component of the epigenetic system demarcating transcriptionally active and inactive chromatin domains. It is of primary importance in understanding how different histone lysine methylation marks and a specific combination of them are read and interpreted by chromatin proteins to regulate gene expression. In this paper, we report that the rice CHD3 protein CHR729 that was required for many aspects of plant development can interact with dimethylated histone H3 lysine 4 (H3K4me2, a mark associated with moderately expressed or repressed genes) and with trimethylated histone H3 lysine 27 (H3K27me3, a mark associated with repressed genes), respectively, through the chromodomains and the plant homeodomain (PHD) finger of the protein. A mutation or down-regulation of the gene provoked a decrease of H3K27me3 and H3K4me3 (a mark associated with active genes). Genome-wide analysis revealed that H3K27me3 and H3K4me3, respectively, were lost from about 56 and 23% of marked loci, which correspond mostly to under-expressed or repressed genes. In the mutant, a higher-than-expected proportion of down-regulated genes lost H3K4me3, among which many encode DNA-binding transcription factors. These results suggest that the rice CHD3 protein is a bifunctional chromatin regulator able to recognize and modulate H3K4 and H3K27 methylation over repressed or tissue-specific genes, which may be associated with regulation of a gene transcription program of plant development. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/22451926/CHD3_protein_recognizes_and_regulates_methylated_histone_H3_lysines_4_and_27_over_a_subset_of_targets_in_the_rice_genome_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=22451926 DB - PRIME DP - Unbound Medicine ER -